B6;129-Postn^tm1Jmol/J

Stock number: 009067
Research areas: Cardiovascular Research

Description

Homozyous Postn (periostin, osteoblast specific factor) targeted mutation mice are more prone to ventricular rupture in the first 10 days after a myocardial infarction, but surviving mice show less fibrosis and better ventricular performance. They also show less fibrosis and hypertrophy following long-term pressure overload. This strain may be useful in studies of cardiac remodeling and hypertrophy.

Detailed description

Homozyous targeted mutation mice are more prone to ventricular rupture in the first 10 days after a myocardial infarction, but surviving mice show less fibrosis and better ventricular performance. They also show less fibrosis and hypertrophy following long-term pressure overload. The developing feet and hearts of 1-day-old mice show no expression of protein. No significant protein is found in the extracellular matrix of the heart after postnatal development, although the valves show persistent expression within fibroblasts. Homozygotes are slightly smaller than wild-type and heterozygous animals. This strain may be useful in studies of cardiac remodeling and hypertrophy.

Development

Exons 4-10 were replaced with a neomycin resistance cassette placed in the reverse transcriptional orientation. A 129-derived embryonic stem (ES) cell line was used to create the mutation. Chimeric mice were crossed to C57BL/6. This line was maintained on a mixed C57BL/6 and 129 genetic background by the donating laboratory. Upon arrival at The Jackson Laboratory, this strain was bred to C57BL/6J inbred mice (Stock No. 000664) for at least one additional generation.

Allele

Symbol: Postn^tm1Jmol
Name: targeted mutation 1, Jeffery D Molkentin
MGI accession ID: MGI:3819350
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Postn
Marker name: periostin, osteoblast specific factor
Chromosome: 3
Strain of origin: 129/Sv
Molecular note: Exons 4 through 10 were replaced with a neo cassette. The absence of protein expression was confirmed by western blot analysis on P1 developing foot and heart extracts.