These Ple177-EGFPCre;mEMS762 mice have the Ple177-EGFPCre transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase (Hprt) locus on the X chromosome. As the promoter/regulatory regions of the human regulator of G-protein signaling 16 (RGS16) gene direct expression of an EGFPCre fusion protein to hippocampus, thalamus, forebrain, cortex, brainstem, cerebellum, and retina, these Ple177-EGFPCre;mEMS762 mice may be useful in studying RGS16-expressing cells in the brain and diseases affecting the brain.
Elizabeth M Simpson, Centre for Molecular Medicine & Therapeutics, University of British Columbia
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing, Reporter) | Hprt | hypoxanthine guanine phosphoribosyl transferase |
Expressed Gene | GFP, Green Fluorescent Protein, |
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Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Expression of an EGFPCre fusion protein is directed to hippocampus, thalamus, forebrain, cortex, brainstem, cerebellum, and retina. |
Allele Name | targeted mutation 12, Elizabeth M Simpson |
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Allele Type | Targeted (Recombinase-expressing, Reporter) |
Allele Synonym(s) | Hprt1tm12(mEMS762)Ems; Hprt1tm12(Ple177-EGFPcre;mEMS762)Ems; RGS16-C-EGFP/cre |
Gene Symbol and Name | Hprt, hypoxanthine guanine phosphoribosyl transferase |
Gene Synonym(s) | |
Promoter | RGS16, regulator of G protein signaling 16, human |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Expression of an EGFPCre fusion protein is directed to hippocampus, thalamus, forebrain, cortex, brainstem, cerebellum, and retina. |
Strain of Origin | 129P2/OlaHsd |
Chromosome | X |
General Note | Germ line transmission of mutant cell line mEMS762 has been established. |
Molecular Note | The Ple177-EGFPCre transgene (pEMS1089) was designed with the 1302 bp Ple177 minipromoter (RGS16-C; derived from an upstream candidate regulatory regions and a subsection of the human regulator of G-protein signaling 16 (/RGS16/) promoter) upstream of a minimal F5 mutant-/frt/ site, an EGFP/Cre fusion protein (enhanced green fluorescent protein (with mutated stop) and Cre recombinase), a nuclear localization signal, a second minimal /frt/ site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). |
Mutations Made By | Elizabeth Simpson, Centre for Molecular Medicine & Therapeutics, University of British Columbia |
The donating investigator recommends maintaining this strain by breeding heterozygous females with C57BL/6J inbred males.
When using the B6.129P2(129S4)-Hprttm12(Ple177-EGFP/cre)Ems/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32924 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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