C57BL/6-Apc^tm1Tyj/J

Stock number: 009045
Research areas: Cancer Research, Research Tools

Description

The 15 coding exons of the Apc (adenomatosis polyposis coli) gene are flanked by loxP sites. Germline heterozygous deletion of the floxed region results in a tumor-prone mouse similar to Apc^Min animals. This mutant mouse strain is useful for studies of this tumor suppressor gene and serves as a mouse model of colon cancer.

Detailed description

Mice that are homozygous for the conditional allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The 15 coding exons are flanked by loxP sites. Germline heterozygous deletion of the floxed region results in a tumor-prone mouse similar to Apc^Min animals (see Stock No. 002020). Homozygous germline deletion mice are not viable. This mutant mouse strain is useful for studies of this tumor suppressor gene and serves as a mouse model of colon cancer.

Development

A loxP-flanked puromycin resistance cassette was introduced to an intron 5' of the first coding exon. In vitro Cre-mediated recombination was used to excise the floxed region, leaving a single loxP site. Genomic regions 3' of the gene were then targeted with a loxP-FRT-neomycin-FRT cassette. In vivo germline FLPe-mediated recombination was done to excise the neomycin resistance gene leaving loxP and FRT sites 3' of the gene. This mutation was created using the C57BL/6-background v26.2 embryonic stem (ES) cell line and maintained on that background by the donating laboratory.

Allele

Symbol: Apc^tm1Tyj
Name: targeted mutation 1, Tyler Jacks
MGI accession ID: MGI:3829069
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Synonyms: APC^fle1-15
Marker symbol: Apc
Marker name: adenomatosis polyposis coli
Chromosome: 18
Strain of origin: C57BL/6
Molecular note: A loxP-flanked puromycin resistance cassette was introduced to an intron 5' of the first coding exon. Cre-mediated recombination was used to excise the floxed region, leaving a single loxP site. Genomic regions 3' of the gene were then targeted with a loxP-FRT-neomycin-FRT cassette. FLPe-mediated recombination was done to excise the neomycin resistance gene leaving loxP and FRT sites 3' of the gene.