B6.129X1-Mark3^tm1Hpw/J

Stock number: 009366

Description

The Mark3 (MAP/microtubule affinity-regulating kinase) targeted in this strain is associated with the Cdc25c (cell division cycle 25 homolog C) gene. Western blot analysis confirms that expression of this targeted gene is eliminated in brain, spleen, thymus, white and brown adipose tissue, large and small intestines, stomach, kidney and liver. A phenotype has not as yet been detected.

Detailed description

The protein kinase targeted in this strain is associated with the Cdc25c (cell division cycle 25 homolog C) gene. Western blot analysis confirms that expression of this targeted gene is eliminated in brain, spleen, thymus, white and brown adipose tissue, large and small intestines, stomach, kidney and liver. A phenotype has not as yet been detected.

Development

A 1.6 kb fragment of the gene was disrupted by the insertion of a neomycin resistance cassette. Exon 2 (hosting the ATP binding site) and the 5' slice site of intron 2/3 were shifted out of frame. The mutation was created using 129X1/SvJ-derived RW-4 embryonic stem (ES) cells. This line was backcrossed nine times to C57BL/6J by the donating investigator.

Upon arrival at The Jackson Laboratory, sperm was cryopreserved. Cryorecovery/live colony development will involve C57BL/6J (Stock No. 000664).

Allele

Symbol: Mark3^tm1Hpw
Name: targeted mutation 1, Helen Piwnica-Worms
MGI accession ID: MGI:3836595
Generation method: Targeted
Attributes: Null/Knockout
Synonyms: cTak KO; Par-1a^-
Marker symbol: Mark3
Marker name: MAP/microtubule affinity regulating kinase 3
Marker synonyms: 1600015G02Rik; A430080F22Rik; CTAK1; C-TAK1; ETK-1; KP78; mKIAA1860; PAR1A; Par-1a; VIPB
Chromosome: 12
Strain of origin: 129X1/SvJ
Molecular note: A 1.9 kb fragment of the gene was disrupted by the insertion of a neomycin resistance cassette. Exon 2 (hosting the ATP binding site) and the 5' slice site of intron 2/3 were shifted out of frame. Multi-tissue Western blotting confirmed ablation of this gene in all tissues of homozygous null-mice.