Prdm1-Cre (Blimp1-Cre) transgenic mice express Cre recombinase under the control of the mouse Prdm1 promoter. These mice may be useful for generating tissue-specific targeted mutants for studies of development and germ cell fate.
Heterozygotes are slow to produce litters. The litters are small and it is not uncommon to lose pups before weaning. Heterozygote x wildtype (or C57BL/6J, Stock No. 000664) crosses are recommended to maintain this strain. Given the breeding/viability challenges, homozygotes will not be produced in the JAX colony. (February, 2020).
Hair loss, likely attributable to the C57BL/6 genetic background, is also observed.
Michel C Nussenzweig, The Rockefeller University
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
Prdm1-Cre (Blimp1-Cre) transgenic mice express Cre recombinase under the control of the mouse Prdm1 (PR domain containing 1, with ZNF domain; Blimp1) promoter. Cre-mediated recombination is detected in 55-76% of primordial germ cells when this strain is crossed with Gt(ROSA)26-GFP reporter mice. Expression is also seen in plasma cells. These mice may be useful for generating tissue-specific targeted mutants for studies of development and germ cell fate.
Heterozygotes are slow to produce litters. The litters are small and it is not uncommon to lose pups before weaning. Heterozygote x wildtype (or C57BL/6J, Stock No. 000664) crosses are recommended to maintain this strain. Given the breeding/viability challenges, homozygotes will not be produced in the JAX colony. (February, 2020).
Hair loss, likely attributable to the C57BL/6 genetic background, is also observed.
The exon 1 coding region of the targeted mouse gene was substituted with the first 100bp of the Cre sequence preceded by a nuclear localization sequence (NLS). Exon 2 was replaced by the remaining Cre sequence followed by a transcriptional stop cassette. The modified BAC vector was linearized and microinjected into pronuclei of B6CBAF1 zygotes. This strain was backcrossed to C57BL/6 four times by the donating investigator prior to sending to The Jackson Laboratory in 2009. Upon arrival, at least one additional backcross to C57BL/6J was performed.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Cre-mediated recombination is detected in 55-76% of primordial germ cells when this mice carryint this transgene are crossed with Gt(ROSA)26-GFP reporter mice. |
Allele Name | transgene insertion 1, M Azim Surani |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | Blimp1Cre; Blimp1-Cre |
Gene Symbol and Name | Tg(Prdm1-cre)1Masu, transgene insertion 1, M Azim Surani |
Gene Synonym(s) | |
Promoter | Prdm1, PR domain containing 1, with ZNF domain, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre-mediated recombination is detected in 55-76% of primordial germ cells when this mice carryint this transgene are crossed with Gt(ROSA)26-GFP reporter mice. |
Strain of Origin | (C57BL/6 x CBA)F1 |
Chromosome | UN |
Molecular Note | The coding region in exon 1 and 2 were replaced by an NLS and Cre cDNA sequence, followed by a transcriptional STOP cassette. |
Mutations Made By | M. Azim Surani, Gurdon Institute,University of Cambridge |
Heterozygotes are slow to produce litters. The litters are small and it is not uncommon to lose pups before weaning. Heterozygote x wildtype (or C57BL/6J, Stock No. 000664) crosses are recommended to maintain this strain. Given the breeding/viability challenges, homozygotes will not be produced in the JAX colony. (February, 2020).
Hair loss, likely attributable to the C57BL/6 genetic background, is also observed.
When using the B6.Cg-Tg(Prdm1-cre)1Masu/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008827 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or non-carrier for Tg(Prdm1-cre)1Masu |
Frozen Mouse Embryo | B6.Cg-Tg(Prdm1-cre)1Masu/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Prdm1-cre)1Masu/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Prdm1-cre)1Masu/J | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Prdm1-cre)1Masu/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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