Homozygous Dusp6 (dual specificity phosphatase 6) knock-out mutant mice have enlarged hearts; cardiac hypercellularity due to greater rates of myocyte proliferation during embryonic development and in the early postnatal period. Embryonic fibroblasts derived from these mice show reduced apoptosis. This strain may be useful in studies of heart function and disease.
Jeffery D. Molkentin, Cincinnati Children's Hospital
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Dusp6 | dual specificity phosphatase 6 |
Homozygous targeted mutant mice have larger hearts than wild-type mice at every age examined. They show greater rates of myocyte proliferation during embryonic development and in the early postnatal period, resulting in cardiac hypercellularity. This lends protection against decompensation and hypertrophic cardiomyopathy following long term pressure overload and myocardial infarction injury in adult mice. Embryonic fibroblasts derived from these mice also show reduced apoptosis rates as compared to wildtype. Homozygotes are viable, fertile and otherwise overtly normal. This strain may be useful in studies of heart function and disease.
All three exons (exons 1-3) of the gene were replaced with a neomycin resistance gene. A 129-derived embryonic stem (ES) cell line was used to create the mutation. Chimeras were crossed with C57BL/6 and the line was maintained on a mixed C57BL/6 and 129 genetic background by the donating laboratory. At The Jackson Laboratory, the live colony was bred at least one generation to B6129SF2/J mice (Stock No. 101045).
Allele Name | targeted mutation 1, Jeffery D Molkentin |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Dusp6, dual specificity phosphatase 6 |
Gene Synonym(s) | |
Strain of Origin | 129 |
Chromosome | 10 |
Molecular Note | All 3 exons were replaced with a neo cassette via homologous recombination. Western blot analysis confirmed the absence of protein in the lung, spleen, thymus, and heart of homozygous mice. |
Mutations Made By | Jeffery Molkentin, Cincinnati Children's Hospital |
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the B6;129-Dusp6tm1Jmol/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #009069 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Dusp6<tm1Jmol> |
Frozen Mouse Embryo | B6;129-Dusp6<tm1Jmol>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Dusp6<tm1Jmol>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Dusp6<tm1Jmol>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129-Dusp6<tm1Jmol>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.