B6.129-Pick1^tm1Rlh/J

Stock number: 008891
Research areas: Neurobiology Research

Description

Mice that are homozygous for the Pick1 (protein interacting with C kinase 1) targeted mutation exhibit several deficits in synaptic plasticity in different brain regions.

Detailed description

Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous males are not fertile, however. No gene product (protein) is detected by Western blot analysis of the brain isolated from homozygous animals. Mutant mice exhibit several deficits in synaptic plasticity in different brain regions. These mice may be useful in studies of the trafficking and localization of GluR2-containing receptors and calcium-permeable AMPA receptor plasticity (CARP).

Development

LoxP sites flanking both exon3 and a neomycin cassette were introduced using (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to CMV-cre transgenic mice to delete exon 3 and the neomycin cassette. The CMV-Cre transgene was bred out from the line. This strain was backcrossed to C57BL/6J for at least 11 generations by the donating laboratory.

Allele

Symbol: Pick1^tm1Rlh
Name: targeted mutation 1, Richard L Huganir
MGI accession ID: MGI:3584144
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Pick1
Marker name: protein interacting with C kinase 1
Chromosome: 15
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: Exon 3 was deleted via cre-mediated recombination, resulting in a frame-shift mutation. Western blot of mutant brains confirmed absence of protein.
General note: Phenotypic Similarity to Human Syndrome: Globozoospermia in homozygous mice (J:149846)