Hemizygous mice are viable and fertile with no reported phenotypic abnormalities from the insertion of five full-length human AAVS1 sites (adeno-associated virus integration site 1) at a single chromosomal locus. The AAVS1 loci within the transgene allow site-specific integration of hybrid vectors containing AAV inverted terminal repeat-flanked genes of interest.
Xandra O Breakefield, Massachusetts General Hospital-East
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
Hemizygous mice are viable and fertile with no reported phenotypic abnormalities from the insertion of multiple full-length human AAVS1 sites (adeno-associated virus integration site 1) at a single chromosomal locus. The multiple (approximately five) AAVS1 loci within the transgene allow site-specific integration of hybrid vectors containing AAV inverted terminal repeat-flanked genes of interest.
Adeno-associated virus (AAV) is a nonpathogenic parvovirus with a single-stranded DNA genome (4.7 kb) containing two genes (rep and cap) flanked by 145-base inverted terminal repeats (ITRs). Efficient replication of the AAV genome occurs in the presence of a helper virus, either adenovirus or herpes simplex virus. In the absence of a helper virus, ITRs and Rep proteins are sufficient to mediate the integration of AAV sequences into a specific AAVS1 site in the host cell genome and thereby establish a latent infection. In addition, low levels of helper-independent AAV replication can also occur in cells that are chemically synchronized to the S phase or exposed to toxic conditions, including UV irradiation, heat, or carcinogens. Because of this, the AAVS1 loci within the transgene are a platform for site-specific integration of AAV ITR-flanked hybrid vectors that contain genes of interest.
An 8.2 kb human genomic fragment containing the entire adeno-associated virus integration site 1 (AAVS1) locus was isolated after EcoRI digestion of plasmid pRIA-N3. This transgene was microinjected into fertilized oocytes of B6C3F1 mice. Male founders were bred with wildtype C3H female mice (C3H substrain not known). Transgenic founder line A1 was established and found to have approximately 5 copies of the transgene integrated at a single locus. The AAVS1 transgenic mice were maintained by breeding together for many generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred with C3HeB/FeJ inbred mice (Stock No. 000658) for at least one generation to establish the colony.
Expressed Gene | AAVS1, adeno-associated virus integration site 1, human |
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Site of Expression |
Allele Name | transgene insertion A1, Xandra Breakefield |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | AAVS1 transgenic mice (founder line A1) |
Gene Symbol and Name | Tg(AAVS1)A1Xob, transgene insertion A1, Xandra Breakefield |
Gene Synonym(s) | |
Promoter | AAVS1, adeno-associated virus integration site 1, human |
Expressed Gene | AAVS1, adeno-associated virus integration site 1, human |
Strain of Origin | (C57BL/6 x C3H)F1 |
Chromosome | UN |
Molecular Note | An 8.2 kb human genomic fragment containing the entire adeno-associated virus integration site 1 (AAVS1) locus was isolated after EcoRI digestion of plasmid pRIA-N3. Transgenic founder line A1 was established and found to have approximately 5 copies of the transgene integrated at a single locus. |
Mutations Made By | Xandra Breakefield, Massachusetts General Hospital-East |
When maintaining a live colony, hemizygous mice may be bred to noncarrier (wildtype) siblings. In an emergency, transgenic mice may be bred to C3HeB/FeJ inbred mice (Stock No. 000658).
When using the C3;B6-Tg(AAVS1)A1Xob/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #009601 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous for Tg(AAVS1)A1Xob |
Frozen Mouse Embryo | C3;B6-Tg(AAVS1)A1Xob/J | $2595.00 |
Frozen Mouse Embryo | C3;B6-Tg(AAVS1)A1Xob/J | $2595.00 |
Frozen Mouse Embryo | C3;B6-Tg(AAVS1)A1Xob/J | $3373.50 |
Frozen Mouse Embryo | C3;B6-Tg(AAVS1)A1Xob/J | $3373.50 |
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