Mice that are homozygous for this targeted mutation of Mtnr1b, melatonin receptor 1B, exhibit deficits in learning and/or memory. This mutant mouse strain may be useful in studies of circadian rhythm, learning and behavior.
David R. Weaver, Univ of Massachusetts Medical School
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical abnormalities. Although circadian rhythm is normal, mice homozygous for the targeted mutation have deficits in learning and/or memory (hippocampal long-term synaptic plasticity is impaired). When treated with the melatonin receptor antagonist, luzindole, the antidepressant-like effect of the ligand is not seen. This mutant mouse strain may be useful in studies of circadian rhythm, learning and behavior.
A targeting vector containing a PGK-Neomycin cassette was used to disrupt exon 1. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric male animals were crossed to 129/sv female mice. The mice were then backcrossed to C3H/He for 8 generations before arriving at The Jackson Laboratory. The mice were crossed to C3H/HeJ (STOCK no.659) once to establish the colony.
|Allele Name||targeted mutation 1, David R Weaver|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||MT2 KO|
|Gene Symbol and Name||Mtnr1b, melatonin receptor 1B|
|Strain of Origin||129S4/SvJae|
|Molecular Note||Exon 1 was disrupted by the insertion of a PGK-neo cassette.|
|Mutations Made By|| |
David Weaver, Univ of Massachusetts Medical School
When maintaining a live colony, these mice can be bred as homozygotes.
When using the C3.129S4-Mtnr1btm1Drw/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010488 in your Materials and Methods section.