JAX Mouse Strain Datasheet - 009061

STOCK Osr1^{tm1(EGFP/cre/ERT2)Amc}/J

Stock No:: 009061

Targeted Mutation

Cryo Recovery

Overview

These mice harbor a Osr1^eGFPCreERt2 (Osr1^GCE) "knock-in" allele that both abolishes Osr1 (odd-skipped related 1 (Drosophila)) gene function and expresses EGFP and creERT2 from the Osr1 promoter/enhancer elements. While EGFP expression is observed in intermediate mesoderm and dorsal lateral plate mesoderm of developing embryos (~E8.5), Cre-ERT2 fusion gene activity is inducible; observed in the same cells only following tamoxifen administration. These Osr1^GCE mice may be useful for lineage-tracing/marking Osr1-expressing cells for studying metanephric kidney development, as well as vascular, heart, and urogenital development.

Donating Investigator

Andrew P McMahon, University of Southern California

Genetic overview

Genetic Background	Generation

Osr1^{tm1(EGFP/cre/ERT2)Amc}

Allele Type	Gene Symbol	Gene Name
Targeted (Inducible, Recombinase-expressing)	Osr1	odd-skipped related 1 (Drosophila)

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Research Applications

Cancer Research
Developmental Biology Research
Research Tools

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Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

Homozygous mice die upon birth with multiple abnormalities of the heart and urogenital system (among other tissues). Heterozygous mice are viable and fertile. The Osr1^eGFPCreERt2 (Osr1^GCE) "knock-in" allele both abolishes Osr1 gene function and expresses an eGFPCreERt2 fusion protein (EGFP and creERT2 fusion protein from the Osr1 promoter/enhancer elements). While EGFP immunofluorescence is observed in intermediate mesoderm and dorsal lateral plate mesoderm of developing embryos (~E8.5), Cre-ERT2 fusion gene activity is inducible; observed in the same cells only following tamoxifen administration. As such, when Osr1^GCE mice are bred with mice containing loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequences in the Osr1-expressing cells of the offspring. Of note, removal of the frt-flanked neo cassette is reported to result in ectopic Cre recombinase expression in the absence of tamoxifen.

The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered.

Development

A targeting vector was designed with a cDNA encoding an eGFPCreERt2 (GCE) fusion protein. Specifically, this targeting vector contained an enhanced green fluorescent protein (EGFP) and a CreERT2 fusion gene (Cre-ER^T2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), a polyA signal, and a frt-flanked PGK-neo-pA cassette. This construct was designed to replace the first ATG codon of the targeted gene with the first ATG codon of the GCE cassette. This construct was electroporated into F1 hybrid (129/Sv x C57BL/6J) embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with C57BL/6J mice. The donating investigator reports that mutant mice were backcrossed to C57BL/6J mice for several generations before arriving at The Jackson Laboratory (see SNP analysis note below). Upon arrival the mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

A 27 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed 10 of 27 markers (8 different chromosomes) that were not C57BL/6 allele-type. These data indicate the mice were not backcrossed to C57BL/6 prior to arrival at The Jackson Laboratory. No single source of genetic background contamination could be determined by this data. The donating investigator suggests there may be some Swiss Webster genetic contributions from a possible mating with R26Hoxd11 mutant mice many years ago.

Expression Data

Expressed Gene	GFP, Green Fluorescent Protein,
Expressed Gene	cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of Expression	GFP immunofluorescence is observed in intermediate mesoderm and dorsal lateral plate mesoderm of developing embryos (~E8.5).

Control Suggestions

Wild-type from the colony

Additional Information

Considerations for Choosing Controls

Selected References

Mugford JW; Sipila P; Kobayashi A; Behringer RR; McMahon AP. 2008. Hoxd11 specifies a program of metanephric kidney development within the intermediate mesoderm of the mouse embryo. Dev Biol 319(2):396-405. PubMed: 18485340 MGI: J:137524
Mugford JW; Sipila P; McMahon JA; McMahon AP. 2008. Osr1 expression demarcates a multi-potent population of intermediate mesoderm that undergoes progressive restriction to an Osr1-dependent nephron progenitor compartment within the mammalian kidney. Dev Biol 324(1):88-98. PubMed: 18835385 MGI: J:143306

