These "VPAC2 R TG mice" (or VPAC2 Tg mice) have the murine lymphocyte protein tyrosine kinase (Lck) proximal promoter directing persistently high expression of huVPAC2R (human vasoactive intestinal peptide G-protein-coupled receptor 2 [VIPR2 primarily to CD4+ T cells at levels typically observed only after maximal TCR stimulation, and may be useful as a Th2-bias mouse model for studying innate and adaptive immunity, T cell and macrophage function, allergy and inflammation, and cytokine secretion, as well as immunopathologies related to Th1/Th2 cell ratio imbalance and neuroendocrine/neuroactive peptides in immune function.
Edward J Goetzl, Univ of California Medical Center (UCSF)
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
These "VPAC2 R TG mice" (or VPAC2R Tg mice) are viable and fertile, harboring the LCK-huVPAC2 transgene. This transgene has the murine lymphocyte protein tyrosine kinase (Lck) proximal promoter directing persistently high expression of huVPAC2R (human vasoactive intestinal peptide G-protein-coupled receptor 2 [VIPR2 or VIPR2]) primarily to CD4+ T cells (T-helper/inducer cells). As such, expression of huVPAC2R mRNA and protein is highest in spleen and thymus, but also observed in other tissues at much lower levels. The constitutive high huVPAC2R expression on CD4+ T cells is characteristic of levels usually observed only after maximal TCR stimulation. Transgenic mice have elevated blood IgE, IgG1, and eosinophil levels. The effector T cell phenotype is altered; with Th2-bias cytokine profiles (increased IL-4, IL-5, c-maf and junB) associated with altered hypersensitivity states (increased immediate-type hypersensitivity/decreased delayed-type hypersensitivity). Expression of huVPAC2R does not significantly alter the number or composition of splenic T cells. Although transgenic mice have more IL-4-secreting CD4+ T cells from splenic preparations compared with wildtype mice, the amount of IL-4 secreted per cell is not altered. These VPAC2 R TG mice may be useful as a Th2-bias mouse model for studying innate and adaptive immunity, T cell and macrophage function, allergy and inflammation, and cytokine secretion, as well as immunopathologies related to Th1/Th2 cell ratio imbalance and neuroendocrine/neuroactive peptides in immune function.
The 4.6 kb LCK-huVPAC2 transgene was designed with a murine lymphocyte protein tyrosine kinase (Lck) proximal promoter and full-length human VPAC2R cDNA (huVPAC2 R or VIPR2; vasoactive intestinal peptide G-protein-coupled receptor 2). This transgene was microinjected into C57BL/6 oocytes, and a male founder animal (founder line unspecified) was bred to C57BL/6 females to establish the transgenic colony. This colony was then maintained by breeding high CD4+ T cell VPAC2R expressing transgenic mice together for many generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred with C57BL/6J inbred mice (Stock No. 000664) to establish the colony.
Expressed Gene | VIPR2, vasoactive intestinal peptide receptor 2, human |
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Site of Expression |
Allele Name | transgene insertion 1, Edward J Goetzl |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | huVPAC2 R; LCK-huVPAC2; LCK-huVPAC2R |
Gene Symbol and Name | Tg(Lck-VIPR2)1Ejg, transgene insertion 1, Edward J Goetzl |
Gene Synonym(s) | |
Promoter | Lck, lymphocyte protein tyrosine kinase, mouse, laboratory |
Expressed Gene | VIPR2, vasoactive intestinal peptide receptor 2, human |
Strain of Origin | C57BL/6 |
Chromosome | UN |
Molecular Note | A murine lymphocyte protein tyrosine kinase (Lck) proximal promoter was used to drive the full-length human VPAC2R cDNA (huVPAC2 R or VIPR2; vasoactive intestinal peptide G-protein-coupled receptor 2). This transgene was microinjected into C57BL/6 oocytes, and a male founder animal was bred to C57BL/6 females to establish the line. |
Mutations Made By | Edward Goetzl, Univ of California Medical Center (UCSF) |
When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice. The donating investigator reports breeding homozygous mice together.
When using the C57BL/6-Tg(Lck-VIPR2)1Ejg/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008842 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous for Tg(Lck-VIPR2)1Ejg |
Frozen Mouse Embryo | C57BL/6-Tg(Lck-VIPR2)1Ejg/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Lck-VIPR2)1Ejg/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Lck-VIPR2)1Ejg/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Tg(Lck-VIPR2)1Ejg/J Frozen Embryo | $3373.50 |
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