These floxed mice harbor loxP sites flanking exon 2 of the TNF-α converting enzyme (TACE, also referred to as ADAM17 [a disintegrin and metallopeptidase domain 17]) locus. As the proinflammatory cytokine TNF-α and other other cell receptors are synthesized as membrane-bound precursors that need to be proteolytically released by functional TACE, these Taceflox mice may be useful in generating conditional mutations for studying TNF-sheddase function and TNF-related autoimmune diseases.
Carl P Blobel, Hospital for Special Surgery-Weill Med
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Adam17 | a disintegrin and metallopeptidase domain 17 |
Mice homozygous for this Taceflox allele are viable and fertile, with loxP sites flanking exon 2 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have the floxed sequences deleted in the cre-expressing tissue producing a null allele.
As the proinflammatory cytokine TNF-α and other other cell receptors are synthesized as membrane-bound precursors that need to be proteolytically released by functional TNF-α converting enzyme (TACE or Adam17 [a disintegrin and metallopeptidase domain 17]), these Taceflox mutant mice may be useful in generating conditional mutations for studying TNF-sheddase function, TNF-related autoimmune diseases.
A targeting vector was designed to insert a loxP site and frt-flanked PGK-neo cassette upstream of exon 2, and a second loxP site downstream of exon 2 of the targeted gene. The donating investigator reports that the construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with C57BL/6 females to establish the colony. The mutant mice (C57BL/6;129P2/OlaHsd genetic background) were then bred to mice expressing Flp recombinase (B6;SJL genetic background; see Stock No. 003800) to remove the frt-flanked neo cassette. The resulting Taceflox mice (with loxP site and single frt site remaining upstream of exon 2, and a second loxP site downstream of exon 2) were maintained on this mixed genetic background for many generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 1.2, Carl P Blobel |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | |
Gene Symbol and Name | Adam17, a disintegrin and metallopeptidase domain 17 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 12 |
Molecular Note | A targeting vector was designed to insert a loxP site and frt-flanked PGK-neo cassette upstream of exon 2, and a second loxP site downstream of exon 2 of the targeted gene. The resulting mutant mice were then bred to mice expressing Flp recombinase to remove the frt-flanked neo cassette. |
Mutations Made By | Carl Blobel, Hospital for Special Surgery-Weill Med |
When using the Taceflox mouse strain in a publication, please cite the originating article(s) and include JAX stock #009597 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous for Adam17<tm1.2Bbl>, 1 pair minimum |
Frozen Mouse Embryo | STOCK Adam17<tm1.2Bbl>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | STOCK Adam17<tm1.2Bbl>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | STOCK Adam17<tm1.2Bbl>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | STOCK Adam17<tm1.2Bbl>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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