B6;129S1-Osr2^tm2(cre)Jian/J

Stock number: 009388
Research areas: Research Tools

Description

These Osr2-IresCre (or Osr2^IresCre) mice harbor an IRES-Cre inserted into the 3' UTR of Osr2 (odd-skipped related 2 (Drosophila)) locus. When bred with mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequence primarily in developing palate mesenchyme and metanephric mesenchyme-derived glomeruli tissues in the offspring. These mice may be useful for generating conditional mutations for studying developmental biology (palate and kidney development).

Detailed description

Mice homozygous for the Osr2-IresCre (or Osr2^IresCre) allele are viable and fertile, with an IRES-Cre bicistronic expression cassette inserted into the 3' UTR of the targeted locus. As such, cre expression is directed by the endogenous promoter/enhancer regions primarily to developing palate mesenchyme and metanephric mesenchyme-derived glomeruli tissues (but not other epithelial and mesenchymal tissues in the developing metanephric kidney). Some ectopic cre activity is reported (particularly in the central nervous system). When bred with mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequence in these tissues in the offspring. These Osr2-IresCre mice may be useful for generating conditional mutations for studying developmental biology (palate and kidney development).

View cre expression characterization.

Development

A targeting vector was designed to insert an IRES-Cre bicistronic expression cassette and frt-flanked neo cassette into the 3' UTR of exon 4 of the targeted locus. The construct was electroporated into 129S1/Sv-derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric males were bred with C57BL/6J females to establish the colony. Mice heterozygous for this Osr2-IresCre (or Osr2^IresCre) allele were bred together to generate homozygotes prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Osr2^tm2(cre)Jian
Name: targeted mutation 2, Rulang Jiang
MGI accession ID: MGI:3768549
Generation method: Targeted
Attributes: Recombinase-expressing
Marker symbol: Osr2
Marker name: odd-skipped related 2
Chromosome: 15
Strain of origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Expression of cre is detected in the palate and kidney during development.
Molecular note: An IRES-cre cassette and an frt-flanked neo were inserted downstream of the stop codon. Expression of cre is detected in the palate and kidney during development.