STOCK Adam17^tm1.2Bbl/J

Stock number: 009597
Product name: Tace^flox
Research areas: Cancer Research, Cell Biology Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These floxed mice harbor loxP sites flanking exon 2 of the TNF-α converting enzyme (TACE, also referred to as ADAM17 [a disintegrin and metallopeptidase domain 17]) locus. As the proinflammatory cytokine TNF-α and other other cell receptors are synthesized as membrane-bound precursors that need to be proteolytically released by functional TACE, these Tace^flox mice may be useful in generating conditional mutations for studying TNF-sheddase function and TNF-related autoimmune diseases.

Detailed description

Mice homozygous for this Tace^flox allele are viable and fertile, with loxP sites flanking exon 2 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have the floxed sequences deleted in the cre-expressing tissue producing a null allele. As the proinflammatory cytokine TNF-α and other other cell receptors are synthesized as membrane-bound precursors that need to be proteolytically released by functional TNF-α converting enzyme (TACE or Adam17 [a disintegrin and metallopeptidase domain 17]), these Tace^flox mutant mice may be useful in generating conditional mutations for studying TNF-sheddase function, TNF-related autoimmune diseases.

Development

A targeting vector was designed to insert a loxP site and frt-flanked PGK-neo cassette upstream of exon 2, and a second loxP site downstream of exon 2 of the targeted gene. The donating investigator reports that the construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with C57BL/6 females to establish the colony. The mutant mice (C57BL/6;129P2/OlaHsd genetic background) were then bred to mice expressing Flp recombinase (B6;SJL genetic background; see Stock No. 003800) to remove the frt-flanked neo cassette. The resulting Tace^flox mice (with loxP site and single frt site remaining upstream of exon 2, and a second loxP site downstream of exon 2) were maintained on this mixed genetic background for many generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Adam17^tm1.2Bbl
Name: targeted mutation 1.2, Carl P Blobel
MGI accession ID: MGI:4354847
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Adam17
Marker name: a disintegrin and metallopeptidase domain 17
Chromosome: 12
Strain of origin: 129P2/OlaHsd
Molecular note: A targeting vector was designed to insert a loxP site and frt-flanked PGK-neo cassette upstream of exon 2, and a second loxP site downstream of exon 2 of the targeted gene. The resulting mutant mice were then bred to mice expressing Flp recombinase to remove the frt-flanked neo cassette.