Mice that are homozygous for this targeted mutation of Vip (vasoactive intestinal polypeptide) exhibit impaired circadian rhythm generation, muscle weakness, increased induced airway hyperresponsiveness/inflammation, gastrointestinal tract abnormalities. Male homozygotes exhibit moderate right ventricular hypertension and hypertrophy, reduced serum testosterone and follicle-stimulating hormone levels. All offspring of heterozygous females exhibit developmental delays such as reduced body weight and impaired social behavior. This mutant mouse strain may be useful in studies of embryonic and neonatal development, circadian rhythm, airway hyperresponsiveness and inflammation(asthma), gastrointestinal innervation and motility and idiopathic pulmonary arterial hypertension.
James Waschek, UCLA
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Vip | vasoactive intestinal polypeptide |
Mice that are heterozygous for the targeted mutation are viable and fertile. No gene product (protein) is detected by immunohistochemical analysis of homozygotes. Homozygous females are subfertile, 15-20% of homozygous females can have litters. Homozygotes exhibit impaired circadian rhythm generation, muscle weakness, increased motor activity and increased induced airway hyperresponsiveness and inflammation. In constant darkness, homozygotes initially display shortened circadian period. Some homozygotes develop arrhythmic circadian periods after extended darkness. Heterozygotes also display abnormal circadian rhythm. Homozygotes have both gross and microscopic anatomical abnormalities of the gastrointestinal tract. 10-15% of homozygotes die of stenosis of the gut before one year of age. Male homozygotes exhibit moderate right ventricular (RV) hypertension, RV hypertrophy, thickened and remodeled pulmonary arteries, perivascular inflammation of smaller pulmonary vessels and airways, reduced serum testosterone levels, and reduced serum follicle-stimulating hormone (FSH) levels. Although male homozygotes had early testicular degeneration, age-dependent degeneration is slowed. All offspring of heterozygous females exhibit developmental delays such as reduced body weight and impaired social behavior. This mutant mouse strain may be useful in studies of embryonic and neonatal development, circadian rhythm, airway hyperresponsiveness and inflammation (asthma), gastrointestinal innervation and motility and idiopathic pulmonary arterial hypertension.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 4. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting male chimeric animals were crossed to C57BL/6 female mice, and then backcrossed to C57BL/6J (see SNP notes) for 12 generations.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
Allele Name | targeted mutation 1, Christopher S Colwell |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | VIP KO; VIP/PHI-; VIP- |
Gene Symbol and Name | Vip, vasoactive intestinal polypeptide |
Gene Synonym(s) | |
Strain of Origin | 129/Sv |
Chromosome | 10 |
Molecular Note | A neomycin resistance gene was inserted in reverse orientation into exon 4. PCR confirmed recombination in mutant mice. Immunohistochemistry confirmed the loss of protein in homozygotes. |
Mutations Made By | James Waschek, UCLA |
When maintaining a live colony, these mice are bred as heterozygous females and homozygous males. Homozygous females are subfertile; 15-20% of homozygous females can have litters .
When using the B6.129S4-Viptm1Clw/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #009640 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Vip<tm1Clw> |
Frozen Mouse Embryo | B6.129S4-Vip<tm1Clw>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S4-Vip<tm1Clw>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S4-Vip<tm1Clw>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S4-Vip<tm1Clw>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.