Overview

Also Known As:NOD.B10-Idd5, line R2, NOD.B10-Idd5, line R1092, NOD.B10-<Idd5R2>, 1092, R2, NOD.B10-Idd5R2

These NOD congenic strains, Stock No. 008893 and 008894, are the result of crossing segments of C57BL/10SnJ derived Chromosome 1 into the NOD/MrkTac background. These strains may be useful to better understand the variation of diabetic resistance conferred by Idd loci and should be useful for identifying diabetes susceptible candidate genes within the Idd5 subloci, specifically Idd5d.

Donating Investigator

Linda Wicker, University of Cambridge, UK

Genetic overview

Genetic Background	Generation

Idd5^C57BL/10SnJ

Allele Type	Gene Symbol	Gene Name
QTL	Idd5	insulin dependent diabetes susceptibility 5

Marker(s)

	Marker Symbol	Marker Name
	Idd5.2	insulin dependent diabetes susceptibility 5.2
	Idd5.3	insulin dependent diabetes susceptibility 5.3
	Idd5.4	insulin dependent diabetes susceptibility 5.4

View Genetics

Research Applications

Diabetes and Obesity Research
Immunology, Inflammation and Autoimmunity Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

View Price List

Details

Detailed Description

This NOD congenic strain, commonly referred to as NOD.B10-Idd5, line R2 (also known as line 1092), carries two C57BL/10SnJ (Stock No. 000666) Chromosome 1 segments. The first proximal segment is defined within D1Mit5 (63.8Mb) and D1Mit101 (147.31Mb) that includes the insulin dependent diabetes susceptibility (Idd) 5.2, 5.3 and part of the Idd5.4 loci (Idd5b, Idd5c, and Idd5d, respectively). The second distal segment is defined within D1Mit346 (152.63Mb) and D1Mit267 (157.94Mb) and contains the remainder of the Idd5.4 (Idd5d) region. This strain differs from its sister strain, NOD.B6-Idd5^C57BL/10SnJ/974MrkJ (Stock No. 008894), in that both Cd28 and Ctla4 are NOD in origin. Diabetes incidence among 200-day old female mice of this strain has been reported at 70%, compared with more than 80% in NOD controls (Hunter, et al. 2007), and males also become diabetic (incidence not done). This strain is key to understanding the variation in diabetic resistance conferred by Idd loci, in particular the epistatic interactions of Idd5.1 alleles with alleles at Idd5.4.

This strain and NOD.B10Sn-Idd5, line 974 (Stock No. 008894) together define the Idd5.1 interval.

This strain may be useful for positional mapping of the Idd5.4 loci and identifying candidate genes within the Idd5.4 region.

Development

This NOD/MrkTac congenic stock contains two genomic segments from C57BL/10SnJ introgressed into Chromosome 1. The first segment, 83.65Mb in length is defined within markers D1Mit5, 63.8Mb and D1Mit101, 147.31Mb contains Idd5.2, Idd5.3 and part of Idd5.4, a newly identified B10 diabetes susceptible region. The major candidate gene for Idd5.2 is Nramp1; and Idd5.3 is Acadl. The second, distal, segment is 5.31Mb in length and is defined within D1Mit346, 152.63Mb through D1Mit267, 157.94Mb and contains the remainder of the Idd5.4 region. Mating mice from NOD/MrkTac to C57BL/10SnJ followed by 10 additional crosses to NOD/MrkTac with selection for markers on Chromosome 1 produced this strain. Siblings carrying the C57BL/10SnJ derived segments denoted by markers D1Mit5 through D1Mit101 and D1Mit346 through D1Mit267 were intercrossed to make homozygous. 5kSNP Chip Data, based on NCBI, build 37, identifies no C57BL/10SnJ genetic contamination of other chromosomes. In 2009, the Type 1 Diabetes Resource received this strain at generation N11F35.

Control Suggestions

Additional Information

Considerations for Choosing Controls

Selected References

Hill NJ; Lyons PA; Armitage N; Todd JA; Wicker LS; Peterson LB. 2000. NOD Idd5 locus controls insulitis and diabetes and overlaps the orthologous CTLA4/IDDM12 and NRAMP1 loci in humans. Diabetes 49(10):1744-7PubMed: 11016460 MGI: J:88667
Hunter K; Rainbow D; Plagnol V; Todd JA; Peterson LB; Wicker LS. 2007. Interactions between Idd5.1/Ctla4 and other type 1 diabetes genes. J Immunol 179(12):8341-9PubMed: 18056379 MGI: J:143549
Wicker LS; Chamberlain G; Hunter K; Rainbow D; Howlett S; Tiffen P; Clark J; Gonzalez-Munoz A; Cumiskey AM; Rosa RL; Howson JM; Smink LJ; Kingsnorth A; Lyons PA; Gregory S; Rogers J; Todd JA; Peterson LB. 2004. Fine mapping, gene content, comparative sequencing, and expression analyses support Ctla4 and Nramp1 as candidates for Idd5.1 and Idd5.2 in the nonobese diabetic mouse. J Immunol 173(1):164-73PubMed: 15210771 MGI: J:90924

