Mice hemizygous for the CDX2P9.5-G22Cre transgene are viable and fertile. The 9.5 kb human caudal type homeo box 2 (CDX2) promoter/enhancer sequence is prevented from driving nuclear-localized Cre recombinase expression as a 22 guanine nucleotide repeat tract between initiating methionine (ATG) codon and the remainder of the coding region alters the cre reading frame. Presumably, following a somatic mutation that results in some frameshift mutation(s) in the guanine nucleotide tract in a subset of somatic cells, resultant expression of a functional Cre recombinase is observed predominantly in colonic epithelium during late gestation and in adult tissues. Specifically, mosaic Cre recombinase expression would be observed in epithelium from the distal ileum and cecum, and mainly the proximal and distal colon from the crypt base to the luminal surface. Cre recombinase expression may also be observed in a few caudal-derived cells during early development. When these transgenic mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination is expected to result in deletion of the floxed sequences in these tissues of the offspring that reactivate cre expression. These CDX2P9.5-G22Cre transgenic mice may be useful as a somatic mutation reporter strain and/or to generate conditional mutations in colorectal epithelial cells.

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These CDX2P9.5-G22Cre transgenic mice harbor a nuclear-localized Cre recombinase with 22 guanine nucleotide repeat between initiating methionine (ATG) codon and the remainder of the coding region altering the cre reading frame. Presumably, following a somatic mutation that results in some frameshift mutation(s) in the guanine nucleotide tract in a subset of somatic cells, resultant expression of a functional Cre recombinase is driven by a 9.5 kb human caudal type homeo box 2 (CDX2) promoter/enhancer sequence. These CDX2P9.5-G22Cre transgenic mice may be useful as a somatic mutation reporter strain and/or to generate conditional mutations in colorectal epithelial cells.

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