Mice homozygous for this floxed Gcnt1 (glucosaminyl (N-acetyl) transferase 1, core 2) targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exon is deleted by cre expression to produce a null allele.
Jamey D Marth, Burnham Inst at Univ Calif Santa Barbara
|Allele Name||targeted mutation 2, Jamey Marth|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||C2 GlcNAcT |
|Gene Symbol and Name||Gcnt1, glucosaminyl (N-acetyl) transferase 1, core 2|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||The single protein coding exon was flanked upstream by a loxP site and downstream by a floxed neomycin resistance cassette. Transient Cre expression in correctly targeted cells resulted in removal of only the neomycin cassette in some ES cells.|
|Mutations Made By|| |
Jamey Marth, Burnham Inst at Univ Calif Santa Barbara
When maintained as a live colony, heterozygotes or homozygotes may be bred.
When using the B6;129-Gcnt1tm2Jxm/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #009076 in your Materials and Methods section.