Overview

Green fluorescent protein (GFP) substituted for a portion of exon 1 replaces expression of the Gpr15 (G protein-coupled receptor 15) gene in lymphocytes of the large intestine lamina propria.

Donating Investigator

Dr. Dan R. Littman, New York University Medical Center

Genetic overview

Genetic Background	Generation

Gpr15^tm1.1Litt

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter, Null/Knockout)	Gpr15	G protein-coupled receptor 15

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Research Applications

Research Tools
Neurobiology Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Green fluorescent protein (GFP) substituted for a portion of exon 1 replaces expression of the gene in lymphocytes of the large intestine lamina propria. Homozygotes are viable and fertile.

Development

Part of exon 1 was replaced by enhanced green fluorescent protein (EGFP) and SV40polyA followed by a loxP-flanked neomycin resistance cassette. This mutation was created using a 129P2/OlaHsd-derived E14 embryonic stem (ES) cell line. The neomycin cassette was removed by crossing mice with CMV-Cre mice on a BALB/c background. This strain was backcrossed to C57BL/6 for 3 generations by the donating laboratory.

Expression Data

Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	EGFP is expressed in lymphocytes of the large intestine lamina propria.

Genetics

Gpr15^tm1.1Litt

Allele Symbol: Gpr15^tm1.1Litt

Allele Name	targeted mutation 1.1, Dan R Littman
Allele Type	Targeted (Reporter, Null/Knockout)
Allele Synonym(s)	Gpr15^gfp
Gene Symbol and Name	Gpr15, G protein-coupled receptor 15
Gene Synonym(s)
Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	EGFP is expressed in lymphocytes of the large intestine lamina propria.
Strain of Origin	129P2/OlaHsd
Chromosome	16
Molecular Note	Part of exon 1 was replaced by GFP and SV40polyA followed by a loxP-flanked neomycin resistance cassette. The neomycin cassette was later removed by Cre treatment. RT-PCR confirmed the absence of transcript expression in the large intestine lamina propria and spleen.
Mutations Made By	Dr. Dan Littman, New York University Medical Center

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Fluorescent Proteins
Neurobiology Research
- Fluorescent protein expression in neural tissue
- Receptor Defects
  - G protein-coupled receptor

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Gpr15^tm1.1Litt/Gpr15^tm1.1Litt
B6.129P2-Gpr15

digestive/alimentary phenotype

increased susceptibility to induced colitis
- mice
- mice infected with Citrobacter rodentium exhibit severe weight loss, increased inflammation, increased tissue damage, increased cytokine expression, decreased regulatory T cell numbers in the large intestine lamina propria and death compared with control mice

immune system phenotype

decreased CD4-positive, alpha beta T cell number
- Normal - however, numbers are normal in the small intestine propria and spleen
- small reduction in the large intestine lamina propria

decreased regulatory T cell number
- in mice infected with Citrobacter rodentium
- reduced percentage and cell number in the large intestine lamina propria
- Normal - however, percentages are normal in the small intestine propria and spleen

increased susceptibility to bacterial infection
- Normal - however, pathogen load in the large intestine and spleen is normal
- mice infected with Citrobacter rodentium exhibit severe weight loss, increased inflammation, increased tissue damage, increased cytokine expression, decreased regulatory T cell numbers in the large intestine lamina propria and death compared with control mice
- mice

increased susceptibility to bacterial infection induced morbidity/mortality
- in mice infected with Citrobacter rodentium

abnormal regulatory T cell physiology
- Normal - however, regulatory T cell exhibit normal ability to suppress naive T cell proliferation
- regulatory T cells transferred into Rag2 null mice fail to reduce induced colitis unlike wild-type cells

increased CD8-positive, alpha-beta T cell number
- Normal - however, the numbers are normal in the small intestine
- in the large intestine lamina propria

increased susceptibility to induced colitis
- mice infected with Citrobacter rodentium exhibit severe weight loss, increased inflammation, increased tissue damage, increased cytokine expression, decreased regulatory T cell numbers in the large intestine lamina propria and death compared with control mice
- mice

decreased double-negative T cell number
- Normal - however, the numbers are normal in the small intestine
- in the large intestine lamina propria

