B6.129S-Pstpip1^tm1Spg/J

Stock number: 009411
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

Preliminary information suggests these Pstpip1 (proline-serine-threonine phosphatase-interacting protein 1) targeted mice have various defects in immune cells, predominantly T lymphocytes which appear to be hyperresponsive to antigen receptor stimulation.

Detailed description

Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. Studies of spleen, thymus and embryonic fibroblasts indicate that expression is eliminated. Preliminary information suggests these mice have various defects in immune cells, predominantly T lymphocytes which appear to be hyperresponsive to antigen receptor stimulation.

Development

Exon 1 was replaced by a loxP-flanked neomycin resistance cassette placed in the same transcriptional orientation as the targeted gene. A 129/SvEv-derived embryonic stem cell line was used to create the mutation. This strain was backcrossed to C57BL/6 (see SNP note below) nine times by the donating laboratory.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 4 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Allele

Symbol: Pstpip1^tm1Spg
Name: targeted mutation 1, Stephen P Goff
MGI accession ID: MGI:4361584
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Pstpip1
Marker name: proline-serine-threonine phosphatase-interacting protein 1
Marker synonyms: CD2BP1; CD2BP1L; CD2BP1S; def-2; H-PIP; PAPAS; PSTPIP
Chromosome: 9
Strain of origin: 129S/SvEv
Molecular note: Exon 1 was replaced by a loxP-flanked neomycin resistance cassette placed in the same transcriptional orientation as the targeted gene.