This spontaneous intragenic deletion in Mctp1 also causes reduced expression of Nr2f1 in the developing cochlea, but not retina, indicative of a disruption in the long-range regulation of Nr2f1 transcription. This strain is valuable for understanding both the function of MCTP1 and the regulation and inner ear-specific function of NR2F1.
Read More +Genetic Background | Generation |
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000664 C57BL/6J |
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Allele Type | Gene Symbol | Gene Name |
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Spontaneous (Modified regulatory region) | Mctp1 | multiple C2 domains, transmembrane 1 |
This spontaneous deletion in the sequence of Mctp1 encompasses exons 11-15, their intervening introns, 7,270 bp of intron 10-11 and 40,908 bp of intron 15-16. This deletion is expected to cause a frameshift and premature stop codon resulting in the loss of the third C2 domain and the two transmembrane helices of MCTP1. However, it is the long-range dysregulation of NR2F1 expression during inner ear development, and not Mctp1 inactivation, that is believed to be the primary cause of the outward phenotype of Mctp1dwnd mutant mice. Mice homozygous for the Mctp1 deaf wanderer mutation display moderate circling behavior due to inner ear defects, whereas Mctp1 knockout mice have normal inner ear anatomy and function. The cochlea of Mctp1dwnd mutant mice is smaller and shorter than normal, there are extra, disorganized cochlear inner hair cells near the base, an extra row of outer hair cells near the apex, the saccule is smaller than normal and fails to separate fully from the utricle, and the cochleosaccular duct is larger than normal. Auditory brainstem response analysis showed hearing impairment as early as 3 to 4 weeks of age, with thresholds 25-45 dB above those of controls.
RT-qPCR for exons 2-3 of Mctp1 in extractions from the E16.5 cochlear membranous labyrinth showed approximately 25% of normal levels of expression in deaf wanderer homozygotes. Additionally, RT-qPCR for Nr2f1 revealed a 50% reduction in expression in the cochlea of deaf wanderer homozygotes, but no change in expression in the retina, consistent with the loss of an auditory-specific enhancer region for Nr2f1 within the Mctp1 sequence deleted in deaf wanderer. Nr2f1 is approximately 1.4 Mb from Mctp1 on Chromosome 13. Mice homozygous for the Ming-Jer Tsai targeted disruption of Nr2f1, which deletes the amino terminus, DNA binding domain, and some of the ligand binding domain of NR2F1 (Tang et al, 2006), also have abnormal cochlear development with regions of supernumerary hair cells consistent with the phenotype of deaf wanderer homozygotes. However, all die neonatally with no swallowing reflex, defects in the ninth cranial ganglion, and further axonal guidance and arborization defects elsewhere. Consistent with the deaf wanderer deletion modifying Nr2f1 expression, Mctp1dwnd +/+ Nr2f1 tm1.1(KOMP)Mbp transheterozygotes have cochlear hair cell disorganization, excess inner hair cells, an extra row of outer hair cells in the apex region, malformed utricular and saccular macula, and hearing impairment consistent with that found in deaf wanderer homozygotes.
The deaf wanderer mutation arose spontaneously in the C57BL/10SnJ inbred strain at The Jackson Laboratory. This mutation was backcrossed onto the C57BL/6J background in the laboratory of Dr. Kenneth Johnson by backcross-intercross breeding and when this congenic reached generation N6 it was then maintained by sibling intercrossing. In 2011 sperm was cryopreserved from homozygous males at generation N6F11.
Allele Name | deaf wanderer |
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Allele Type | Spontaneous (Modified regulatory region) |
Allele Synonym(s) | |
Gene Symbol and Name | Mctp1, multiple C2 domains, transmembrane 1 |
Gene Synonym(s) | |
Strain of Origin | C57BL/10SnJ |
Chromosome | 13 |
Molecular Note | This 53,008 bp deletion, spanning from Chr 13: 76,815,703 bp to 76,868,709 bp deletes all of exons 11-15, 7,270 bp of flanking intron 10-11 and 40,908 bp of flanking intron 15-16, and is expected to eliminate the third C2 deomain and the two transmembrane helices, in addition to disrupting an enhancer of Nr2f1. |
A simple, PCR-based geotyping protocol is presented in Tarchini et al. 2018
When using the deaf wanderer mouse strain in a publication, please cite the originating article(s) and include JAX stock #009690 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Mctp1<dwnd> |
Frozen Mouse Embryo | B6.B10Sn-Mctp1<dwnd>/Kjn | $2595.00 |
Frozen Mouse Embryo | B6.B10Sn-Mctp1<dwnd>/Kjn | $2595.00 |
Frozen Mouse Embryo | B6.B10Sn-Mctp1<dwnd>/Kjn | $3373.50 |
Frozen Mouse Embryo | B6.B10Sn-Mctp1<dwnd>/Kjn | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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