Overview

Prdm1-Cre (Blimp1-Cre) transgenic mice express Cre recombinase under the control of the mouse Prdm1 promoter. These mice may be useful for generating tissue-specific targeted mutants for studies of development and germ cell fate.

Heterozygotes are slow to produce litters. The litters are small and it is not uncommon to lose pups before weaning. Heterozygote x wildtype (or C57BL/6J, Stock No. 000664) crosses are recommended to maintain this strain. Given the breeding/viability challenges, homozygotes will not be produced in the JAX colony. (February, 2020).

Hair loss, likely attributable to the C57BL/6 genetic background, is also observed.

Donating Investigator

Michel C Nussenzweig, The Rockefeller University

Genetic overview

Genetic Background	Generation

Tg(Prdm1-cre)1Masu

Allele Type
Transgenic (Recombinase-expressing)

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Research Applications

Research Tools

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Prdm1-Cre (Blimp1-Cre) transgenic mice express Cre recombinase under the control of the mouse Prdm1 (PR domain containing 1, with ZNF domain; Blimp1) promoter. Cre-mediated recombination is detected in 55-76% of primordial germ cells when this strain is crossed with Gt(ROSA)26-GFP reporter mice. Expression is also seen in plasma cells. These mice may be useful for generating tissue-specific targeted mutants for studies of development and germ cell fate.

Heterozygotes are slow to produce litters. The litters are small and it is not uncommon to lose pups before weaning. Heterozygote x wildtype (or C57BL/6J, Stock No. 000664) crosses are recommended to maintain this strain. Given the breeding/viability challenges, homozygotes will not be produced in the JAX colony. (February, 2020).

Hair loss, likely attributable to the C57BL/6 genetic background, is also observed.

Development

The exon 1 coding region of the targeted mouse gene was substituted with the first 100bp of the Cre sequence preceded by a nuclear localization sequence (NLS). Exon 2 was replaced by the remaining Cre sequence followed by a transcriptional stop cassette. The modified BAC vector was linearized and microinjected into pronuclei of B6CBAF1 zygotes. This strain was backcrossed to C57BL/6 four times by the donating investigator prior to sending to The Jackson Laboratory in 2009. Upon arrival, at least one additional backcross to C57BL/6J was performed.

Expression Data

Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre-mediated recombination is detected in 55-76% of primordial germ cells when this mice carryint this transgene are crossed with Gt(ROSA)26-GFP reporter mice.

Control Suggestions

Noncarrier
000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Ohinata Y; Payer B; O'Carroll D; Ancelin K; Ono Y; Sano M; Barton SC; Obukhanych T; Nussenzweig M; Tarakhovsky A; Saitou M; Surani MA. 2005. Blimp1 is a critical determinant of the germ cell lineage in mice. Nature 436(7048):207-13PubMed: 15937476 MGI: J:99994

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Genetics

Tg(Prdm1-cre)1Masu

Allele Symbol: Tg(Prdm1-cre)1Masu

Allele Name	transgene insertion 1, M Azim Surani
Allele Type	Transgenic (Recombinase-expressing)
Allele Synonym(s)	Blimp1Cre; Blimp1-Cre
Gene Symbol and Name	Tg(Prdm1-cre)1Masu, transgene insertion 1, M Azim Surani
Gene Synonym(s)
Promoter	Prdm1, PR domain containing 1, with ZNF domain, mouse, laboratory
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre-mediated recombination is detected in 55-76% of primordial germ cells when this mice carryint this transgene are crossed with Gt(ROSA)26-GFP reporter mice.
Strain of Origin	(C57BL/6 x CBA)F1
Chromosome	UN
Molecular Note	The coding region in exon 1 and 2 were replaced by an NLS and Cre cDNA sequence, followed by a transcriptional STOP cassette.
Mutations Made By	M. Azim Surani, Gurdon Institute,University of Cambridge

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Cre-lox System
  - Cre Recombinase Expression
- Developmental Biology Research
  - Cre-lox System

Mammalian Phenotype Terms by Genotype

References

Ohinata Y; Payer B; O'Carroll D; Ancelin K; Ono Y; Sano M; Barton SC; Obukhanych T; Nussenzweig M; Tarakhovsky A; Saitou M; Surani MA. 2005. Blimp1 is a critical determinant of the germ cell lineage in mice. Nature 436(7048):207-13PubMed: 15937476 MGI: J:99994

Additional - Tg(Prdm1-cre)1Masu related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- QPCR:Generic Cre Quantitative PCR
- Standard PCR:Tg(Prdm1-cre)
- Standard PCR:Tg(Prdm1-cre)
- Genotyping resources and troubleshooting
Breeding Considerations

Heterozygotes are slow to produce litters. The litters are small and it is not uncommon to lose pups before weaning. Heterozygote x wildtype (or C57BL/6J, Stock No. 000664) crosses are recommended to maintain this strain. Given the breeding/viability challenges, homozygotes will not be produced in the JAX colony. (February, 2020).
Hair loss, likely attributable to the C57BL/6 genetic background, is also observed.
- Additional Breeding and Husbandry Support
Mating System
- Noncarrier x Hemizygote
- Hemizygote x Noncarrier
Citation
When using the B6.Cg-Tg(Prdm1-cre)1Masu/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008827 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
> >	Hemizygous or non-carrier for Tg(Prdm1-cre)1Masu

Related Products and Services

Frozen Mouse Embryo	B6.Cg-Tg(Prdm1-cre)1Masu/J	$2595.00
Frozen Mouse Embryo	B6.Cg-Tg(Prdm1-cre)1Masu/J	$2595.00
Frozen Mouse Embryo	B6.Cg-Tg(Prdm1-cre)1Masu/J	$3373.50
Frozen Mouse Embryo	B6.Cg-Tg(Prdm1-cre)1Masu/J	$3373.50

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Tg(Prdm1-cre)1Masu

Allele Symbol: Tg(Prdm1-cre)1Masu

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Tg(Prdm1-cre)1Masu related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information