B6;129-Ppif^tm1Jmol/J

Stock number: 009071
Product name: Ppif^-
Research areas: Apoptosis Research, Cell Biology Research

Description

These mitochondrial cyclophilin knockout mice may be useful in studying mitochondrial-driven cell death, as well as brain and cardiac injury.

Detailed description

Mitochondria isolated from the livers, hearts, and brains of homozygous targeted mutant mice are resistant to swelling and permeability transition in vitro. Hepatocytes and fibroblasts are largely protected from Ca²⁺-overload and oxidative stress-induced cell death. Mice are protected from ischaemia/reperfusion-induced cell death in vivo. This strain may be useful in studies of mitochondrial-driven cell death.

Development

The first three coding exons were replaced with a neomycin resistance cassette. A 129-dervived emabryonic stem (ES) cell line was used to create the mutation. Chimeras were crossed to C57BL/6 and the strain was maintained on a mixed C57BL/6 and 129 genetic background by the donating laboratory.

Allele

Symbol: Ppif^tm1Jmol
Name: targeted mutation 1, Jeffery D Molkentin
MGI accession ID: MGI:3577093
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Ppif
Marker name: peptidylprolyl isomerase F (cyclophilin F)
Chromosome: 14
Strain of origin: 129
Molecular note: A neomycin resistance gene replaced the first three coding exons. Western blot of cardiac protein extracts from mutants demonstrated the lack of protein.