B6.129(Cg)-Dag1^tm2.1Kcam/J

Stock number: 009652
Research areas: Mouse/Human Gene Homologs, Neurobiology Research, Research Tools

Description

These mice possess loxP sites on either side of exon 2 of the Dag1 (dystroglycan 1) gene. When bred to mice with a cre recombinase gene under the control of a promoter of interest, exon 2 of the targeted gene is deleted in the tissue of interest.

Detailed description

These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene under the control of a promoter of interest, exon 2 of the targeted gene is deleted in the tissue of interest.

Development

A targeting vector was used to introduce a loxP site downstream of exon 2 and insert a floxed neo cassette in the upstream intron. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl⁺-derived R1 embryonic stem (ES) cells. Resultant mice on an unknown background (probable 129-C57BL/6/J mix) were crossed with a Myl2^tm1(cre)Krc strain (myosin, light polypeptide 2, regulatory, cardiac, slow promoter driving cre). The neomycin cassette was deleted, leaving a loxP site in intron 1 and another 3' to the 3' UTR. The mice were backcrossed five times to C57BL/6J to remove the cre allele.

Allele

Symbol: Dag1^tm2.1Kcam
Name: targeted mutation 2.1, Kevin P Campbell
MGI accession ID: MGI:3841383
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Dag1
Marker name: dystroglycan 1
Chromosome: 9
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A targeting vector was used to introduce a loxP site downstream of exon 2 and insert a floxed neo cassette in the upstream intron. Cre mediated recombination removed the neomycin cassette, leaving a loxP site in intron 1 and another 3' to the 3' UTR.