These Myd88fl mice have loxP sites flanking exon 3 of the myeloid differentiation primary response gene 88 (Myd88). These mutant mice may be useful for Cre-lox studies of signal transduction, Toll-like receptor signaling and natural killer cells.
Anthony L DeFranco, University of California San Francisco (UCSF)
Genetic Background | Generation |
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N12+N2F5
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Myd88 | myeloid differentiation primary response gene 88 |
Myd88fl mice have loxP sites flanking exon 3 of the myeloid differentiation primary response gene 88 (Myd88). Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissue(s).
When bred to a strain with inducible Cre recombinase expression in dendritic cells (see Stock No. 008068 for example), this mutant mouse strain may be useful in studies of Toll-like receptor signaling during immune responses.
When bred to a strain with Cre recombinase expression in hematopoietic cells (see Stock No. 008610 for example), this mutant mouse strain may be useful in studies of Toll-like receptor signaling and natural killer cells.
When bred to a strain with Cre recombinase expression in villi and crypt cells of the small and large intestines (see Stock No. 004586 for example), this mutant mouse strain may be useful in studies of microbial intestinal flora.
The Myd88fl allele was designed by Dr. Anthony L. DeFranco (University of California San Francisco) to have loxP sites flanking exon 3 of the myeloid differentiation primary response gene 88 (Myd88) on chromosome 9. Specifically, the targeting vector placed a frt-flanked neomycin resistance cassette and loxP site upstream of exon 3, and a second loxP site just downstream of exon 3 of the myeloid differentiation primary response gene 88 locus (Myd88) on chromosome 9. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric animals were bred with Actin-FLPe mice (on a C57BL/6 congenic background; see Stock No. 005703) to achieve germline transmission and to remove the neo cassette. The resulting Myd88fl mice (retaining the loxP site flanked exon 3 and a single residual frt site) were then backcrossed to C57BL/6J for 11 generations (and the Flpe-expressing transgene was removed) prior to sending heterozygous males with black coat color to The Jackson Laboratory Repository in 2009. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Allele Name | targeted mutation 1, Anthony L DeFranco |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Myd88fl |
Gene Symbol and Name | Myd88, myeloid differentiation primary response gene 88 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 9 |
Molecular Note | Exon 3 was flanked by loxP sites by placing a FRT-flanked neo-cassette with a 3' loxP site upstream of exon 3 with an additional loxP site being placed downstream of exon 3. Founders were crossed with ACTB-FLPe mice to remove the neo selection cassette. Expression levels of Myd88 were unaffected by loxP insertion. Cre-recombination is predicted to excise exon 3 and create a null allele. |
Mutations Made By | Anthony DeFranco, University of California San Francisco (UCSF) |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Myd88fl mouse strain in a publication, please cite the originating article(s) and include JAX stock #008888 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-Type for Myd88<tm1Defr> |
Frozen Mouse Embryo | B6.129P2(SJL)-Myd88<tm1Defr>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129P2(SJL)-Myd88<tm1Defr>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129P2(SJL)-Myd88<tm1Defr>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129P2(SJL)-Myd88<tm1Defr>/J Frozen Embryo | $3373.50 |
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