These Dual-glo transgenic mice express both firefly luciferase (luc) under the control of the human GFAP (glial fibrillary acidic protein) promoter and Renilla luciferase under the control of the 0.5kb human GAPDH (glyceraldehyde-3-phosphate dehydrogenase) promoter. GFAP-fLuc transgene expression is highest in brain, with lower levels detected in heart and no detectable expression in kidney, muscle, lung and liver. The firefly luciferase protein co-localizes with GFAP in astrocytes after kainic acid induced injury. GAPDH-RLuc transgene expression is highest in brain and heart, with lower levels detected in kidney, muscle, lung and liver. Interindividual variability in firefly luciferase expression is reduced with normalization of the GFAP-fLuc signal to the GAPDH-RLuc signal. This mutant mouse strain may be useful in studies of Alexander disease, astrocyte biology, and gliosis.
Dr. Albee Messing, University of Wisconsin-Madison
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
These Dual-glo transgenic mice express both firefly luciferase (luc) under the control of the human GFAP (glial fibrillary acidic protein) promoter and Renilla luciferase under the control of the 0.5kb human GAPDH (glyceraldehyde-3-phosphate dehydrogenase) promoter. GFAP-fLuc transgene expression is highest in brain, with lower levels detected in heart and no detectable expression in kidney, muscle, lung and liver. The firefly luciferase protein co-localizes with GFAP in astrocytes after kainic acid induced injury. GAPDH-RLuc transgene expression is highest in brain and heart, with lower levels detected in kidney, muscle, lung and liver. Interindividual variability in firefly luciferase expression is reduced with normalization of the GFAP-fLuc signal to the GAPDH-RLuc signal. Retinal expression of luciferase is increased due to photoreceptor degeneration for mice on the FVB background and the homozygous presence of the retinal degeneration 1, Pde6brd1 (rd) mutation. Firefly luciferase and GFAP expression increases in response to chemical (Kainic acid) induced injury. Mice that are hemizygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of Alexander disease, astrocyte biology, and gliosis.
To generate the Dual-glo transgenic line, two transgenic constructs were co-injected into FVB/N fertilized oocytes. The first construct (GFAP-fLuc) included the firefly luciferase gene under the control of the 2.2kb human GFAP, glial fibrillary acidic protein promoter. The other construct (GAPDH-RLuc) consisted of the Renilla luciferase gene under the control of the 0.5kb human GAPDH, glyceraldehyde-3-phosphate dehydrogenase promoter. Founder line 172.9 was subsequently established. The mice were backcrossed to FVB/N for 10 generations before arriving at The Jackson Laboratory. The mice were crossed to FVB/NJ once to establish the colony.
Expressed Gene | luc, luciferase, firefly |
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Expressed Gene | rluc, Renilla luciferase, sea pansy |
Site of Expression |
Allele Name | transgene insertion 172.9, Albee Messing |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | Dual-Glo; GFAP-fluc and GADPH-Rluc; GFAP-luc and GADPH-Rluc; Tg172-9 |
Gene Symbol and Name | Tg(GFAP-luc,GAPDH-rluc)172.9Mes, transgene insertion 172.9, Albee Messing |
Gene Synonym(s) | |
Promoter | GFAP, glial fibrillary acidic protein, human |
Promoter | GAPDH, glyceraldehyde-3-phosphate dehydrogenase, human |
Expressed Gene | luc, luciferase, firefly |
Expressed Gene | rluc, Renilla luciferase, sea pansy |
Strain of Origin | (FVB x C57BL/6)F1 |
Chromosome | UN |
Molecular Note | Two transgenic constructs were co-injected into fertilized oocytes. The first construct (GFAP-fLuc) included the firefly luciferase gene under the control of the 2.2kb human GFAP, glial fibrillary acidic protein promoter. The other construct (GAPDH-RLuc) consisted of the Renilla luciferase gene under the control of the 0.5kb human GAPDH, glyceraldehyde-3-phosphate dehydrogenase promoter. Founder line 172.9 was subsequently established. |
When maintaining a live colony, these mice can be bred as hemizygotes.
When using the FVB-Tg(GFAP-luc,GAPDH-rluc)172.9Mes/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #009638 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non carrier for Tg(GFAP-luc,GAPDH-rluc)172.9Mes |
Frozen Mouse Embryo | FVB-Tg(GFAP-luc GAPDH-rluc)172.9Mes/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(GFAP-luc GAPDH-rluc)172.9Mes/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(GFAP-luc GAPDH-rluc)172.9Mes/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | FVB-Tg(GFAP-luc GAPDH-rluc)172.9Mes/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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