Mice that are homozygous for this targeted mutation of Mtnr1a, melatonin receptor 1A, do not phase shift in response to melatonin injections and have sensormotor deficits and increased depressive-like behaviors. This mutant mouse strain may be useful in studies of circadian rhythm and behavior.
David R. Weaver, Univ of Massachusetts Medical School
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Mtnr1a | melatonin receptor 1A |
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical abnormalities. No gene product (protein) binding is detected in brain by autoradiographic analysis. SCN (suprachiasma nucleus) slices from homozygous mice do not exhibit suppression of neuronal firing in response to melatonin. In vitro, melatonin at higher concentrations causes phase shifts (~4hr phase advance) suggesting another receptor can influence SCN phase. Homozygous mice do not phase shift in response to melatonin injections. Overall homozygotes do not have obvious behavioral deficits, but detailed examination reveals that homozygotes have sensormotor deficits (impaired sensorimotor gating), acoustic startle/prepulse inhibition and increases in depressive-like behaviors (spend more time floating in the Porsolt test). These mice are on the C3H genetic background and carry the retinal degeneration allele Pde6brd1. This mutant mouse strain may be useful in studies of circadian rhythm and behavior.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector containing a PGK-Neomycin cassette was used to disrupt exon 1. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Heterozygotes were intercrossed to generate homozygotes. The mice were then backcrossed to C3H/He for 10 generations before arriving at The Jackson Laboratory. The mice were crossed to C3H/HeJ (Stock No. 000659) once to establish the colony.
Allele Name | targeted mutation 1, Steven M Reppert |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | MT1 KO; MT1- |
Gene Symbol and Name | Mtnr1a, melatonin receptor 1A |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 8 |
Molecular Note | Exon 1 was replaced with a neomycin selection cassette inserted by homologous recombination. The deleted region encoded the 5' untranslated region and the first cytoplasmic loop. Autoradiography showed an absence of melatonin binding in sections of homozygous mutant brains, indicating complete functional ablation. |
Mutations Made By | David Weaver, Univ of Massachusetts Medical School |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the C3.129S4(B6)-Mtnr1atm1Rep/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #009681 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Mtnr1a<tm1Rep> |
Frozen Mouse Embryo | C3.129S4(B6)-Mtnr1a<tm1Rep>/J | $2595.00 |
Frozen Mouse Embryo | C3.129S4(B6)-Mtnr1a<tm1Rep>/J | $2595.00 |
Frozen Mouse Embryo | C3.129S4(B6)-Mtnr1a<tm1Rep>/J | $3373.50 |
Frozen Mouse Embryo | C3.129S4(B6)-Mtnr1a<tm1Rep>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.