B6.129P2(129S4)-Hprt1^{tm12(Ple177-EGFP/cre)Ems}/Mmjax

Stock number: 008877
Research areas: Neurobiology Research, Research Tools

Description

These Ple177-EGFPCre;mEMS762 mice have the Ple177-EGFPCre transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase 1 (Hprt1) locus on the X chromosome. As the promoter/regulatory regions of the human regulator of G-protein signaling 16 (RGS16) gene direct expression of an EGFPCre fusion protein to hippocampus, thalamus, forebrain, cortex, brainstem, cerebellum, and retina, these Ple177-EGFPCre;mEMS762 mice may be useful in studying RGS16-expressing cells in the brain and diseases affecting the brain.

Allele

Symbol: Hprt1^{tm12(Ple177-EGFP/cre)Ems}
Name: targeted mutation 12, Elizabeth M Simpson
MGI accession ID: MGI:3835523
Generation method: Targeted
Attributes: Recombinase-expressing; Reporter
Marker symbol: Hprt1
Marker name: hypoxanthine phosphoribosyltransferase 1
Chromosome: X
Strain of origin: 129P2/OlaHsd
Expressed genes: GFP,Green Fluorescent Protein,; cre,cre recombinase,bacteriophage P1
Site of expression: Expression of an EGFPCre fusion protein is directed to hippocampus, thalamus, forebrain, cortex, brainstem, cerebellum, and retina.
Molecular note: The Ple177-EGFPCre transgene (pEMS1089) was designed with the 1302 bp Ple177 minipromoter (RGS16-C; derived from an upstream candidate regulatory regions and a subsection of the human regulator of G-protein signaling 16 (/RGS16/) promoter) upstream of a minimal F5 mutant-/frt/ site, an EGFP/Cre fusion protein (enhanced green fluorescent protein (with mutated stop) and Cre recombinase), a nuclear localization signal, a second minimal /frt/ site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2).
General note: Germ line transmission of mutant cell line mEMS762 has been established.