B6.Cg-Tg(Kap-icre)29066/2Sig/J

Stock number: 008781
Product name: KAP2-iCre
Research areas: Research Tools

Description

These transgenic mice express improved Cre recombinase (iCre) under the control of the mouse kidney androgen regulated protein (Kap). Transgene induction occurs with endogenous androgen (testosterone) treatment. Cre recombinase expression is detected in the proximal tubule cells of the renal cortex in male mice. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the targeted gene in the proximal tubule cells of the kidney.

Detailed description

Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase (iCre, improved cre) under the control of the mouse kidney androgen regulated protein (Kap).

When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the targeted gene in the proximal tubule cells of the kidney. The Donating Investigator reports that both male and female transgenic mice, particularly on the C57BL/6J background, need to be induced with exogenous androgen. The introduction of a 10 mg subcutaneous testosterone pellet is said to result in a modest level of transgene induction 10 days after implantation.

Development

A transgenic construct containing cre coding sequence (iCre, improved cre) under the control of the mouse kidney androgen regulated protein (Kap) was injected into fertilized C57BL/6J X SJL/J mouse eggs. The resulting founder mice were backcrossed for 6 generations on the C57BL/6J background.

Allele

Symbol: Tg(Kap-icre)29066/2Sig
Name: transgene insertion 29066/2, Curt Sigmund
MGI accession ID: MGI:3806307
Generation method: Transgenic
Attributes: Recombinase-expressing; Inducible
Synonyms: KAP2-iCre; Tg(Kap-cre)29066/2Sig
Marker symbol: Tg(Kap-icre)29066/2Sig
Marker name: transgene insertion 29066/2, Curt Sigmund
Marker synonyms: KAP2-iCre; Tg(Kap-cre)29066/2Sig
Chromosome: UN
Strain of origin: C57BL/6J x SJL
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: cre is expressed in male kidneys with some expression in the brain, liver and heart.
Molecular note: The Kap promoter and the distal regulatory sequences of the human angiotensinogen gene (AGT), which together confer androgen responsiveness, is used to drive expression of androgen-inducible improved Cre (iCre) in the kidney. The icre cDNA was cloned downstream of the Kap promoter (nucleotides -1542 through -1 relative to the transcription initiation site) into the NotI site of the pKAP2 vector. This is followed by the last 42 bp of human AGT exon 2 through exon 5, which lost their coding potential owing to the lack of the first 817 bp of exon 2 that included the ATG start site. Twenty-six founders were created of which only line 29066/2 expressed cre in male kidneys with some expression in the brain, liver, and heart.