B6.129P2-Runx1^tm1Tani/J

Stock number: 008772
Research areas: Research Tools

Description

Exon 4 of these Runx1 (runt related transcription factor 1) targeted mutant mice is flanked by loxP sites. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. When crossed to a CD4-cre mouse strain, progeny have fewer CD4⁺ T cells than wildtype mice and completely lack NK T cells.

Detailed description

Exon 4 of these targeted mutant mice is flanked by loxP sites. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. When crossed to a CD4-cre mouse strain, progeny have fewer CD4⁺ T cells than wildtype mice and completely lack NK T cells.

Development

A loxP site was placed 5' of exon 4 and a loxP-flanked neomycin cassette was placed in intron 4 of the targeted gene. Transient infection with Cre excised the neomycin cassette, leaving a loxP-flanked exon 4. This mutation was created in 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. This strain was backcrossed 13 times to C57BL/6 (see SNP note below) by the donating laboratory.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.

Allele

Symbol: Runx1^tm1Tani
Name: targeted mutation 1, Ichiro Taniuchi
MGI accession ID: MGI:2447630
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Runx1
Marker name: runt related transcription factor 1
Chromosome: 16
Strain of origin: 129P2/OlaHsd
Molecular note: Exon 4 was floxed by insertion of a single upstream loxP site and a downstream floxed neo cassette. The neo cassette was subsequently removed by transient expression of cre recombinase in correctly targeted ES cells, leaving exon 4 flanked by loxP sites.