The 15 coding exons of the Apc (adenomatosis polyposis coli) gene are flanked by loxP sites. Germline heterozygous deletion of the floxed region results in a tumor-prone mouse similar to ApcMin animals. This mutant mouse strain is useful for studies of this tumor suppressor gene and serves as a mouse model of colon cancer.
Dr. Tyler Jacks, Massachusetts Institute of Technology
Genetic Background | Generation |
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?+pN2F9
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Apc | adenomatosis polyposis coli |
Mice that are homozygous for the conditional allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The 15 coding exons are flanked by loxP sites. Germline heterozygous deletion of the floxed region results in a tumor-prone mouse similar to ApcMin animals (see Stock No. 002020). Homozygous germline deletion mice are not viable. This mutant mouse strain is useful for studies of this tumor suppressor gene and serves as a mouse model of colon cancer.
A loxP-flanked puromycin resistance cassette was introduced to an intron 5' of the first coding exon. In vitro Cre-mediated recombination was used to excise the floxed region, leaving a single loxP site. Genomic regions 3' of the gene were then targeted with a loxP-FRT-neomycin-FRT cassette. In vivo germline FLPe-mediated recombination was done to excise the neomycin resistance gene leaving loxP and FRT sites 3' of the gene. This mutation was created using the C57BL/6-background v26.2 embryonic stem (ES) cell line and maintained on that background by the donating laboratory.
Allele Name | targeted mutation 1, Tyler Jacks |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | APCfle1-15 |
Gene Symbol and Name | Apc, adenomatosis polyposis coli |
Gene Synonym(s) | |
Strain of Origin | C57BL/6 |
Chromosome | 18 |
Molecular Note | A loxP-flanked puromycin resistance cassette was introduced to an intron 5' of the first coding exon. Cre-mediated recombination was used to excise the floxed region, leaving a single loxP site. Genomic regions 3' of the gene were then targeted with a loxP-FRT-neomycin-FRT cassette. FLPe-mediated recombination was done to excise the neomycin resistance gene leaving loxP and FRT sites 3' of the gene. |
Mutations Made By | Dr. Tyler Jacks, Massachusetts Institute of Technology |
When maintained as a live colony, homozygotes may be bred.
When using the C57BL/6-Apctm1Tyj/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #009045 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Apc<tm1Tyj> |
Frozen Mouse Embryo | C57BL/6-Apc<tm1Tyj>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Apc<tm1Tyj>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Apc<tm1Tyj>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Apc<tm1Tyj>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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