View All References

Genetics

Idd5^C57BL/10SnJ

Allele Symbol: Idd5^C57BL/10SnJ

Allele Name	C57BL/10SnJ
Allele Type	QTL
Allele Synonym(s)	Idd5^b10
Gene Symbol and Name	Idd5, insulin dependent diabetes susceptibility 5
Gene Synonym(s)
Strain of Origin	C57BL/10SnJ
Chromosome	1
General Note	Idd3 and Idd5 appear to interact additively. C57BL/6-derived alleles at Idd3 in combination with C57BL/10SnJ-derived alleles at Idd5 partially reverses autoimmune exocrinopathy markers on an NOD genetic background.
Molecular Note	This allele confers resistance to insulin dependent diabetes compared to NOD/MrkTac.

Idd5.2

Marker Symbol: Idd5.2

Marker Synonym(s)
Chromosome(s)	1

Idd5.3

Marker Symbol: Idd5.3

Marker Synonym(s)
Chromosome(s)	1

Idd5.4

Marker Symbol: Idd5.4

Marker Synonym(s)
Chromosome(s)	1

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
- Type 1 Diabetes (IDDM) Analysis Strains
  - NOD/ShiLtJ Non-MHC Congenics
Immunology, Inflammation and Autoimmunity Research
- Autoimmunity
  - Type 1 Diabetes

Mammalian Phenotype Terms by Genotype

References

Hill NJ; Lyons PA; Armitage N; Todd JA; Wicker LS; Peterson LB. 2000. NOD Idd5 locus controls insulitis and diabetes and overlaps the orthologous CTLA4/IDDM12 and NRAMP1 loci in humans. Diabetes 49(10):1744-7PubMed: 11016460 MGI: J:88667
Hunter K; Rainbow D; Plagnol V; Todd JA; Peterson LB; Wicker LS. 2007. Interactions between Idd5.1/Ctla4 and other type 1 diabetes genes. J Immunol 179(12):8341-9PubMed: 18056379 MGI: J:143549
Wicker LS; Chamberlain G; Hunter K; Rainbow D; Howlett S; Tiffen P; Clark J; Gonzalez-Munoz A; Cumiskey AM; Rosa RL; Howson JM; Smink LJ; Kingsnorth A; Lyons PA; Gregory S; Rogers J; Todd JA; Peterson LB. 2004. Fine mapping, gene content, comparative sequencing, and expression analyses support Ctla4 and Nramp1 as candidates for Idd5.1 and Idd5.2 in the nonobese diabetic mouse. J Immunol 173(1):164-73PubMed: 15210771 MGI: J:90924

Additional - Idd5.2 related

Additional - Idd5^C57BL/10SnJ related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Genotyping resources and troubleshooting
Breeding Considerations

Mice are maintained homozygous x homozygous siblings. Expected coat color is albino.
- Additional Breeding and Husbandry Support
Appearance
- albino
  Related Genotype: A/A Tyr^c/Tyr^c
Citation
When using the NOD.B10-Idd5R2 mouse strain in a publication, please cite the originating article(s) and include JAX stock #008893 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
> >	Homozygous for Idd5<C57BL/10J>, 1 pair minimum

Related Products and Services

Frozen Mouse Embryo	NOD.B10Sn-Idd5<C57BL/10SnJ>/1092MrkJ Frozen Embryos	$2595.00
Frozen Mouse Embryo	NOD.B10Sn-Idd5<C57BL/10SnJ>/1092MrkJ Frozen Embryos	$2595.00
Frozen Mouse Embryo	NOD.B10Sn-Idd5<C57BL/10SnJ>/1092MrkJ Frozen Embryos	$3373.50
Frozen Mouse Embryo	NOD.B10Sn-Idd5<C57BL/10SnJ>/1092MrkJ Frozen Embryos	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:NOD.B10-Idd5, line R2, NOD.B10-Idd5, line R1092, NOD.B10-<Idd5R2>, 1092, R2, NOD.B10-Idd5R2

Donating Investigator

Genetic overview

Marker(s)

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Idd5C57BL/10SnJ

Allele Symbol: Idd5C57BL/10SnJ

Idd5.2

Marker Symbol: Idd5.2

Idd5.3

Marker Symbol: Idd5.3

Idd5.4

Marker Symbol: Idd5.4

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Idd5.2 related

Additional - Idd5C57BL/10SnJ related

Genotyping Protocols

Breeding Considerations

Appearance

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Idd5^C57BL/10SnJ

Allele Symbol: Idd5^C57BL/10SnJ

Additional - Idd5^C57BL/10SnJ related