B6.129P2-Cxcr4^tm2Yzo/J

Stock number: 008767
Product name: CXCR4 Flox
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These mice possess loxP sites on either side of exon 2 of the Cxcr4, chemokine (C-X-C motif) receptor 4 gene. When crossed with a cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene.

Detailed description

These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissue(s).

For example, when bred to a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 004126, Stock No. 006785), this mutant mouse strain may be useful in studies of B lymphocyte development.

Development

A loxP-flanked neomycin resistance cassette was inserted into intron 1 and an additional loxP site was placed in intron 2. The targeting vector was introuduced to 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Targeted ES cells were crossed to EIIA-cre transgenic mice to generate animals with a loxP-flanked exon 2. This strain was backcrossed 18 times to C57BL/6 (see SNP note below) by the donating laboratory. Note: The animals sent to The Jackson Laboratory had been additionally crossed to a C57BL/6 background Lck-cre strain (Taconic #4197)and animals retaining the floxed allele (and lacking cre) were imported. Rederivation at JAX involved C57BL/6J mice.

In 2020, a 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. One of the 5 markers that determine C57BL/6J from C57BL/6N was found to be segregating, the rd8 recessive mutation associated with retinal degeneration on Chromosome 1 within the Crb1 locus. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6N;C57BL/6J genetic background.

Allele

Symbol: Cxcr4^tm2Yzo
Name: targeted mutation 2, Yong-Rui Zou
MGI accession ID: MGI:3525214
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Cxcr4
Marker name: chemokine (C-X-C motif) receptor 4
Chromosome: 1
Strain of origin: 129P2/OlaHsd
Molecular note: A neomycin resistance gene flanked by loxP sites was inserted into intron 1 and a third loxP site was introduced into exon 2. Crossing with EIIa-cre mice resulted in the excision of the neo, leaving exon 2 flanked with loxP sites.