B6;C3-Tg(Ctgf-cre)2Aibs/J

Stock number: 008844
Product name: Ctgf-Tg2-Cre
Research areas: Neurobiology Research, Research Tools

Description

Ctgf-Tg2-Cre mice express Cre recombinase under the control of the Ctgf (connective tissue growth factor) promoter/enhancer regions, and may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain tissues (including cortex and hippocampus).

Detailed description

Hemizygous Ctgf-Tg2-Cre mice are viable and fertile, with cre expression directed to cortex and hippocampus by the Ctgf promoter/enhancer regions within the BAC transgene. The donating investigators may not have assessed expression in tissues other than brain. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These Ctgf-Tg2-Cre mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain tissues (including cortex and hippocampus).

For characterization information, see images at the Allen Institute for Brain Science website (Ctgf-Tg2-Cre images).

Development

The 189 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-257E12, containing the entire Ctgf locus (and other genes), was modified by targeting a Cre recombinase coding sequence (the CreERT2 sequence modified back to encode the normal Cre recombinase gene), SV40 polyA signal, and frt-flanked neomycin cassette to the ATG start site of the Ctgf locus. The targeted BAC sequences were further modified using FLP recombination to remove the selection cassette and linearized to remove its original vector backbone. The resulting modified BAC was microinjected into embryos from B6C3 mice ((C57BL/6 x C3H)F1 x C57BL/6). Transgenic mice from founder line Ctgf-Tg2-Cre were subsequently backcrossed to C57BL/6J for two generations prior to arrival at The Jackson Laboratory. Upon arrival, transgenic mice were bred with C57BL/6J inbred mice for at least one additional generation to establish this colony.

Allele

Symbol: Tg(Ctgf-cre)2Aibs
Name: transgene insertion 2, Ed Lein
MGI accession ID: MGI:3838494
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Ctgf-cre)2Aibs
Marker name: transgene insertion 2, Ed Lein
Chromosome: UN
Strain of origin: (C57BL/6 x C3H)F1 x C57BL/6
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Hemizygous Ctgf-Tg2-Cre mice are viable and fertile, with cre expression directed to cortex and hippocampus by the Ctgf promoter/enhancer regions within the BAC transgene.
Molecular note: The 189 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-257E12, containing the entire Ctgf locus (and other genes), was modified by targeting a Cre recombinase coding sequence (the CreERT2 sequence modified back to encode the normal Cre recombinase gene), SV40 polyA signal, and frt-flanked neomycin cassette to the ATG start site of the Ctgf locus. The targeted BAC sequences were further modified using FLP recombination to remove the selection cassette and linearized to remove its original vector backbone. Cre expression is directed to cortex and hippocampus by the Ctgf promoter/enhancer regions within the BAC transgene.