In this strain, the targeted mutation replaces exon 5 and part of exon 6 of the c-abl concogene 1, non-receptor tyrosine kinase gene, Abl1 with a neomycin resistance (neo) cassette, abolishing gene function. These mice may be useful for studying cell proliferation, survival and migration, as well as lymphopoesis and hematopoiesis.
Stephen P. Goff, Columbia University
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Abl1 | c-abl oncogene 1, non-receptor tyrosine kinase |
In this strain, the targeted mutation replaces exon 5 and part of exon 6 of the c-abl concogene 1, non-receptor tyrosine kinase gene, Abl1 with a neomycin resistance (neo) cassette, abolishing gene function. Mice heterozygous for this allele are viable, fertile, and normal in size. Homozygous c-Abl2 mice exhibit no abnormal phenotype before birth. After birth 95% of all homozygotes become runted and 85% develop lymphopenia. During the first week of age fatty vacuoles are evident in hepatocytes and some hepatic degeneration is evident. Spleens are severely atrophied, as are thymuses which show a severe deficiency in thymocytes. The few remaining thymocytes are only single positive CD4 or CD8 T cells. During the first 3 weeks of life 7% of the homozygotes are runted and died and 6% disappear, leaving approximately 7% of homozygous mutants. The few that survived to 3 or 4 months of age exhibited megaesophagus, anal prolapsed, aspiration pneumonia, and signs of infection in the nasal passages and ears. Mutants weighing 50% of the weight of controls had 50% fewer bone marrow cells, whereas mutant thymocytes decreased 20- to 500-fold in number. These mice may be useful for studying cell proliferation, survival and migration, as well as lymphopoesis and hematopoiesis.
A targeting vector was designed to replace exon 5 and part of exon 6 of the c-abl concogene 1, non-receptor tyrosine kinase gene, Abl1 with a neomycin resistance (neo) cassette. The construct was electroporated into 129S/SvEv-Gpic-derived CCE embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric males were bred to C57BL/6 females to establish a colony. These c-Abl2 mice were backcrossed to C57BL/6 for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 1, Richard C Mulligan |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | abl-; Abl2; ablm2; c-Abl-; c-abl2 |
Gene Symbol and Name | Abl1, c-abl oncogene 1, non-receptor tyrosine kinase |
Gene Synonym(s) | |
Strain of Origin | 129S/SvEv-Gpi1c |
Chromosome | 2 |
Molecular Note | A genomic fragment containing exon 5 and part of exon 6 was replaced by a neomycin resistance cassette. These sequences encode the N-terminal part of the tyrosine kinase domain, including the nucleotide and ATP binding sites. |
Mutations Made By | Dr. Richard Mulligan, Harvard Medical School |
When maintained as a live colony, heterozygotes may be bred. Viability of homozygotes is reduced and surviving males have reduced fertility.
When using the B6.129S-Abl1tm1Mlg/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #009417 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Abl1<tm1Mlg> |
Frozen Mouse Embryo | B6.129S-Abl1<tm1Mlg>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S-Abl1<tm1Mlg>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S-Abl1<tm1Mlg>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S-Abl1<tm1Mlg>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.