Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase (iCre, improved cre) under the control of the mouse kidney androgen regulated protein (Kap).
When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the targeted gene in the proximal tubule cells of the kidney. The Donating Investigator reports that both male and female transgenic mice, particularly on the C57BL/6J background, need to be induced with exogenous androgen. The introduction of a 10 mg subcutaneous testosterone pellet is said to result in a modest level of transgene induction 10 days after implantation.
