These Arg1flox mutant mice (Arg1, arginase 1, liver) may be useful in generating conditional mutations for studies of immune response to bacterial and parasitic infections and argininemia.
Peter J Murray, St. Jude Children's Research Hospital
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Arg1 | arginase, liver |
These mice possess loxP sites on either side of exons 7 and 8 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 7 and 8 deleted in the cre-expressing tissue(s).
When bred to a strain with inducible Cre recombinase expression in the myeloid cell lineages (see Stock No. 004781, for example ) or endothelial cells (see Stock No. 004128 , for example), this mutant mouse strain may be useful in studies of immune response to bacterial and parasitic infections.
A loxP site flanked targeting vector containing PGK-Neo selection cassette was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 8 of the targeted gene, and another loxP site was inserted upstream of exon 7. This construct was electroporated into C57BL/6 derived Bruce4 embryonic stem (ES) cells which were transiently transfected with a Cre recombinase vector to remove the selection cassette. ES cells that had successfully undergone Cre- mediated recombination and no longer retained the cassette but did retain the loxP-flanked exons 7 and 8 were injected in recipient blastocysts. Resulting chimeric male animals were backcrossed to wildtype C57BL/6 mice. The mice were then backcrossed to BALB/cJ mice for generations before arriving at The Jackson Laboratory.
Allele Name | targeted mutation 1, Peter J Murray |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Argfl; Arg1flox |
Gene Symbol and Name | Arg1, arginase, liver |
Gene Synonym(s) | |
Strain of Origin | B6.Cg-Thy1a |
Chromosome | 10 |
Molecular Note | A loxP site was inserted upstream of exon 7 and a loxP-flanked neomycin selection cassette was inserted downstream of exon 8. Transient expression of cre recombinase removed the neomycin cassette, leaving exons 7 and 8 floxed. These exons encode in addition to the oligomerization domain, two highly conserved aspartic acid residues that are essential to the enzymatic activity of the gene product. Correct targeting was confirmed by genomic PCR. |
Mutations Made By | Peter Murray, St. Jude Children's Research Hospital |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the C.B6-Arg1tm1Pmu/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008879 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-Type for Arg1<tm1Pmu> |
Frozen Mouse Embryo | C.B6-Arg1<tm1Pmu>/J | $2595.00 |
Frozen Mouse Embryo | C.B6-Arg1<tm1Pmu>/J | $2595.00 |
Frozen Mouse Embryo | C.B6-Arg1<tm1Pmu>/J | $3373.50 |
Frozen Mouse Embryo | C.B6-Arg1<tm1Pmu>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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