View All References

Genetics

Osr1^{tm1(EGFP/cre/ERT2)Amc}

Allele Symbol: Osr1^{tm1(EGFP/cre/ERT2)Amc}

Allele Name	targeted mutation 1, Andrew P McMahon
Allele Type	Targeted (Inducible, Recombinase-expressing)
Allele Synonym(s)	Osr1^GCE; Osr1^eGFPCreERt2
Gene Symbol and Name	Osr1, odd-skipped related 1 (Drosophila)
Gene Synonym(s)	ODD; Odd1; Odd1
Expressed Gene	GFP, Green Fluorescent Protein,
Expressed Gene	cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of Expression	GFP immunofluorescence is observed in intermediate mesoderm and dorsal lateral plate mesoderm of developing embryos (~E8.5).
Strain of Origin	Not Specified
Chromosome	12
Molecular Note	The start codon was replaced with an eGFP/cre/ERT2 cassette followed by an frt flanked neo cassette via homologous recombination. GFP expression was detected in the intermediate mesoderm and dorsal lateral plate mesoderm at E8.5 and E9.5.
Mutations Made By	Andrew McMahon, University of Southern California

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Cancer Research
- Other
Developmental Biology Research
- Internal/Organ Defects
  - heart
  - heart: vasculature
  - kidney
  - urogenital
- Perinatal Lethality
  - Homozygous
Research Tools
- Cre-lox System
  - Cre Recombinase Expression
  - Cre Recombinase Expression: Inducible
- Developmental Biology Research
  - Cre-lox System
  - transplantation marker for embryonic and adult tissue
- Fluorescent Proteins
- Genetics Research
  - Mutagenesis and Transgenesis
  - Mutagenesis and Transgenesis: Cre-lox System
  - Tissue/Cell Markers
  - Tissue/Cell Markers: Cre-lox System
  - Tissue/Cell Markers: multiple
  - Tissue/Cell Markers: transplantation marker for embryonic and adult tissue

Genotype: GFP related

Research Tools
- Fluorescent Proteins

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Osr1^{tm1(EGFP/cre/ERT2)Amc}/Osr1⁺
Not Specified

normal phenotype

no abnormal phenotype detected
- viable and fertile

References

Mugford JW; Sipila P; Kobayashi A; Behringer RR; McMahon AP. 2008. Hoxd11 specifies a program of metanephric kidney development within the intermediate mesoderm of the mouse embryo. Dev Biol 319(2):396-405. PubMed: 18485340 MGI: J:137524
Mugford JW; Sipila P; McMahon JA; McMahon AP. 2008. Osr1 expression demarcates a multi-potent population of intermediate mesoderm that undergoes progressive restriction to an Osr1-dependent nephron progenitor compartment within the mammalian kidney. Dev Biol 324(1):88-98. PubMed: 18835385 MGI: J:143306

Additional - Osr1^{tm1(EGFP/cre/ERT2)Amc} related

Hu YC; Nicholls PK; Soh YQ; Daniele JR; Junker JP; van Oudenaarden A; Page DC. 2015. Licensing of primordial germ cells for gametogenesis depends on genital ridge signaling. PLoS Genet 11(3):e1005019. PubMed: 25739037 MGI: J:224163
Hu YC; Okumura LM; Page DC. 2013. Gata4 is required for formation of the genital ridge in mice. PLoS Genet 9(7):e1003629. PubMed: 23874227 MGI: J:199295
Liu H; Lan Y; Xu J; Chang CF; Brugmann SA; Jiang R. 2013. Odd-skipped related-1 controls neural crest chondrogenesis during tongue development. Proc Natl Acad Sci U S A 110(46):18555-60. PubMed: 24167250 MGI: J:202899
Mugford JW; Yu J; Kobayashi A; McMahon AP. 2009. High-resolution gene expression analysis of the developing mouse kidney defines novel cellular compartments within the nephron progenitor population. Dev Biol 333(2):312-23. PubMed: 19591821 MGI: J:152419
Xu J; Liu H; Park JS; Lan Y; Jiang R. 2014. Osr1 acts downstream of and interacts synergistically with Six2 to maintain nephron progenitor cells during kidney organogenesis. Development 141(7):1442-52. PubMed: 24598167 MGI: J:208647

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or wildytpe for Osr1<tm1(EGFP/cre/ERT2)Amc>

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Osr1tm1(EGFP/cre/ERT2)Amc

Allele Symbol: Osr1tm1(EGFP/cre/ERT2)Amc

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: GFP related

Mammalian Phenotype Terms by Genotype

Genotype: Osr1tm1(EGFP/cre/ERT2)Amc/Osr1+Not Specified

normal phenotype

References

Additional - Osr1tm1(EGFP/cre/ERT2)Amc related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Osr1^{tm1(EGFP/cre/ERT2)Amc}

Allele Symbol: Osr1^{tm1(EGFP/cre/ERT2)Amc}

Genotype: Osr1^{tm1(EGFP/cre/ERT2)Amc}/Osr1⁺
Not Specified

Additional - Osr1^{tm1(EGFP/cre/ERT2)Amc} related