CD1-deficient mice harbor a knock-out of the Cd1d1/Cd1d2 loci resulting in a lack of natural killer T (NKT) cells. These mice may be useful in studying NKT cell immunology; including Th1- and Th2-responses, antiviral and antitumor responses, asthma, various inflammatory responses, autoimmunity and peripheral tolerance.
Mark A Exley, Beth Israel Deaconess Med Cntr (Harvard)
Genetic Background | Generation |
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N13+N2F6
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Del(3Cd1d2-Cd1d1)1Sbp | deletion, Chr3, Steven P Balk 1 |
Homozygous (CD1-deficient) mice are viable and fertile, with no protein expression from the targeted locus observed on antigen presenting cells (APC) by FACS analysis. Natural killer T (NKT) cells are restricted by MHC class I-like CD1 molecules expressed on APC, and because the CD1 molecule is also required for the development of NKT cells, these CD1-deficient mice selectively lack NKT cells. Because activated NKT cells normally produce interleukin-4 (IL-4), interferon-gamma, granulocyte-macrophage colony-stimulating factor, IL-2 and TNF-alpha, CD1-deficient mice may exhibit abnormal immune responses dependent upon such cytokines/chemokines. These CD1-deficient mice may be useful in studying NKT cell immunology; including Th1- and Th2-responses, antiviral and antitumor responses, asthma, various inflammatory responses, autoimmunity and peripheral tolerance.
A targeting vector was designed to disrupt the CD1 locus by replacing a region of both Cd1d1 and Cd1d2 genes with a neomycin cassette. The construct was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred to C57BL/6J mice to establish the CD1-mutant colony. The donating investigator reported that these mice were backcrossed to C57BL/6J mice (see SNP note below) for 12 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were backcrossed to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, at least 1 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Allele Name | deletion, Chr 3, Steven P Balk 1 |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | CD1 KO; CD1d KO; CD1d-; CD1dnull; Cd1d1/Cd1d2tm1Spb; Cd1d1/Cd1d2tm1Stst |
Gene Symbol and Name | Del(3Cd1d2-Cd1d1)1Sbp, deletion, Chr3, Steven P Balk 1 |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 3 |
Molecular Note | A genomic region containing Cd1d1 and Cd1d2 genes was replaced with a single neomycin cassette by homologous recombination. |
When maintaining a live colony, homozygous mice may be bred together.
When using the CD1d KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #008881 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Cd1d2<tm1Spb> |
Frozen Mouse Embryo | B6.129S6-Del(3Cd1d2-Cd1d1)1Sbp/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Del(3Cd1d2-Cd1d1)1Sbp/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Del(3Cd1d2-Cd1d1)1Sbp/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S6-Del(3Cd1d2-Cd1d1)1Sbp/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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