CBy.129P2(B6)-Myd88^tm1Defr/J

Stock number: 009108
Product name: Myd88^fl
Research areas: Cancer Research, Cell Biology Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These Myd88^fl mice have loxP sites flanking exon 3 of the myeloid differentiation primary response gene 88 (Myd88). These mutant mice may be useful for Cre-lox studying signal transduction, Toll-like receptor signaling and natural killer cells.

Detailed description

These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissue(s).

When bred to a strain with inducible Cre recombinase expression in dendritic cells (see Stock No. 008068 for example), this mutant mouse strain may be useful in studies of Toll-like receptor signaling during immune responses.

When bred to a strain with Cre recombinase expression in hematopoietic cells (see Stock No. 008610 for example), this mutant mouse strain may be useful in studies of Toll-like receptor signaling and natural killer cells.

When bred to a strain with Cre recombinase expression in villi and crypt cells of the small and large intestines (see Stock No. 004586 for example), this mutant mouse strain may be useful in studies of microbial intestinal flora.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

A FRT site flanked targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes (HSV-TK) and a diphtheria toxin A subunit cassette was utilized in the construction of this mutant. This selection cassette was inserted upstream of exon 3 of the targeted gene, and another loxP site was inserted downstream of exon 3. This construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to transgenic mice (on a congenic C57BL/6 genetic background) expressing FLPe recombinase under the control of the actin beta (ACTB) promoter to remove the selection cassette. Mice that retained the loxP site flanked exon 3 (and residual single FRT site) were then backcrossed to C57BL/6J for 11 generations before arriving at The Jackson Laboratory (as Stock No. 008888). Upon arrival, some mice were backcrossed to BALB/cByJ inbred mice (Stock No. 001026) for at least 5 generations to generate this congenic strain (Stock No. 009108).

Allele

Symbol: Myd88^tm1Defr
Name: targeted mutation 1, Anthony L DeFranco
MGI accession ID: MGI:3809600
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Myd88
Marker name: myeloid differentiation primary response gene 88
Chromosome: 9
Strain of origin: 129P2/OlaHsd
Molecular note: Exon 3 was flanked by loxP sites by placing a FRT-flanked neo-cassette with a 3' loxP site upstream of exon 3 with an additional loxP site being placed downstream of exon 3. Founders were crossed with ACTB-FLPe mice to remove the neo selection cassette. Expression levels of Myd88 were unaffected by loxP insertion. Cre-recombination is predicted to excise exon 3 and create a null allele.