These TgNL1.6 mice harbor have expression of a hemagglutinin-tagged mouse neuroligin 1 (Nlgn1 or NL1) gene driven by the murine Thy1.2 expression cassette. TgNL1.6 mice manifest significant deficits in memory acquisition, impairments in the induction of long-term potentiation, altered spine morphology, and enhanced excitatory synaptic transmission that lead to learning deficits. These transgenic mice may be useful in neurobiological studies of neuroligin adhesion molecules in excitatory/inhibitory synapse maturation and function, memory formation and synaptic plasticity.
Rochelle M Hines, University of British Columbia
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Mice hemizygous for the TgNL1 transgene are viable and fertile. The TgNL1 transgene encodes a hemagglutinin-tagged mouse neuroligin 1 (Nlgn1 or NL1) gene driven by the murine Thy1.2 expression cassette. HA-NL1 transcript and protein is expressed in brain and to a lesser extend in spinal cord, with highest levels of expression in the cortex and hippocampus, intermediate in the striatum and lowest in the cerebellum and olfactory bulb. TgNL1.6 mice have moderate to high levels of HA-NL1 expression (approximately two-fold greater than wildtype NL1). This overexpression results in significant deficits in memory acquisition, impairments in the induction of long-term potentiation, altered spine morphology, and enhanced excitatory synaptic transmission that lead to learning deficits. These TgNL1.6 mice may be useful in neurobiological studies of neuroligin adhesion molecules in excitatory/inhibitory synapse maturation and function, memory formation and synaptic plasticity.
The TgNL1 transgene was designed with a modified regulatory region of the "murine thy1.2 gene" (extending from the promoter to the intron following exon 4, excluding exon 3 and its flanking introns) upstream of a hemagglutinin-tagged mouse neuroligin 1 (Nlgn1 or NL1) cDNA. This transgene was injected into (C57BL/6xCBA)F1 cells. Transgenic founders were bred with C57BL/6 and mice from founder line 6 (TgNL1.6) were found to have approximately two-fold increase in NL1 expression. TgNL1.6 mice were then bred to C57BL/6J mice for at approximately nine generations prior to arrival at The Jackson Laboratory. Upon arrival, TgNL1.6 mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6 genetic background.
Expressed Gene | Nlgn1, neuroligin 1, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 6, Rochelle Hines |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | TgNL1; TgNL1.6 |
Gene Symbol and Name | Tg(Thy1-Nlgn1)6Hnes, transgene insertion 6, Rochelle Hines |
Gene Synonym(s) | |
Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory |
Expressed Gene | Nlgn1, neuroligin 1, mouse, laboratory |
Strain of Origin | (C57BL/6 x CBA)F1 |
Chromosome | UN |
Molecular Note | The TgNL1 transgene was designed with a modified regulatory region of the "murine thy1.2 gene" (extending from the promoter to the intron following exon 4, excluding exon 3 and its flanking introns) upstream of a hemagglutinin-tagged mouse neuroligin 1 (Nlgn1 or NL1) cDNA. This transgene was injected into (C57BL/6xCBA)F1 cells. Transgenic founders were bred with C57BL/6 and mice from founder line 6 (TgNL1.6) were found to have approximately two-fold increase in NL1 expression. |
Mutations Made By | Rochelle Hines, University of British Columbia |
When maintaining a live colony, hemizygous mice may be bred together, to wildtype (noncarrier) siblings or to C57BL/6J inbred mice (Stock No. 000664).
When using the B6.Cg-Tg(Thy1-Nlgn1)6Hnes/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #009611 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non carrier for Tg(Thy1-Nlgn1)6Hnes |
Frozen Mouse Embryo | B6.Cg-Tg(Thy1-Nlgn1)6Hnes/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Thy1-Nlgn1)6Hnes/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Thy1-Nlgn1)6Hnes/J | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Thy1-Nlgn1)6Hnes/J | $3373.50 |
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