Overview

Also Known As: Conductin^lacZ

The Axin2^lacZ mutation both abolishes endogenous gene function and expresses NLS-lacZ under the control of the endogenous promoter/enhancer regions. Homozygous mice exhibit a phenotype resembling craniosynostosis in humans.

Donating Investigator

Walter Birchmeier, Max-Delbrueck-Center for Mol. Medicine

Genetic overview

Genetic Background	Generation
	N16+N1F9 (2019-03-19 00:00:00)

Axin2^tm1Wbm

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter, Null/Knockout)	Axin2	axin 2

View Genetics

Research Applications

Developmental Biology Research
Sensorineural Research
Cell Biology Research
Mouse/Human Gene Homologs
Research Tools
Neurobiology Research

View All Research Applications

Base Price

Starting at:

$278.00 Domestic price for female

356.51 Domestic price for breeder pair

View Price List

Details

Detailed Description

Homozygous mice are viable and fertile, with the Axin2^lacZ (or conductin^lacZ) mutation that both abolishes endogenous Axin2 gene function and expresses NLS-lacZ under the control of the endogenous Axin2 promoter/enhancer regions. Homozygous mice exhibit cranial skull defects and malformations of skull structures; a phenotype resembling craniosynostosis in humans. Specifically, homozygous mice show an obvious reduction in head growth within the first 3 weeks after birth, resulting from developmental defects of the cranial skull (premature fusion of cranial sutures) at early postnatal stages. Axin2-deficient mice have abnormal calvarial morphogenesis/osteoblast development. Because Axin2 is a negative regulator of the canonical Wnt pathway that suppress signal transduction by promoting β-catenin degradation, the NLS-lacZ expression in these Axin2^lacZ (or conductin^lacZ) mutant mice may be useful in monitoring endogenous canonical Wnt signals in many tissues and organs during development, regeneration and tumorigenesis.

C57BL/6N-derived mice are homozygous for the recessive mutation retinal degeneration 8 (Crb1^rd8) - identified as a single base deletion in the Crb1 gene that causes a frame shift and premature stop codon that truncates the transmembrane and cytoplasmic domain of the protein.
Mice homozygous for Crb1^rd8 exhibit retinal external limiting membrane fragmentation and outer retinal dysplasia, as well as multifocal retinal degeneration and the accumulation of subretinal microglia/macrophages [Mattapallil et al. 2012 Invest Ophthalmol Vis Sci. 53:2921 , Mehalow et al. 2003 Hum Mol Genet. 12:2179 ,
Luhmann et al. 2012 PLoS One 7:e35551]. Of note, C57BL/6J-derived mice are homozygous for the wildtype Crb1 allele.

Development

A targeting vector was used delete most of exon 2 of the Axin2 (Axin2/conductin/Axil) locus via in-frame insertion of a nuclear-localized β-galactosidase (NLS-lacZ) gene (followed by a polyA signal and neo cassette) into the endogenous Axin2 start codon. This construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. ES cells were injected into recipient blastocysts, and chimeric mice were bred with C57BL/6NCrl mice to establish the mutant colony. The donating investigator reports that mutant mice were subsequently backcrossed to C57BL/6NCrl inbred mice for 15 generations prior to sending to The Jackson Laboratory Repository in 2009. Upon arrival, sperm was cryopreserved.
To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6NJ oocytes (Stock No. 005304).

Of note, it is not known if the Y chromosome has been fixed to the C57BL/6N background during backcrossing.

Expression Data

Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression	LacZ with a nuclear localization signal (NLS-lacZ) replaces expression of the targeted gene and may be useful in monitoring endogenous canonical Wnt signals in many tissues during development, regeneration and tumorigenesis.

Control Suggestions

Wild-type from the colony
005304 C57BL/6NJ

Additional Information

Considerations for Choosing Controls

Selected References

Lustig B; Jerchow B; Sachs M; Weiler S; Pietsch T; Karsten U; van de Wetering M; Clevers H; Schlag PM; Birchmeier W; Behrens J. 2002. Negative feedback loop of Wnt signaling through upregulation of conductin/axin2 in colorectal and liver tumors. Mol Cell Biol 22(4):1184-93PubMed: 11809809 MGI: J:74286

View All References

Genetics

Axin2^tm1Wbm

Allele Symbol: Axin2^tm1Wbm

Allele Name	targeted mutation 1, Walter Birchmeier
Allele Type	Targeted (Reporter, Null/Knockout)
Allele Synonym(s)	Axin2^tm1Wbm; targeted mutation 1, Walter Birchmeier
Gene Symbol and Name	Axin2, axin 2
Gene Synonym(s)	AXIL; ODCRCS; Conductin; axil; Axil; axin-2
Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression	LacZ with a nuclear localization signal (NLS-lacZ) replaces expression of the targeted gene and may be useful in monitoring endogenous canonical Wnt signals in many tissues during development, regeneration and tumorigenesis.
Strain of Origin	129P2/OlaHsd
Chromosome	11
Molecular Note	A lacZ gene containing a nuclear localization signal was introduced in frame to the endogenous start codon by homologous recombination, thereby replacing most of exon 2. Beta-galactosidase staining reflected endogenous gene expression in the developing embryo.
Mutations Made By	Walter Birchmeier, Max-Delbrueck-Center for Mol. Medicine

