Developing B cells in these CD79B antigen (Igβ AA) targeted mutant mice have higher levels of surface IgM and IgD on immature and recirculating B cells than wildtype mice. They also show a five-fold reduction in the number of B1a B cells in the peritoneal cavity.
Michel C Nussenzweig, The Rockefeller University
Cytoplasmic tyrosines 195 and 206, associated with immune receptor tyrosine activation motifs (ITAMs), were replaced with alanine in this Ig beta targeted mutant strain. The only reported reproducible differences in developing B cells in these Igβ AA mutant mice is a higher level of surface IgM and IgD on immature and recirculating B cells. Igβ AA mice also show a five-fold reduction in the number of B1a B cells in the peritoneal cavity. This strain may be useful in studies of B cell receptor signaling.
TAT (tyrosine) codons at positions 195 and 206 in the cytoplasmic tail of the gene were replaced with GCT (alanine). A loxP-flanked neomycin-resistance gene was used for positive selection. The mutation was created in 129/Sv-derived embryonic stem cells. The loxP segment was excised through crosses with an EIIa promoter germline Cre strain on a C57BL/6 background. This line was backcrossed to C57BL/6 three times by the donating laboratory.
|Allele Name||targeted mutation 1, Anna Gazumyan|
|Gene Symbol and Name||Cd79b, CD79B antigen|
|Promoter||Cd79b, CD79B antigen, mouse, laboratory|
|Strain of Origin||129/Sv|
|Molecular Note||Tyrosines 195 and 206 were replaced with alanines. A floxed neo was included in the vector and subsequently removed via cre mediated recombination.|
|Mutations Made By|| |
Anna Gazumyan, The Rockefeller University
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the B6;129-Cd79btm1Gzmn/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008821 in your Materials and Methods section.