These transgenic mice express improved Cre recombinase (iCre) under the control of the mouse kidney androgen regulated protein (Kap). Transgene induction occurs with endogenous androgen (testosterone) treatment. Cre recombinase expression is detected in the proximal tubule cells of the renal cortex in male mice. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the targeted gene in the proximal tubule cells of the kidney.
Dr. Curt Sigmund, University of Iowa College of Medicine
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase (iCre, improved cre) under the control of the mouse kidney androgen regulated protein (Kap).
When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the targeted gene in the proximal tubule cells of the kidney. The Donating Investigator reports that both male and female transgenic mice, particularly on the C57BL/6J background, need to be induced with exogenous androgen. The introduction of a 10 mg subcutaneous testosterone pellet is said to result in a modest level of transgene induction 10 days after implantation.
A transgenic construct containing cre coding sequence (iCre, improved cre) under the control of the mouse kidney androgen regulated protein (Kap) was injected into fertilized C57BL/6J X SJL/J mouse eggs. The resulting founder mice were backcrossed for 6 generations on the C57BL/6J background.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | cre is expressed in male kidneys with some expression in the brain, liver and heart. |
Allele Name | transgene insertion 29066/2, Curt Sigmund |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | KAP2-iCre; Tg(Kap-cre)29066/2Sig |
Gene Symbol and Name | Tg(Kap-icre)29066/2Sig, transgene insertion 29066/2, Curt Sigmund |
Gene Synonym(s) | |
Promoter | Kap, kidney androgen regulated protein, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | cre is expressed in male kidneys with some expression in the brain, liver and heart. |
Strain of Origin | C57BL/6J x SJL |
Chromosome | UN |
Molecular Note | The Kap promoter is used to drive androgen inducible improved cre (crei) in the kidney. Twenty-six founders were created of which only line 29066/2 expressed cre in male kidneys with some expression in the brain, liver and heart. |
Mutations Made By | Dr. Curt Sigmund, University of Iowa College of Medicine |
When maintaining a live colony, these mice can be bred as hemizygotes. The Donating Investigator has not attempted to make the strain homozygous.
When using the KAP2-iCre mouse strain in a publication, please cite the originating article(s) and include JAX stock #008781 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non-carrier for Tg(Kap-icre)29066/2Sig |
Frozen Mouse Embryo | B6.Cg-Tg(Kap-icre)29066/2Sig/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Kap-icre)29066/2Sig/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Kap-icre)29066/2Sig/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Kap-icre)29066/2Sig/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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