These mice are transgenic for the A53T mutation of the human SNCA (synuclein, alpha) gene under the control of a human THY1 (thymus cell antigen 1, theta) promoter. Hemizygotes are viable and fertile and develop a Parkinson-like phenotype upon aging. Hind limb paralysis due to loss of motor neurons and a resting tremor are initially seen at about eight months of age. No Lewy body-like pathology is noted. Cell death in the spinal cord (extensive) and brain are observed.
Dr. Thomas C. Sudhof, Stanford University School of Medicine
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
These mice are transgenic for the A53T mutation of the human SNCA (synuclein, alpha) gene under the control of a human THY1 (thymus cell antigen 1, theta) promoter. Hemizygotes are viable and fertile and develop a Parkinson-like phenotype upon aging. Hind limb paralysis due to loss of motor neurons and a resting tremor are initially seen at about eight months of age. No Lewy body-like pathology is noted. Cell death in the spinal cord (extensive) and brain are observed. Expression of the transgene is 10-fold increased in the brain and 20-fold in the spinal cord.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
This transgenic strain carries the human SNCA (synuclein, alpha) gene under the control of a human THY1 (thymus cell antigen 1, theta) promoter. It is not known what strain the transgenic vector was originally injected into, but this line has been backcrossed approximately 5 times to C57BL/6 by the donating laboratory. SNP (single nucleotide polymorphism) analysis performed by The Jackson Laboratory revealed that this strain was on a mixed genetic background (eight out of 27 markers were segregating for non-C57BL/6 alleles). Further backcrossing to C57BL/6J was carried out at The Jackson Laboratory to enhance the C57BL/6 background of this line.
Expressed Gene | SNCA, synuclein alpha, human |
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Site of Expression |
Allele Name | transgene insertion F53, Thomas C Sudhof |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | F53 |
Gene Symbol and Name | Tg(THY1-SNCA*A53T)F53Sud, transgene insertion F53, Thomas C Sudhof |
Gene Synonym(s) | |
Promoter | THY1, Thy-1 cell surface antigen, human |
Expressed Gene | SNCA, synuclein alpha, human |
Strain of Origin | Not Specified |
Chromosome | UN |
Molecular Note | The transgenic construct carries the A53T mutant form of the human SNCA (synuclein, alpha) gene under the control of a human THY1 (thymus cell antigen 1, theta) promoter. Expression of the transgene is 10-fold increased in the brain and 20-fold in the spinal cord. |
Mutations Made By | Dr. Thomas Sudhof, Stanford University School of Medicine |
When maintained as a live colony, hemizygotes may be bred to wildtype mice.
When using the F53 mouse strain in a publication, please cite the originating article(s) and include JAX stock #008859 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non carrier for Tg(THY1-SNCA*A53T)F53Sud |
Frozen Mouse Embryo | B6.Cg-Tg(THY1-SNCA*A53T)F53Sud/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(THY1-SNCA*A53T)F53Sud/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(THY1-SNCA*A53T)F53Sud/J | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(THY1-SNCA*A53T)F53Sud/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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