These mice possess loxP sites on either side of exon 2 of the Dag1 (dystroglycan 1) gene. When bred to mice with a cre recombinase gene under the control of a promoter of interest, exon 2 of the targeted gene is deleted in the tissue of interest.
Kevin Campbell, University of Iowa
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Dag1 | dystroglycan 1 |
These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene under the control of a promoter of interest, exon 2 of the targeted gene is deleted in the tissue of interest.
A targeting vector was used to introduce a loxP site downstream of exon 2 and insert a floxed neo cassette in the upstream intron. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Resultant mice on an unknown background (probable 129-C57BL/6/J mix) were crossed with a Myl2tm1(cre)Krc strain (myosin, light polypeptide 2, regulatory, cardiac, slow promoter driving cre). The neomycin cassette was deleted, leaving a loxP site in intron 1 and another 3' to the 3' UTR. The mice were backcrossed five times to C57BL/6J to remove the cre allele.
Allele Name | targeted mutation 2.1, Kevin P Campbell |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | DAG1lox; T30mN |
Gene Symbol and Name | Dag1, dystroglycan 1 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 9 |
Molecular Note | A targeting vector was used to introduce a loxP site downstream of exon 2 and insert a floxed neo cassette in the upstream intron. Cre mediated recombination removed the neomycin cassette, leaving a loxP site in intron 1 and another 3' to the 3' UTR. |
Mutations Made By | Kevin Campbell, University of Iowa |
When maintained as a live colony, homozygotes may be bred.
When using the B6.129(Cg)-Dag1tm2.1Kcam/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #009652 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Dag1<tm2.1Kcam> |
Frozen Mouse Embryo | B6.129(Cg)-Dag1<tm2.1Kcam>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129(Cg)-Dag1<tm2.1Kcam>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129(Cg)-Dag1<tm2.1Kcam>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129(Cg)-Dag1<tm2.1Kcam>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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