B6.129P2(Cg)-Hprt^{tm13(Ple54-EGFP)Ems}/Mmjax

Stock number: 009113
Strain synonyms: B6.129P2(Cg)-Hprt^{tm13(Ple54-EGFP)Ems}
Research areas: Neurobiology Research, Research Tools

Description

These mice have the Ple54-EGFP transgene targeted as a single copy "knockin" into the upstream region of the Hprt locus on the X chromosome. The promoter/regulatory regions of the human DCX gene directs expression of EGFP.

Allele

Symbol: Hprt^{tm13(Ple54-EGFP)Ems}
Name: targeted mutation 13, Elizabeth M Simpson
MGI accession ID: MGI:3835524
Generation method: Targeted
Attributes: Reporter
Marker symbol: Hprt
Marker name: hypoxanthine guanine phosphoribosyl transferase
Chromosome: X
Strain of origin: (B6.129P2-Hprt^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: Detection of EGFP is strongest in soma of cells in the neurogenic regions of the adult brain: the subgranular zone of the dentate gyrus as well as the forebrain subventricular zone (rostral migratory stream and olfactory bulb). Additionally, EGFP-positive cell bodies are detected in the neuropil of the cortex, hippocampus and brainstem. Double label immunocytochemistry shows that the EGFP -positive cells of the forebrain subventricular zone and the rostral migratory stream co-express Doublecortin.
Molecular note: The Ple54-EGFP/NLS transgene (pEMS1200) was designed with the 3687 bp Ple54 minipromoter (DCX-C; derived from a portion of the promoter and two downstream putative regulatory elements from the human doublecortin (DCX) gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome.

Detection of EGFP is strongest in soma of cells in the neurogenic regions of the adult brain: the subgranular zone of the dentate gyrus as well as the forebrain subventricular zone (rostral migratory stream and olfactory bulb). Additionally, EGFP-positive cell bodies are detected in the neuropil of the cortex, hippocampus and brainstem. Double label immunocytochemistry shows that the EGFP-positive cells of the forebrain subventricular zone and the rostral migratory stream co-express Doublecortin.
General note: Germ line transmission of mutant cell line mEMS1927 has been established.