The Mark3 (MAP/microtubule affinity-regulating kinase) targeted in this strain is associated with the Cdc25c cell division cycle 25 homolog C) gene. Western blot analysis confirms that expression of this targeted gene is eliminated in brain, spleen, thymus, white and brown adipose tissue, large and small intestines, stomach, kidney and liver. A phenotype has not as yet been detected.
Dr. Helen Piwnica-Worms, MD Anderson Cancer Center
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Mark3 | MAP/microtubule affinity regulating kinase 3 |
The protein kinase targeted in this strain is associated with the Cdc25c cell division cycle 25 homolog C) gene. Western blot analysis confirms that expression of this targeted gene is eliminated in brain, spleen, thymus, white and brown adipose tissue, large and small intestines, stomach, kidney and liver. A phenotype has not as yet been detected.
A 1.6 kb fragment of the gene was disrupted by the insertion of a neomycin resistance cassette. Exon 2 (hosting the ATP binding site) and the 5' slice site of intron 2/3 were shifted out of frame. The mutation was created using 129X1/SvJ-derived RW-4 embryonic stem (ES) cells. This line was backcrossed nine times to C57BL/6 by the donating investigator.
Allele Name | targeted mutation 1, Helen Piwnica-Worms |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | cTak KO; Par-1a- |
Gene Symbol and Name | Mark3, MAP/microtubule affinity regulating kinase 3 |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | 12 |
Molecular Note | A 1.9 kb fragment of the gene was disrupted by the insertion of a neomycin resistance cassette. Exon 2 (hosting the ATP binding site) and the 5' slice site of intron 2/3 were shifted out of frame. Multi-tissue Western blotting confirmed ablation of this gene in all tissues of homozygous null-mice. |
Mutations Made By | Dr. Helen Piwnica-Worms, MD Anderson Cancer Center |
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the B6.129X1-Mark3tm1Hpw/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #009366 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-Type for Mark3<tm1Hpw> |
Frozen Mouse Embryo | B6.129X1-Mark3<tm1Hpw>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129X1-Mark3<tm1Hpw>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129X1-Mark3<tm1Hpw>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129X1-Mark3<tm1Hpw>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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