These floxed mice harbor loxP sites flanking exon 2 of the TNF-α converting enzyme (TACE, also referred to as ADAM17 [a disintegrin and metallopeptidase domain 17]) locus. As the proinflammatory cytokine TNF-α and other other cell receptors are synthesized as membrane-bound precursors that need to be proteolytically released by functional TACE, these Taceflox mice may be useful in generating conditional mutations for studying TNF-sheddase function and TNF-related autoimmune diseases.
Carl P Blobel, Hospital for Special Surgery-Weill Med
Mice homozygous for this Taceflox allele are viable and fertile, with loxP sites flanking exon 2 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have the floxed sequences deleted in the cre-expressing tissue producing a null allele.
As the proinflammatory cytokine TNF-α and other other cell receptors are synthesized as membrane-bound precursors that need to be proteolytically released by functional TNF-α converting enzyme (TACE or Adam17 [a disintegrin and metallopeptidase domain 17]), these Taceflox mutant mice may be useful in generating conditional mutations for studying TNF-sheddase function, TNF-related autoimmune diseases.
A targeting vector was designed to insert a loxP site and frt-flanked PGK-neo cassette upstream of exon 2, and a second loxP site downstream of exon 2 of the targeted gene. The donating investigator reports that the construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with C57BL/6 females to establish the colony. The mutant mice (C57BL/6;129P2/OlaHsd genetic background) were then bred to mice expressing Flp recombinase (B6;SJL genetic background; see Stock No. 003800) to remove the frt-flanked neo cassette. The resulting Taceflox mice (with loxP site and single frt site remaining upstream of exon 2, and a second loxP site downstream of exon 2) were maintained on this mixed genetic background for many generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1.2, Carl P Blobel|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Adam17, a disintegrin and metallopeptidase domain 17|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||A targeting vector was designed to insert a loxP site and frt-flanked PGK-neo cassette upstream of exon 2, and a second loxP site downstream of exon 2 of the targeted gene. The resulting mutant mice were then bred to mice expressing Flp recombinase to remove the frt-flanked neo cassette.|
|Mutations Made By|| |
Carl Blobel, Hospital for Special Surgery-Weill Med
When using the Taceflox mouse strain in a publication, please cite the originating article(s) and include JAX stock #009597 in your Materials and Methods section.
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