These mice have the Ple183-EGFP transgene targeted as a single copy "knockin" into the upstream region of the Hprt locus on the X chromosome. The promoter/regulatory regions of the human S100B gene direct expression of EGFP.
Elizabeth M Simpson, Centre for Molecular Medicine & Therapeutics, University of British Columbia
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter) | Hprt | hypoxanthine guanine phosphoribosyl transferase |
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | EGFP expression is directed to Bergmann glial cells in the cerebellum. |
Allele Name | targeted mutation 33, Elizabeth M Simpson |
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Allele Type | Targeted (Reporter) |
Allele Synonym(s) | Hprttm33(mEMS2742)Ems; Ple183; S100B-A-EGFP |
Gene Symbol and Name | Hprt, hypoxanthine guanine phosphoribosyl transferase |
Gene Synonym(s) | |
Promoter | S100B, S100 calcium binding protein B, human |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | EGFP expression is directed to Bergmann glial cells in the cerebellum. |
Strain of Origin | (B6.129P2-Hprtb-m3/J x 129S-Gt(ROSA)26Sortm1Sor/J)F1 |
Chromosome | X |
General Note | Germ line transmission of mutant cell line mEMS2742 has been established. |
Molecular Note | The Ple183-EGFP transgene (pEMS1382) was designed with the 1384 bp Ple183 minipromoter (S100B-A; derived from a subsection of the promoter from the human S100 calcium binding protein B (S100B) gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. The promoter/regulatory regions of the human S100 calcium binding protein B (S100B) gene directs expression of enhanced green fluorescent protein (EGFP) to Bergmann glial cells in the cerebellum. No EGFP signal above background level could be observed in other regions of the brain, such as cortex and brainstem. |
Mutations Made By | Elizabeth Simpson, Centre for Molecular Medicine & Therapeutics, University of British Columbia |
The donating investigator recommends maintaining this strain by breeding heterozygous females with C57BL/6J inbred males.
When using the B6.129P2(Cg)-Hprttm33(Ple183-EGFP)Ems/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32934 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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