B6.129S6-Del(3Cd1d2-Cd1d1)1Sbp/J

Stock number: 008881
Product name: CD1d KO
Research areas: Cancer Research, Cell Biology Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools, Virology Research

Description

CD1-deficient mice harbor a knock-out of the Cd1d1/Cd1d2 loci resulting in a lack of natural killer T (NKT) cells. These mice may be useful in studying NKT cell immunology; including Th1- and Th2-responses, antiviral and antitumor responses, asthma, various inflammatory responses, autoimmunity and peripheral tolerance.

Detailed description

Homozygous (CD1-deficient) mice are viable and fertile, with no protein expression from the targeted locus observed on antigen presenting cells (APC) by FACS analysis. Natural killer T (NKT) cells are restricted by MHC class I-like CD1 molecules expressed on APC, and because the CD1 molecule is also required for the development of NKT cells, these CD1-deficient mice selectively lack NKT cells. Because activated NKT cells normally produce interleukin-4 (IL-4), interferon-gamma, granulocyte-macrophage colony-stimulating factor, IL-2 and TNF-alpha, CD1-deficient mice may exhibit abnormal immune responses dependent upon such cytokines/chemokines. These CD1-deficient mice may be useful in studying NKT cell immunology; including Th1- and Th2-responses, antiviral and antitumor responses, asthma, various inflammatory responses, autoimmunity and peripheral tolerance.

Development

A targeting vector was designed to disrupt the CD1 locus by replacing a region of both Cd1d1 and Cd1d2 genes with a neomycin cassette. The construct was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred to C57BL/6J mice to establish the CD1-mutant colony. The donating investigator reported that these mice were backcrossed to C57BL/6J mice (see SNP note below) for 12 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were backcrossed to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, at least 1 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Allele

Symbol: Del(3Cd1d2-Cd1d1)1Sbp
Name: deletion, Chr 3, Steven P Balk 1
MGI accession ID: MGI:3611776
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Del(3Cd1d2-Cd1d1)1Sbp
Marker name: deletion, Chr3, Steven P Balk 1
Chromosome: 3
Strain of origin: 129S6/SvEvTac
Molecular note: A genomic region containing Cd1d1 and Cd1d2 genes was replaced with a single neomycin cassette by homologous recombination.