growth/size/body region phenotype

increased susceptibility to weight loss
- severe in mice infected with Citrobacter rodentium

mammalian phenotype

reproductive system phenotype
- Normal - mice are viable and fertile

immune system phenotype
- Background Sensitivity - mice on a congenic C57BL/6 background exhibit normal proportions of IFN-gamma and IL17A-producing CD4+ T cells in the large intestine lamina propria unlike mice on a mixed background

mortality/aging
- Normal - mice are viable and fertile

mortality/aging

increased susceptibility to bacterial infection induced morbidity/mortality
- in mice infected with Citrobacter rodentium

hematopoietic system phenotype

abnormal regulatory T cell physiology
- Normal - however, regulatory T cell exhibit normal ability to suppress naive T cell proliferation
- regulatory T cells transferred into Rag2 null mice fail to reduce induced colitis unlike wild-type cells

decreased double-negative T cell number
- in the large intestine lamina propria
- Normal - however, the numbers are normal in the small intestine

abnormal bone marrow cell physiology
- bone marrow transplanted into irradiated Foxp3^sf mice confers increased susceptibility to Citrobacter rodentium-induced colitis

decreased regulatory T cell number
- in mice infected with Citrobacter rodentium
- Normal - however, percentages are normal in the small intestine propria and spleen
- reduced percentage and cell number in the large intestine lamina propria

decreased CD4-positive, alpha beta T cell number
- small reduction in the large intestine lamina propria
- Normal - however, numbers are normal in the small intestine propria and spleen

increased CD8-positive, alpha-beta T cell number
- Normal - however, the numbers are normal in the small intestine
- in the large intestine lamina propria

The following phenotype relates to a compound genotype created using this strain

Genotype: Gpr15^tm1.1Litt/Gpr15^tm1.1Litt
involves: 129P2/OlaHsd * C57BL/6

hematopoietic system phenotype

decreased regulatory T cell number
- in the large intestine lamina propria

immune system phenotype

increased interferon-gamma secretion
- Background Sensitivity - mice on a mixed background exhibit increased proportion of IFN-gamma and IL17A-producing CD4+ T cells in the large intestine lamina propria compared with mice on a congenic C57BL/6 background

decreased regulatory T cell number
- in the large intestine lamina propria

increased interleukin-17 secretion
- Background Sensitivity - mice on a mixed background exhibit increased proportion of IFN-gamma and IL17A-producing CD4+ T cells in the large intestine lamina propria compared with mice on a congenic C57BL/6 background

References

Additional - Gpr15^tm1.1Litt related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Gpr15
- Genotyping resources and troubleshooting
Breeding Considerations

When maintained as a live colony, heterozygotes or homozygotes may be bred.
- Additional Breeding and Husbandry Support
Citation
When using the B6;129P2-Gpr15^tm1.1Litt/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008769 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
> >	Heterozygous for Gpr15<tm1.1(GFP)Litt>

Related Products and Services

Frozen Mouse Embryo	B6;129P2-Gpr15<tm1.1Litt>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6;129P2-Gpr15<tm1.1Litt>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6;129P2-Gpr15<tm1.1Litt>/J Frozen Embryo	$3373.50
Frozen Mouse Embryo	B6;129P2-Gpr15<tm1.1Litt>/J Frozen Embryo	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Gpr15tm1.1Litt

Allele Symbol: Gpr15tm1.1Litt

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Gpr15tm1.1Litt/Gpr15tm1.1LittB6.129P2-Gpr15

digestive/alimentary phenotype

immune system phenotype

growth/size/body region phenotype

mammalian phenotype

mortality/aging

hematopoietic system phenotype

Genotype: Gpr15tm1.1Litt/Gpr15tm1.1Littinvolves: 129P2/OlaHsd * C57BL/6

hematopoietic system phenotype

immune system phenotype

References

Additional - Gpr15tm1.1Litt related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Gpr15^tm1.1Litt

Allele Symbol: Gpr15^tm1.1Litt

Genotype: Gpr15^tm1.1Litt/Gpr15^tm1.1Litt
B6.129P2-Gpr15

Genotype: Gpr15^tm1.1Litt/Gpr15^tm1.1Litt
involves: 129P2/OlaHsd * C57BL/6

Additional - Gpr15^tm1.1Litt related