These Myd88fl mice have loxP sites flanking exon 3 of the myeloid differentiation primary response gene 88 (Myd88). These mutant mice may be useful for Cre-lox studying signal transduction, Toll-like receptor signaling and natural killer cells.
IMR Colony, The Jackson Laboratory
These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissue(s).
When bred to a strain with inducible Cre recombinase expression in dendritic cells (see Stock No. 008068 for example), this mutant mouse strain may be useful in studies of Toll-like receptor signaling during immune responses.
When bred to a strain with Cre recombinase expression in hematopoietic cells (see Stock No. 008610 for example), this mutant mouse strain may be useful in studies of Toll-like receptor signaling and natural killer cells.
When bred to a strain with Cre recombinase expression in villi and crypt cells of the small and large intestines (see Stock No. 004586 for example), this mutant mouse strain may be useful in studies of microbial intestinal flora.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A FRT site flanked targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes (HSV-TK) and a diphtheria toxin A subunit cassette was utilized in the construction of this mutant. This selection cassette was inserted upstream of exon 3 of the targeted gene, and another loxP site was inserted downstream of exon 3. This construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to transgenic mice (on a congenic C57BL/6 genetic background) expressing FLPe recombinase under the control of the actin beta (ACTB) promoter to remove the selection cassette. Mice that retained the loxP site flanked exon 3 (and residual single FRT site) were then backcrossed to C57BL/6J for 11 generations before arriving at The Jackson Laboratory (as Stock No. 008888). Upon arrival, some mice were backcrossed to BALB/cByJ inbred mice (Stock No. 001026) for at least 5 generations to generate this congenic strain (Stock No. 009108).
|Allele Name||targeted mutation 1, Anthony L DeFranco|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Myd88, myeloid differentiation primary response gene 88|
|Strain of Origin||129P2/OlaHsd|
|Mutations Made By|| |
Anthony DeFranco, UCSF
Mutant mice were bred to BALB/cByJ inbred mice (Stock No. 001026) for many generations to establish this congenic strain. When maintaining the live congenic colony, homozygous mice may be bred together.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders
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