JAX Mouse Strain Datasheet - 008851

STOCK Tg(Wnt1-cre/ERT)1Alj/J

Stock No:: 008851 |; Wnt1-CreER^T

Transgenic

Cryo Recovery

Overview

Also Known As: Wnt1-CreER^T

These transgenic mice express a tamoxifen-inducible cre recombinase directed by the wingless-related MMTV integration site 1 (Wnt1) promoter/regulatory sequences. When induced, cre activity is observed in the Wnt1-expressing cells; including midbrain, developing neural tube, migrating neural crest cells and dopaminergic neuron populations.

Donating Investigator

Dr. Alexandra L Joyner, Memorial Sloan-Kettering Cancer Center

Genetic overview

Genetic Background	Generation

Tg(Wnt1-cre/ERT)1Alj

Allele Type
Transgenic (Inducible, Recombinase-expressing)

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Research Applications

Neurobiology Research
Research Tools

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Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

Mice hemizygous for the Wnt1-CreER^T transgene are viable and fertile, with expression of a tamoxifen-inducible Cre recombinase (Cre-ER^T) directed by mouse Wnt1 promoter/enhancer elements. CreER^T fusion gene activity is inducible; observed following tamoxifen administration. As such, when Wnt1-CreER^T transgenic mice are bred with mice containing loxP-flanked sequences, tamoxifen-inducible Cre-mediated recombination will result in deletion of the floxed sequences in the Wnt1-expressing cells of the offspring. Depending upon when tamoxifen is administered, Cre recombinase activity may be observed throughout the mesencephalon (midbrain) and rhombomere 1 (~E8.5), or more restricted to the dorsal and ventral midline of the midbrain and a ring around the posterior border of the midbrain (E9.5-12.5), lateral edge of the neural plate (lower rhombic lip) in rhombomere2- rhombomere7, spinal cord and diencephalon, as well as progenitor populations giving rise to migrating neural crest cells and dopaminergic neuron populations (E9.5-12.5). The donating investigator has not determined the phenotype of homozygous mice to date (January 2012).

The Cre-ER^T fusion protein consists of Cre recombinase fused to a G521R mutant form of the human estrogen receptor binding domain which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ER^T can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered.

Development

The Wnt1-CreER^T transgene was designed with mouse Wnt1 promoter/enhancer elements (from the pWexp3 plasmid) upstream of the Cre-ER^T fusion gene (Cre recombinase fused to a G521R mutant form of the human estrogen receptor ligand binding domain). Specifically, the transgene was created by inserting the CreERT fusion gene (followed by an 825 bp untranslated LacZtag sequence to provide a tag for the transgene derived RNA) into a 10 kbp modified mouse Wnt1 genomic sequence at a polylinker site between the promoter and enhancer (the Wnt1 genomic sequence spans from approximately 1 kbp upstream of the translation initiation codon to approximately 5.5 kbp downstream of the polyA signal, and is modified with a polylinker that disrupts the translational start site). This transgene was injected into Swiss Webster zygotes. Founder mice were identified and bred to Swiss Webster mice to generate the colony. Hemizygous mice were routinely bred to Swiss Webster mice for many generations (and may also have been bred to Gt(ROSA)26Sor^tm1Sor mutant mice [on an undisclosed genetic background]) prior to sending to The Jackson Laboratory Repository. In addition, the donating investigator reports that although these mice harbor no additional mutant loci, they may contain incidental genetic background contributions as a result of past matings with other mutant mouse strains. Upon arrival, Wnt1-CreER^T transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony at The Jackson Laboratory Repository.

Expression Data

Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombinase activity is observed throughout the mesencephalon (midbrain) and rhombomere 1 (~E8.5), or more restricted to the dorsal and ventral midline of the midbrain and a ring around the posterior border of the midbrain (E9.5-12.5), lateral edge of the neural plate (lower rhombic lip) in rhombomere2- rhombomere7, spinal cord and diencephalon, as well as progenitor populations giving rise to migrating neural crest cells and dopaminergic neuron populations (E9.5-12.5).

Expressed Gene

cre, cre recombinase, bacteriophage P1

Site of Expression

Cre recombinase activity is observed throughout the mesencephalon (midbrain) and rhombomere 1 (~E8.5), or more restricted to the dorsal and ventral midline of the midbrain and a ring around the posterior border of the midbrain (E9.5-12.5), lateral edge of the neural plate (lower rhombic lip) in rhombomere2- rhombomere7, spinal cord and diencephalon, as well as progenitor populations giving rise to migrating neural crest cells and dopaminergic neuron populations (E9.5-12.5).

