These f(α3) mutant mice harbor loxP sites flanking exons 11-18 of the Itga3 (integrin alpha 3 (or alpha3-integrin (α3-integrin)) gene and may be useful in generating conditional mutations for studying the role of Itga3 transmembrane cell adhesion receptors in neuronal functions in the developing and adult central nervous system, including synaptic plasticity and memory formation.
Chi-Shing Chan, Baylor College of Medicine
Mice homozygous for this f(α3) conditional allele are viable and fertile, with loxP sites flanking exons 11-18 of the Itga3 (integrin alpha 3 (or alpha3-integrin (α3-integrin)) gene. When bred to mice that express Cre recombinase, the resulting offspring will have the floxed region deleted in the cre-expressing tissue(s): such deletion leads to a non-sense mutation from direct splicing of exon 10 to exon 19 and results in a truncated peptide that is predicted to be missing more than half of the wild-type sequence, including those that encode the transmembrane and the cytoplasmic domains. These f(α3) mutant mice may be useful in generating conditional mutations for studying the role of Itga3 transmembrane cell adhesion receptors in neuronal functions in the developing and adult central nervous system, including synaptic plasticity and memory formation.
When bred to a strain expressing Cre recombinase in the hippocampal CA1 pyramidal cells (see Stock No. 005359 for example), this mutant mouse strain may be useful in studies of long term potentiation and memory.
A targeting vector was designed to place a loxP site just upstream of exon 11, and a loxP-flanked TK-neo cassette just downstream of exon 18 of the targeted gene. The construct was electroporated into 129S7/SvEvBrd-Hprt1b-m2-derived AB2.1 embryonic stem (ES) cells. Correctly targeted ES cells were then transiently transfected with a Cre recombinase-expressing plasmid. The resulting ES cells with the f(α3) genotype (TK-neo selection cassette removed; leaving a single loxP site upstream of exon 11 and a single loxP site downstream of exon 18) were injected into recipient blastocysts. Chimeric males were bred with C57BL/6J females. These f(α3) mutant mice were then backcrossed to C57BL/6J mice for 10 generations, and then maintained as homozygotes prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred with C57BL/6J for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Ronald Davis|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Itga3, integrin alpha 3|
|Strain of Origin||129S7/SvEvBrd-Hprtb-m2|
|Molecular Note||A loxP site was inserted upstream of exon 11 and a floxed neo cassette was inserted downstream of exon 18. Transient cre expression in ES cells removed the floxed neo cassette.|
|Mutations Made By|| |
Ronald Davis, The Scripps Research Institute Florida
When maintaining a live colony, homozygous mice may be bred together.
When using the B6.129S7-Itga3tm1Rdav/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008818 in your Materials and Methods section.
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