These mitochondrial cyclophilin knockout mice may be useful in studying mitochondrial-driven cell death, as well as brain and cardiac injury.
Jeffery D. Molkentin, Cincinnati Children's Hospital
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Ppif | peptidylprolyl isomerase F (cyclophilin F) |
Mitochondria isolated from the livers, hearts, and brains of homozygous targeted mutant mice are resistant to swelling and permeability transition in vitro. Hepatocytes and fibroblasts are largely protected from Ca2+-overload and oxidative stress-induced cell death. Mice are protected from ischaemia/reperfusion-induced cell death in vivo. This strain may be useful in studies of mitochondrial-driven cell death.
The first three coding exons were replaced with a neomycin resistance cassette. A 129-dervived emabryonic stem (ES) cell line was used to create the mutation. Chimeras were crossed to C57BL/6 and the strain was maintained on a mixed C57BL/6 and 129 genetic background by the donating laboratory.
Allele Name | targeted mutation 1, Jeffery D Molkentin |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Ppif- |
Gene Symbol and Name | Ppif, peptidylprolyl isomerase F (cyclophilin F) |
Gene Synonym(s) | |
Strain of Origin | 129 |
Chromosome | 14 |
Molecular Note | A neomycin resistance gene replaced the first three coding exons. Western blot of cardiac protein extracts from mutants demonstrated the lack of protein. |
Mutations Made By | Jeffery Molkentin, Cincinnati Children's Hospital |
When maintained as a live colony, homozygotes or heterozygotes may be bred. Homozygotes are smaller than wildtype mice and are prone to obesity as they age.
When using the Ppif- mouse strain in a publication, please cite the originating article(s) and include JAX stock #009071 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Ppif<tm1Jmol> |
Frozen Mouse Embryo | B6;129-Ppif<tm1Jmol>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Ppif<tm1Jmol>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Ppif<tm1Jmol>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129-Ppif<tm1Jmol>/J Frozen Embryo | $3373.50 |
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