Control Suggestions

Noncarrier

Additional Information

Considerations for Choosing Controls

Selected References

Zervas M; Millet S; Ahn S; Joyner AL. 2004. Cell behaviors and genetic lineages of the mesencephalon and rhombomere 1. Neuron 43(3):345-57. PubMed: 15294143 MGI: J:152670

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Genetics

Tg(Wnt1-cre/ERT)1Alj

Allele Symbol: Tg(Wnt1-cre/ERT)1Alj

Allele Name	transgene insertion 1, Alexandra L Joyner
Allele Type	Transgenic (Inducible, Recombinase-expressing)
Allele Synonym(s)	Wnt1-CreER; Wnt1-CreER^T
Gene Symbol and Name	Tg(Wnt1-cre/ERT)1Alj, transgene insertion 1, Alexandra L Joyner
Gene Synonym(s)	Wnt1-CreER^T
Promoter	Wnt1, wingless-type MMTV integration site family, member 1, mouse, laboratory
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombinase activity is observed throughout the mesencephalon (midbrain) and rhombomere 1 (~E8.5), or more restricted to the dorsal and ventral midline of the midbrain and a ring around the posterior border of the midbrain (E9.5-12.5), lateral edge of the neural plate (lower rhombic lip) in rhombomere2- rhombomere7, spinal cord and diencephalon, as well as progenitor populations giving rise to migrating neural crest cells and dopaminergic neuron populations (E9.5-12.5).
Strain of Origin	Swiss Webster
Chromosome	UN
Molecular Note	cDNA encoding the cre/ERT fusion protein was inserted into a vector containing the Wnt1 enhancer. Two lines showing similar expression patterns in reporter mice were generated.
Mutations Made By	Dr. Alexandra Joyner, Memorial Sloan-Kettering Cancer Center

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Cre-lox System
  - Cre Recombinase expression in neural tissue
Research Tools
- Cre-lox System
  - Cre Recombinase Expression
  - Cre Recombinase Expression: Inducible
- Developmental Biology Research
  - Cre-lox System
  - transplantation marker for embryonic and adult tissue
- Genetics Research
  - Mutagenesis and Transgenesis
  - Mutagenesis and Transgenesis: Cre-lox System
  - Tissue/Cell Markers
  - Tissue/Cell Markers: Cre-lox System
  - Tissue/Cell Markers: transplantation marker for embryonic and adult tissue
- Neurobiology Research
  - cell marker

Genotype: cre related

Research Tools
- Cre-lox System
- Genetics Research
  - Mutagenesis and Transgenesis
  - Mutagenesis and Transgenesis: Cre-lox System

References

Zervas M; Millet S; Ahn S; Joyner AL. 2004. Cell behaviors and genetic lineages of the mesencephalon and rhombomere 1. Neuron 43(3):345-57. PubMed: 15294143 MGI: J:152670

Additional - Tg(Wnt1-cre/ERT)1Alj related

Brown A; Machan JT; Hayes L; Zervas M. 2011. Molecular organization and timing of Wnt1 expression define cohorts of midbrain dopamine neuron progenitors in vivo. J Comp Neurol 519(15):2978-3000. PubMed: 21713770 MGI: J:176481
Deckelbaum RA; Holmes G; Zhao Z; Tong C; Basilico C; Loomis CA. 2012. Regulation of cranial morphogenesis and cell fate at the neural crest-mesoderm boundary by engrailed 1. Development 139(7):1346-58. PubMed: 22395741 MGI: J:184618
Ellisor D; Koveal D; Hagan N; Brown A; Zervas M. 2009. Comparative analysis of conditional reporter alleles in the developing embryo and embryonic nervous system. Gene Expr Patterns 9(7):475-89. PubMed: 19616131 MGI: J:152409
Ellisor D; Zervas M. 2010. Tamoxifen dose response and conditional cell marking: is there control? Mol Cell Neurosci 45(2):132-8. PubMed: 20600933 MGI: J:171319
Hagan N; Zervas M. 2012. Wnt1 expression temporally allocates upper rhombic lip progenitors and defines their terminal cell fate in the cerebellum. Mol Cell Neurosci 49(2):217-29. PubMed: 22173107 MGI: J:191519
Heude E; Bellessort B; Fontaine A; Hamazaki M; Treier AC; Treier M; Levi G; Narboux-Neme N. 2015. Etiology of craniofacial malformations in mouse models of blepharophimosis, ptosis and epicanthus inversus syndrome. Hum Mol Genet 24(6):1670-81. PubMed: 25416281 MGI: J:221983
Yang J; Brown A; Ellisor D; Paul E; Hagan N; Zervas M. 2013. Dynamic temporal requirement of Wnt1 in midbrain dopamine neuron development. Development 140(6):1342-52. PubMed: 23444360 MGI: J:194842

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Hemizygous or Non carrier for Tg(Wnt1-cre/ERT)1Alj

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Also Known As: Wnt1-CreERT

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Tg(Wnt1-cre/ERT)1Alj

Allele Symbol: Tg(Wnt1-cre/ERT)1Alj

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: cre related

References

Additional - Tg(Wnt1-cre/ERT)1Alj related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Also Known As: Wnt1-CreER^T