This strain carries an inverted human placental alkaline phosphatase (ALPP; PLAP) coding segment flanked by oppositely-oriented loxP sites integrated in the Gt(ROSA)26Sor gene. Cre excision of the floxed segment flips rather than excises the ALPP inversion and reinstates a transcriptionally active form of the gene. This reporter can be activated by tissue-specific cre recombinase in any cell in the body at any time in development. The result is very clear ALPP enzyme activity that can be detected histochemically.
Jeremy Nathans, Johns Hopkins University
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
This strain carries an inverted human placental alkaline phosphatase (ALPP; PLAP) coding segment flanked by oppositely-oriented loxP sites integrated in the Gt(ROSA)26Sor gene. Cre excision of the floxed segment flips rather than excises the ALPP inversion and reinstates a transcriptionally active form of the gene. This reporter can be activated by tissue-specific cre recombinase in any cell in the body at any time in development. The result is very clear ALPP enzyme activity that can be detected histochemically. Heterozygotes and homozygotes are normal in size, viability, and fertility.
The human placental alkaline phosphatase (ALPP; PLAP) coding region was engineered to contain a single intron with a loxP site. Another loxP site in inverted orientation was placed 3' of the ALPP coding region. A single round of Cre-mediated recombination in E. coli resulted in the inversion of the 3' half of the coding region, thereby disrupting the ALPP open reading frame. A PGK-neo site is located downstream of the 3' UTR. This construct, referred to as "AP inversion" or "AP inv", was knocked into the ubiquitously-expressed Gt(ROSA)26Sor locus by homologous recombination. A 129-derived embryonic stem cell line was used to create the mutation. This line was backcrossed three times to C57BL/6 by the donating laboratory.
Expressed Gene | ALPP, alkaline phosphatase, placental, human |
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Site of Expression |
Allele Name | targeted mutation 2, Jeremy Nathans |
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Allele Type | Targeted (Reporter) |
Allele Synonym(s) | R26AP inv; R26IAP; ROSA26-AP inversion; Rosa26-iAP |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | ALPP, alkaline phosphatase, placental, human |
Strain of Origin | 129 |
Chromosome | 6 |
Molecular Note | The human placental alkaline phosphatase (ALPP; PLAP) coding region was engineered to contain a single intron with a loxP site. Another loxP site in inverted orientation was placed 3' of the ALPP coding region. A single round of Cre-mediated recombination in E. coli resulted in the inversion of the 3' half of the coding region, thereby disrupting the ALPP open reading frame. This construct, referred to as "AP inversion" or "AP inv", was knocked into the ubiquitously-expressed Gt(ROSA)26Sor locus by homologous recombination. |
Mutations Made By | Jeremy Nathans, Johns Hopkins University |
When maintained as a live colony, homozygotes or heterozygotes may be bred together.
When using the ROSA26-AP Inversion (R26iAP) mouse strain in a publication, please cite the originating article(s) and include JAX stock #009253 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Gt(ROSA)26Sor<tm2Nat> |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm2Nat>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm2Nat>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm2Nat>/J | $3373.50 |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm2Nat>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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