B6.129S2(C)-Tia1^tm1Andp/J

Stock number: 009248
Research areas: Cell Biology Research, Immunology, Inflammation and Autoimmunity Research

Description

Mice that are homozygous for this targeted mutation of Tia1 (cytotoxic granule-associated RNA binding protein 1) develop mild arthritis and are more susceptible to endotoxin shock. This mutant mouse strain may be useful in studies of stress-induced translational arrest and silencing, inflammation, and arthritis.

Detailed description

Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (protein) is detected by immunoblot analysis of embryonic fibroblast lysates. Homozygotes are more susceptible to LPS-induced endotoxin shock than wildtype controls and develop mild arthritis. Macrophages derived from these animals produce significantly more TNF-alpha than wild-type controls. Mutant mice exhibit altered polysome disassembly. This mutant mouse strain may be useful in studies of stress-induced translational arrest and silencing, inflammation, and arthritis.

Development

A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt parts of intron 3 and exon 4. The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into BALB/c blastocysts. The resulting chimeric animals were crossed to BALB/c mice, and then backcrossed to BALB/c for 3 generations. The mice were then backcrossed to C57BL/6 for 14 generations before arriving at The Jackson Laboratory.

Allele

Symbol: Tia1^tm1Andp
Name: targeted mutation 1, Paul Anderson
MGI accession ID: MGI:3037091
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Tia1
Marker name: cytotoxic granule-associated RNA binding protein 1
Chromosome: 6
Strain of origin: 129S2/SvPas
Molecular note: The gene was disrupted by replacement of parts of intron 3 and exon 4 of the genomic DNA with a neomycin cassette via homologous recombination. Southern blot analysis confirmed recombination and immunoprecipitation demonstrated that no detectable protein is produced from this allele.