Overview

This spontaneous mutation of the dedicator of cytokinesis 7 (Dock7^m) gene or misty displays a dilute coat color, variable spotting and some abnormalities in platelets and adipose tissue.

Genetic overview

Genetic Background	Generation

Dock7^m

Allele Type	Gene Symbol	Gene Name
Spontaneous	Dock7	dedicator of cytokinesis 7

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Research Applications

Dermatology Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

The recessive misty mutation causes a mild dilution of coat color and on certain backgrounds a white tail tip often accompanied by a belly spot. Melanocytes from m/m mice have a highly dendritic shape, show deficient proliferation in culture and have much more melanin content. Fewer melanoblasts are found in primary cultures from m/m mice than from wildtype controls. Between two and five weeks of age, m/m mice are smaller than controls. At 35 days of age they are shorter, weigh 15% less on average, and have less inguinal adipose mass than controls. Misty homozygotes completely lack brown fat. Although platelet count, seratonin content and ATP content are normal, there is increased bleed time and reduced platelet AD levels in m/m homozygotes. (Woolley, 1941 and 1945; Truett et al., 1998; Sviderskaya et al., 1998.)

Development

The coat color mutation, misty (m), arose spontaneously in a DBA/J colony at The Jackson Laboratory in 1941. The single male was identified by a light color, white tail tip and white belly spot. Misty was introgressed into C57BLKS-Lepr^db to create the repulsion stock, BKS.Cg-Dock7^m +/+ Lepr^db/J. The two alleles were used to identify genotypes in the diabetes colony prior to the use of PCR genotyping. Both mutations then were backcrossed to the C57BL/6 background. In 2008, misty was bred out of the B6.Cg-Dock7^m +/+ Lepr^db/J colony.

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Woolley GW. 1941. "Misty," a new coat color dilution in the mouse, Mus musculus. Am Naturalist 75:507-508MGI: J:12159

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Genetics

Dock7^m

Allele Symbol: Dock7^m

Allele Name	misty
Allele Type	Spontaneous
Allele Synonym(s)	m
Gene Symbol and Name	Dock7, dedicator of cytokinesis 7
Gene Synonym(s)
Strain of Origin	DBA/J
Chromosome	4
Molecular Note	Crosses between mice homozygous for misty and for moonlight, which mapped to overlapping critical regions on Chr 4, demonstrated failure of the two mutations to complement one another. Once moonlight had been identified as a mutation of Dock7 (Dock7^mnlt), sequence analysis of this gene from misty mice revealed a retrotransposon LTR insertion following nucleotide 2045 (numbering from the A of the transcription initiation codon) that interrupts exon 18 and shifts the reading frame after codon 682 so that ten incorrect amino acids are incorporated into the protein before its premature termination.

Disease/Phenotype

Disease Terms

Models with phenotypic similarity to human diseases where etiology is unknown or involving genes where ortholog is unknown.

platelet storage pool deficiency

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Dermatology Research
- Color and White Spotting Defects

Genotype: Dock7^m related

Dermatology Research
- Color and White Spotting Defects

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Dock7^m/Dock7^m
involves: DBA/J

integument phenotype

diluted coat color
- coat color is not as diluted as that of homozygous dilute (Myo5a^d) or homozygous leaden (Mlph^ln) mice
- individual hairs have more cortical pigment than found in homozygous dilute or homozygous leaden mice

variable body spotting
- white tail tip with or without a white belly spot

pigmentation phenotype

diluted coat color
- coat color is not as diluted as that of homozygous dilute (Myo5a^d) or homozygous leaden (Mlph^ln) mice
- individual hairs have more cortical pigment than found in homozygous dilute or homozygous leaden mice

variable body spotting
- white tail tip with or without a white belly spot

Genotype: Dock7^m/Dock7^m
BKS.Cg-Dock7 +/+ Lepr/BomTac

mammalian phenotype

behavior/neurological phenotype
- Normal - e; anxiety-like behavior in a light-dark transfer test; and preference for social familiarity/novelty
- Normal - homozygous mutant and wild type control (C57BL/6J) mice exhibit similar locomotor activity; response in the tail-suspension test for depressive behavior; learning as measured by habituation of object exploration; working memory as measured using the Y maze; anxiety-like behavior in a light-dark transfer test; and preference for social familiarity/novelty

The following phenotype relates to a compound genotype created using this strain

Genotype: Dock7^m/Dock7^m
B6.D(Cg)-Dock7

adipose tissue phenotype

abnormal brown adipose tissue mass
- appears to be completely absent in neonatal mice

decreased white adipose tissue amount
- mice have 21% less inguinal adipose mass

growth/size/body region phenotype

decreased body length
- mutants are 8% shorter

decreased body weight
- mutants weigh 15% less

hematopoietic system phenotype

decreased platelet ADP level
- Normal - however, the platelet count, and platelet serotonin and ATP levels are normal
- platelet ADP levels are low

homeostasis/metabolism phenotype

increased bleeding time

References

Woolley GW. 1941. "Misty," a new coat color dilution in the mouse, Mus musculus. Am Naturalist 75:507-508MGI: J:12159

Additional - Dock7^m related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Dock7
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, these mice are bred as homozygotes.
- Additional Breeding and Husbandry Support
Citation
When using the B6.D2(BKS)-Dock7^m/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #009659 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Homozygous for , 1 pair minimum

Payment Terms and Conditions

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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General Terms and Conditions

Questions about Terms of Use

Licensing Information

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Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Dock7m

Allele Symbol: Dock7m

Disease Terms

Models with phenotypic similarity to human diseases where etiology is unknown or involving genes where ortholog is unknown.

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Dock7m related

Mammalian Phenotype Terms by Genotype

Genotype: Dock7m/Dock7minvolves: DBA/J

integument phenotype

pigmentation phenotype

Genotype: Dock7m/Dock7mBKS.Cg-Dock7 +/+ Lepr/BomTac

mammalian phenotype

Genotype: Dock7m/Dock7mB6.D(Cg)-Dock7

adipose tissue phenotype

growth/size/body region phenotype

hematopoietic system phenotype

homeostasis/metabolism phenotype

References

Additional - Dock7m related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Dock7^m

Allele Symbol: Dock7^m

Genotype: Dock7^m related

Genotype: Dock7^m/Dock7^m
involves: DBA/J

Genotype: Dock7^m/Dock7^m
BKS.Cg-Dock7 +/+ Lepr/BomTac

Genotype: Dock7^m/Dock7^m
B6.D(Cg)-Dock7

Additional - Dock7^m related