The 15 coding exons of the Apc (adenomatosis polyposis coli) gene are flanked by loxP sites. Germline heterozygous deletion of the floxed region results in a tumor-prone mouse similar to ApcMin animals. This mutant mouse strain is useful for studies of this tumor suppressor gene and serves as a mouse model of colon cancer.
Dr. Tyler Jacks, Massachusetts Institute of Technology
Mice that are homozygous for the conditional allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The 15 coding exons are flanked by loxP sites. Germline heterozygous deletion of the floxed region results in a tumor-prone mouse similar to ApcMin animals (see Stock No. 002020). Homozygous germline deletion mice are not viable. This mutant mouse strain is useful for studies of this tumor suppressor gene and serves as a mouse model of colon cancer.
A loxP-flanked puromycin resistance cassette was introduced to an intron 5' of the first coding exon. In vitro Cre-mediated recombination was used to excise the floxed region, leaving a single loxP site. Genomic regions 3' of the gene were then targeted with a loxP-FRT-neomycin-FRT cassette. In vivo germline FLPe-mediated recombination was done to excise the neomycin resistance gene leaving loxP and FRT sites 3' of the gene. This mutation was created using the C57BL/6-background v26.2 embryonic stem (ES) cell line and maintained on that background by the donating laboratory.
|Allele Name||targeted mutation 1, Tyler Jacks|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Apc, adenomatosis polyposis coli|
|Gene Synonym(s)||AI047805; AU020952; AW124434; BTPS2; CC1; DP2; DP2.5; DP3; GS; Min; Min; PPP1R46; RATAPC; expressed sequence AI047805; expressed sequence AU020952; expressed sequence AW124434; multiple intestinal neoplasia|
|Strain of Origin||C57BL/6|
|Molecular Note||A loxP-flanked puromycin resistance cassette was introduced to an intron 5' of the first coding exon. Cre-mediated recombination was used to excise the floxed region, leaving a single loxP site. Genomic regions 3' of the gene were then targeted with a loxP-FRT-neomycin-FRT cassette. FLPe-mediated recombination was done to excise the neomycin resistance gene leaving loxP and FRT sites 3' of the gene.|
|Mutations Made By|| |
Dr. Tyler Jacks, Massachusetts Institute of Technology
When maintained as a live colony, homozygotes may be bred.
When using the C57BL/6-Apctm1Tyj/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #009045 in your Materials and Methods section.
|Heterozygous for Apc<tm1Tyj>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
|Frozen Mouse Embryo||$2,595.00 per straw or vial|
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