| Allele Name | targeted mutation 13, Elizabeth M Simpson |
| Allele Type | Targeted (Reporter) |
| Allele Synonym(s) | DCX-EGFP; Hprt1tm13(mEMS1927)Ems; Hprt1tm13(Ple54-EGFP;mEMS1927)Ems; Ple54 |
| Gene Symbol and Name | Hprt, hypoxanthine guanine phosphoribosyl transferase |
| Gene Synonym(s) | |
| Promoter | DCX, doublecortin, human |
|---|
| Expressed Gene | GFP, Green Fluorescent Protein, |
|---|
| Site of Expression | Detection of EGFP is strongest in soma of cells in the neurogenic regions of the adult brain: the subgranular zone of the dentate gyrus as well as the forebrain subventricular zone (rostral migratory stream and olfactory bulb). Additionally, EGFP-positive cell bodies are detected in the neuropil of the cortex, hippocampus and brainstem. Double label immunocytochemistry shows that the EGFP -positive cells of the forebrain subventricular zone and the rostral migratory stream co-express Doublecortin. |
|---|
| Strain of Origin | (B6.129P2-Hprtb-m3/J x 129S-Gt(ROSA)26Sortm1Sor/J)F1 |
| Chromosome | X |
| General Note | Germ line transmission of mutant cell line mEMS1927 has been established. |
| Molecular Note | The Ple54-EGFP/NLS transgene (pEMS1200) was designed with the 3687 bp Ple54 minipromoter (DCX-C; derived from a portion of the promoter and two downstream putative regulatory elements from the human doublecortin (DCX) gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. Detection of EGFP is strongest in soma of cells in the neurogenic regions of the adult brain: the subgranular zone of the dentate gyrus as well as the forebrain subventricular zone (rostral migratory stream and olfactory bulb). Additionally, EGFP-positive cell bodies are detected in the neuropil of the cortex, hippocampus and brainstem. Double label immunocytochemistry shows that the EGFP-positive cells of the forebrain subventricular zone and the rostral migratory stream co-express Doublecortin. |
| Mutations Made By | Elizabeth Simpson, Centre for Molecular Medicine & Therapeutics, University of British Columbia |
