The cytoplasmic domain of the targeted Cd79b antigen(Igβ) gene was replaced with the cytoplasmic domain of the Cd79a antigen (Igα) gene. Mutant B cells are able to complete all stages of development, but fail to differentiate into B1a cells. Igβc-α cells are anergic to T-independent and T-dependent antigens in vivo. This strain may be useful in studies of B cell development.
Michel C Nussenzweig, The Rockefeller University
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted | Cd79b | CD79B antigen |
The cytoplasmic domain of the targeted Igβ gene was replaced with the cytoplasmic domain of the Igα gene. Mutant B cells are able to complete all stages of development, but fail to differentiate into B1a cells. Igβc-α cells are anergic to T-independent and T-dependent antigens in vivo. This strain may be useful in studies of B cell development.
Positions 183-228 of the Igβ/Cd79b gene were replaced with a cytoplasmic tail segment (positions 161-220) of the Igα/Cd79a gene followed by a TGA stop codon. A loxP-flanked neomycin resistance cassette placed 5' of the targeted mutation was later excised with Cre. A 129/Sv-derived embryonic stem cell line was used to create the mutation. This line was backcrossed three times to C57BL/6 by the donating laboratory.
Allele Name | targeted mutation 3, Michel C Nussenzweig |
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Allele Type | Targeted |
Allele Synonym(s) | |
Gene Symbol and Name | Cd79b, CD79B antigen |
Gene Synonym(s) | |
Strain of Origin | 129/Sv |
Chromosome | 11 |
Molecular Note | The cytoplasmic tail from amino acid position 183 to 228 was replaced with the cytoplasmic tail of Cd79a from amino acid position 161 to 220. A stop codon follows the last amino acid of the inserted Cd79a segment. A floxed neo cassette was inserted downstream of the Ig domain and subsequently removed by cre-mediated recombination. The presence of the modified protein and the absence of the full length endogenous transcript was confirmed by western blot analysis on B cell lysates. |
Mutations Made By | Michel Nussenzweig, The Rockefeller University |
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the B6;129-Cd79btm3Mnz/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008822 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Cd79b<tm3Mnz> |
Frozen Mouse Embryo | B6;129-Cd79b<tm3Mnz>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Cd79b<tm3Mnz>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Cd79b<tm3Mnz>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129-Cd79b<tm3Mnz>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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