Ctgf-Tg2-Cre mice express Cre recombinase under the control of the Ctgf (connective tissue growth factor) promoter/enhancer regions, and may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain tissues (including cortex and hippocampus).
Ed Lein, Allen Institute for Brain Science
Theresa Zwingman, Allen Institute for Brain Science
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
Hemizygous Ctgf-Tg2-Cre mice are viable and fertile, with cre expression directed to cortex and hippocampus by the Ctgf promoter/enhancer regions within the BAC transgene. The donating investigators may not have assessed expression in tissues other than brain. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These Ctgf-Tg2-Cre mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain tissues (including cortex and hippocampus).
For characterization information, see images at the Allen Institute for Brain Science website (Ctgf-Tg2-Cre images).
The 189 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-257E12, containing the entire Ctgf locus (and other genes), was modified by targeting a Cre recombinase coding sequence (the CreERT2 sequence modified back to encode the normal Cre recombinase gene), SV40 polyA signal, and frt-flanked neomycin cassette to the ATG start site of the Ctgf locus. The targeted BAC sequences were further modified using FLP recombination to remove the selection cassette and linearized to remove its original vector backbone. The resulting modified BAC was microinjected into embryos from B6C3 mice ((C57BL/6 x C3H)F1 x C57BL/6). Transgenic mice from founder line Ctgf-Tg2-Cre were subsequently backcrossed to C57BL/6J for two generations prior to arrival at The Jackson Laboratory. Upon arrival, transgenic mice were bred with C57BL/6J inbred mice for at least one additional generation to establish this colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Hemizygous Ctgf-Tg2-Cre mice are viable and fertile, with cre expression directed to cortex and hippocampus by the Ctgf promoter/enhancer regions within the BAC transgene. |
Allele Name | transgene insertion 2, Ed Lein |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | Ctgf-Tg2-Cre |
Gene Symbol and Name | Tg(Ctgf-cre)2Aibs, transgene insertion 2, Ed Lein |
Gene Synonym(s) | |
Promoter | Ccn2, cellular communication network factor 1, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Hemizygous Ctgf-Tg2-Cre mice are viable and fertile, with cre expression directed to cortex and hippocampus by the Ctgf promoter/enhancer regions within the BAC transgene. |
Strain of Origin | (C57BL/6 x C3H)F1 x C57BL/6 |
Chromosome | UN |
Molecular Note | The 189 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-257E12, containing the entire Ctgf locus (and other genes), was modified by targeting a Cre recombinase coding sequence (the CreERT2 sequence modified back to encode the normal Cre recombinase gene), SV40 polyA signal, and frt-flanked neomycin cassette to the ATG start site of the Ctgf locus. The targeted BAC sequences were further modified using FLP recombination to remove the selection cassette and linearized to remove its original vector backbone. Cre expression is directed to cortex and hippocampus by the Ctgf promoter/enhancer regions within the BAC transgene. |
Mutations Made By | Ed Lein, Allen Institute for Brain Science |
When maintaining a live colony, transgenic carrier mice may be bred together, to wildtype (noncarrier) siblings, or to C57BL/6J inbred mice (Stock No. 000664).
When using the Ctgf-Tg2-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #008844 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non Carrier forTg(Ctgf-cre)2Aibs |
Frozen Mouse Embryo | B6;C3-Tg(Ctgf-cre)2Aibs/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;C3-Tg(Ctgf-cre)2Aibs/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;C3-Tg(Ctgf-cre)2Aibs/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;C3-Tg(Ctgf-cre)2Aibs/J Frozen Embryo | $3373.50 |
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