Mice that are homozygous for this targeted mutation of Tia1 (cytotoxic granule-associated RNA binding protein 1) develop mild arthritis and are more susceptible to endotoxin shock. This mutant mouse strain may be useful in studies of stress-induced translational arrest and silencing, inflammation, and arthritis.
Paul Anderson, Harvard Medical School
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Tia1 | cytotoxic granule-associated RNA binding protein 1 |
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (protein) is detected by immunoblot analysis of embryonic fibroblast lysates. Homozygotes are more susceptible to LPS-induced endotoxin shock than wildtype controls and develop mild arthritis. Macrophages derived from these animals produce significantly more TNF-alpha than wild-type controls. Mutant mice exhibit altered polysome disassembly. This mutant mouse strain may be useful in studies of stress-induced translational arrest and silencing, inflammation, and arthritis.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt parts of intron 3 and exon 4. The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into BALB/c blastocysts. The resulting chimeric animals were crossed to BALB/c mice, and then backcrossed to BALB/c for 3 generations. The mice were then backcrossed to C57BL/6 for 14 generations before arriving at The Jackson Laboratory.
Allele Name | targeted mutation 1, Paul Anderson |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | TIA-1- |
Gene Symbol and Name | Tia1, cytotoxic granule-associated RNA binding protein 1 |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 6 |
Molecular Note | The gene was disrupted by replacement of parts of intron 3 and exon 4 of the genomic DNA with a neomycin cassette via homologous recombination. Southern blot analysis confirmed recombination and immunoprecipitation demonstrated that no detectable protein is produced from this allele. |
Mutations Made By | Paul Anderson, Harvard Medical School |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6.129S2(C)-Tia1tm1Andp/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #009248 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Tia1<tm1Andp> |
Frozen Mouse Embryo | B6.129S2(C)-Tia1<tm1Andp>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S2(C)-Tia1<tm1Andp>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S2(C)-Tia1<tm1Andp>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S2(C)-Tia1<tm1Andp>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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