| Allele Name | targeted mutation 33, Elizabeth M Simpson |
| Allele Type | Targeted (Reporter) |
| Allele Synonym(s) | Hprttm33(mEMS2742)Ems; Ple183; S100B-A-EGFP |
| Gene Symbol and Name | Hprt, hypoxanthine guanine phosphoribosyl transferase |
| Gene Synonym(s) | |
| Promoter | S100B, S100 calcium binding protein B, human |
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| Expressed Gene | GFP, Green Fluorescent Protein, |
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| Site of Expression | EGFP expression is directed to Bergmann glial cells in the cerebellum. |
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| Strain of Origin | (B6.129P2-Hprtb-m3/J x 129S-Gt(ROSA)26Sortm1Sor/J)F1 |
| Chromosome | X |
| General Note | Germ line transmission of mutant cell line mEMS2742 has been established. |
| Molecular Note | The Ple183-EGFP transgene (pEMS1382) was designed with the 1384 bp Ple183 minipromoter (S100B-A; derived from a subsection of the promoter from the human S100 calcium binding protein B (S100B) gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. The promoter/regulatory regions of the human S100 calcium binding protein B (S100B) gene directs expression of enhanced green fluorescent protein (EGFP) to Bergmann glial cells in the cerebellum. No EGFP signal above background level could be observed in other regions of the brain, such as cortex and brainstem. |
| Mutations Made By | Elizabeth Simpson, Centre for Molecular Medicine & Therapeutics, University of British Columbia |
