Green fluorescent protein (GFP) substituted for a portion of exon 1 replaces expression of the Gpr15 (G protein-coupled receptor 15) gene in lymphocytes of the large intestine lamina propria.
Dr. Dan R. Littman, New York University Medical Center
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Gpr15 | G protein-coupled receptor 15 |
Green fluorescent protein (GFP) substituted for a portion of exon 1 replaces expression of the gene in lymphocytes of the large intestine lamina propria. Homozygotes are viable and fertile.
Part of exon 1 was replaced by enhanced green fluorescent protein (EGFP) and SV40polyA followed by a loxP-flanked neomycin resistance cassette. This mutation was created using a 129P2/OlaHsd-derived E14 embryonic stem (ES) cell line. The neomycin cassette was removed by crossing mice with CMV-Cre mice on a BALB/c background. This strain was backcrossed to C57BL/6 for 3 generations by the donating laboratory.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | EGFP is expressed in lymphocytes of the large intestine lamina propria. |
Allele Name | targeted mutation 1.1, Dan R Littman |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Gpr15gfp |
Gene Symbol and Name | Gpr15, G protein-coupled receptor 15 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | EGFP is expressed in lymphocytes of the large intestine lamina propria. |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 16 |
Molecular Note | Part of exon 1 was replaced by GFP and SV40polyA followed by a loxP-flanked neomycin resistance cassette. The neomycin cassette was later removed by Cre treatment. RT-PCR confirmed the absence of transcript expression in the large intestine lamina propria and spleen. |
Mutations Made By | Dr. Dan Littman, New York University Medical Center |
When maintained as a live colony, heterozygotes or homozygotes may be bred.
When using the B6;129P2-Gpr15tm1.1Litt/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008769 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Gpr15<tm1.1(GFP)Litt> |
Frozen Mouse Embryo | B6;129P2-Gpr15<tm1.1Litt>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Gpr15<tm1.1Litt>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Gpr15<tm1.1Litt>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129P2-Gpr15<tm1.1Litt>/J Frozen Embryo | $3373.50 |
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