B6;129P2-Gpr15^tm1.1Litt/J

Stock number: 008769
Research areas: Neurobiology Research, Research Tools

Description

Green fluorescent protein (GFP) substituted for a portion of exon 1 replaces expression of the Gpr15 (G protein-coupled receptor 15) gene in lymphocytes of the large intestine lamina propria.

Detailed description

Green fluorescent protein (GFP) substituted for a portion of exon 1 replaces expression of the gene in lymphocytes of the large intestine lamina propria. Homozygotes are viable and fertile.

Development

Part of exon 1 was replaced by enhanced green fluorescent protein (EGFP) and SV40polyA followed by a loxP-flanked neomycin resistance cassette. This mutation was created using a 129P2/OlaHsd-derived E14 embryonic stem (ES) cell line. The neomycin cassette was removed by crossing mice with CMV-Cre mice on a BALB/c background. This strain was backcrossed to C57BL/6 for 3 generations by the donating laboratory.

Allele

Symbol: Gpr15^tm1.1Litt
Name: targeted mutation 1.1, Dan R Littman
MGI accession ID: MGI:3815547
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Gpr15
Marker name: G protein-coupled receptor 15
Chromosome: 16
Strain of origin: 129P2/OlaHsd
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: EGFP is expressed in lymphocytes of the large intestine lamina propria.
Molecular note: Part of exon 1 was replaced by GFP and SV40polyA followed by a loxP-flanked neomycin resistance cassette. The neomycin cassette was later removed by Cre treatment. RT-PCR confirmed the absence of transcript expression in the large intestine lamina propria and spleen.