B6.Cg-Tg(Prdm1-cre)1Masu/J

Stock number: 008827
Research areas: Research Tools

Description

Prdm1-Cre (Blimp1-Cre) transgenic mice express Cre recombinase under the control of the mouse Prdm1 promoter. These mice may be useful for generating tissue-specific targeted mutants for studies of development and germ cell fate.

Heterozygotes are slow to produce litters. The litters are small and it is not uncommon to lose pups before weaning. Heterozygote x wildtype (or C57BL/6J, Stock No. 000664) crosses are recommended to maintain this strain. Given the breeding/viability challenges, homozygotes will not be produced in the JAX colony. (February, 2020).

Hair loss, likely attributable to the C57BL/6 genetic background, is also observed.

Detailed description

Prdm1-Cre (Blimp1-Cre) transgenic mice express Cre recombinase under the control of the mouse Prdm1 (PR domain containing 1, with ZNF domain; Blimp1) promoter. Cre-mediated recombination is detected in 55-76% of primordial germ cells when this strain is crossed with Gt(ROSA)26-GFP reporter mice. Expression is also seen in plasma cells. These mice may be useful for generating tissue-specific targeted mutants for studies of development and germ cell fate.

Heterozygotes are slow to produce litters. The litters are small and it is not uncommon to lose pups before weaning. Heterozygote x wildtype (or C57BL/6J, Stock No. 000664) crosses are recommended to maintain this strain. Given the breeding/viability challenges, homozygotes will not be produced in the JAX colony. (February, 2020).

Hair loss, likely attributable to the C57BL/6 genetic background, is also observed.

Development

The exon 1 coding region of the targeted mouse gene was substituted with the first 100bp of the Cre sequence preceded by a nuclear localization sequence (NLS). Exon 2 was replaced by the remaining Cre sequence followed by a transcriptional stop cassette. The modified BAC vector was linearized and microinjected into pronuclei of B6CBAF1 zygotes. This strain was backcrossed to C57BL/6 four times by the donating investigator prior to sending to The Jackson Laboratory in 2009. Upon arrival, at least one additional backcross to C57BL/6J was performed.

Allele

Symbol: Tg(Prdm1-cre)1Masu
Name: transgene insertion 1, M Azim Surani
MGI accession ID: MGI:3586890
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Prdm1-cre)1Masu
Marker name: transgene insertion 1, M Azim Surani
Chromosome: UN
Strain of origin: (C57BL/6 x CBA)F1
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Cre-mediated recombination is detected in 55-76% of primordial germ cells when this mice carryint this transgene are crossed with Gt(ROSA)26-GFP reporter mice.
Molecular note: The coding region in exon 1 and 2 were replaced by an NLS and Cre cDNA sequence, followed by a transcriptional STOP cassette.