Preliminary information suggests these Pstpip1 (proline-serine-threonine phosphatase-interacting protein 1) targeted mice have various defects in immune cells, predominantly T lymphocytes which appear to be hyperresponsive to antigen receptor stimulation.
Stephen P. Goff, Columbia University
Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. Studies of spleen, thymus and embryonic fibroblasts indicate that expression is eliminated. Preliminary information suggests these mice have various defects in immune cells, predominantly T lymphocytes which appear to be hyperresponsive to antigen receptor stimulation.
Exon 1 was replaced by a loxP-flanked neomycin resistance cassette placed in the same transcriptional orientation as the targeted gene. A 129/SvEv-derived embryonic stem cell line was used to create the mutation. This strain was backcrossed to C57BL/6 (see SNP note below) nine times by the donating laboratory.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 4 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
|Allele Name||targeted mutation 1, Stephen P Goff|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Pstpip1, proline-serine-threonine phosphatase-interacting protein 1|
|Strain of Origin||129S/SvEv|
|Molecular Note||Exon 1 was replaced by a loxP-flanked neomycin resistance cassette placed in the same transcriptional orientation as the targeted gene.|
|Mutations Made By|| |
Stephen Goff, Columbia University
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the B6.129S-Pstpip1tm1Spg/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #009411 in your Materials and Methods section.