Disease/Phenotype

Disease Terms

Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).

craniosynostosis

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Crb1^rd8 related

Developmental Biology Research
- Eye Defects
Sensorineural Research
- Retinal Degeneration
Cell Biology Research
- Defects in Cell Adhesion Molecules
Mouse/Human Gene Homologs
- Retinitis pigmentosa 12; Leber congenital amaurosis (CLA)

Developmental Biology Research
- Growth Defects
  - Growth Defects (homozygous)
- Internal/Organ Defects
  - brain
- Craniofacial and Palate Defects
Research Tools
- lacZ Expression
- Neurobiology Research
  - cell marker
- Genetics Research
  - Tissue/Cell Markers
Neurobiology Research
- lacZ expression in neural tissue
Cell Biology Research
- Signal Transduction

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Axin2^tm1Wbm/Axin2^tm1Wbm
involves: 129P2/OlaHsd * C57BL/6

cellular phenotype

abnormal osteoblast physiology
- primary calvarial osteoblasts isolated from nasal/frontal bones exhibited enhanced formation of mineralized nodules when induced to differentiate

enhanced osteoblast differentiation
- enhanced osteoblast differentiation predominantly in the anterior calvarium

increased osteoblast proliferation
- enhanced proliferation of osteoblast precursors in the developing metopic suture and of isolated primary osteoblasts from nasal/frontal bones (cranial neural crest derived) but not from parietal bones (mesoderm-derived)

skeleton phenotype

abnormal cranium morphology
- adult skulls became abnormally structured and shaped due to premature suture closure

abnormal neurocranium morphology
- enhanced osteoblast differentiation predominantly in the anterior calvarium

abnormal osteoblast physiology
- primary calvarial osteoblasts isolated from nasal/frontal bones exhibited enhanced formation of mineralized nodules when induced to differentiate

abnormal skeleton development
- accelerated skeletogenesis

absent jugum limitans
- the jugum limitans is often missing

decreased cranium height

enhanced osteoblast differentiation
- enhanced osteoblast differentiation predominantly in the anterior calvarium

increased osteoblast proliferation
- enhanced proliferation of osteoblast precursors in the developing metopic suture and of isolated primary osteoblasts from nasal/frontal bones (cranial neural crest derived) but not from parietal bones (mesoderm-derived)

premature intramembranous bone ossification
- in the skull

premature metopic suture closure
- premature closure of the metopic suture, with the suture remaining patent at birth but fused by P8

vision/eye phenotype

abnormal eye morphology
- requently exhibited eye abnormalities

craniofacial phenotype

abnormal cranium morphology
- adult skulls became abnormally structured and shaped due to premature suture closure

abnormal neurocranium morphology
- enhanced osteoblast differentiation predominantly in the anterior calvarium

absent jugum limitans
- the jugum limitans is often missing

decreased cranium height

shortened head
- reduced growth of the head, obvious within 3 weeks after birth

growth/size/body region phenotype

shortened head
- reduced growth of the head, obvious within 3 weeks after birth

References

Lustig B; Jerchow B; Sachs M; Weiler S; Pietsch T; Karsten U; van de Wetering M; Clevers H; Schlag PM; Birchmeier W; Behrens J. 2002. Negative feedback loop of Wnt signaling through upregulation of conductin/axin2 in colorectal and liver tumors. Mol Cell Biol 22(4):1184-93PubMed: 11809809 MGI: J:74286

Additional - Axin2^tm1Wbm related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR: Axin2^tm1Wbm
- Probe: Axin2^tm1Wbm Probe
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to C57BL/6NJ inbred mice (Stock No. 005304).
- Additional Breeding and Husbandry Support
Mating System
- Heterozygote x +/+ sibling
Citation
When using the Conductin^lacZ mouse strain in a publication, please cite the originating article(s) and include JAX stock #009120 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX10 (Standard)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Breeder Pair

Sex

Genotype

Price

Female
Male

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or wildytpe for Axin2<tm1Wbm>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo

$2,595.00 per straw or vial

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

Credit for PRODUCTS or SERVICES

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.

Animal Care and Use for SERVICES

Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Also Known As: ConductinlacZ

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Axin2tm1Wbm

Allele Symbol: Axin2tm1Wbm

Disease Terms

Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Crb1rd8 related

Mammalian Phenotype Terms by Genotype

Genotype: Axin2tm1Wbm/Axin2tm1Wbminvolves: 129P2/OlaHsd * C57BL/6

cellular phenotype

skeleton phenotype

vision/eye phenotype

craniofacial phenotype

growth/size/body region phenotype

References

Additional - Axin2tm1Wbm related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Breeder Pair

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Also Known As: Conductin^lacZ

Axin2^tm1Wbm

Allele Symbol: Axin2^tm1Wbm

Genotype: Crb1^rd8 related

Genotype: Axin2^tm1Wbm/Axin2^tm1Wbm
involves: 129P2/OlaHsd * C57BL/6

Additional - Axin2^tm1Wbm related