B6.129P2(Cg)-Hprt^{tm33(Ple183-EGFP)Ems}/Mmjax

Stock number: 009596
Strain synonyms: B6.129P2(Cg)-Hprt
Research areas: Neurobiology Research, Research Tools

Description

These mice have the Ple183-EGFP transgene targeted as a single copy "knockin" into the upstream region of the Hprt locus on the X chromosome. The promoter/regulatory regions of the human S100B gene direct expression of EGFP.

Allele

Symbol: Hprt^{tm33(Ple183-EGFP)Ems}
Name: targeted mutation 33, Elizabeth M Simpson
MGI accession ID: MGI:4834776
Generation method: Targeted
Attributes: Reporter
Marker symbol: Hprt
Marker name: hypoxanthine guanine phosphoribosyl transferase
Chromosome: X
Strain of origin: (B6.129P2-Hprt^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: EGFP expression is directed to Bergmann glial cells in the cerebellum.
Molecular note: The Ple183-EGFP transgene (pEMS1382) was designed with the 1384 bp Ple183 minipromoter (S100B-A; derived from a subsection of the promoter from the human S100 calcium binding protein B (S100B) gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome.

The promoter/regulatory regions of the human S100 calcium binding protein B (S100B) gene directs expression of enhanced green fluorescent protein (EGFP) to Bergmann glial cells in the cerebellum. No EGFP signal above background level could be observed in other regions of the brain, such as cortex and brainstem.
General note: Germ line transmission of mutant cell line mEMS2742 has been established.