FVB-Tg(Ckm-Ppargc1b)T37Brsp/J

Stock number: 008737
Research areas: Metabolism Research, Research Tools

Description

These MCK-PGC1beta transgenic mice (founder line T37) express mouse peroxisome proliferative activated receptor, gamma, coactivator 1 beta under the direction of the mouse muscle creatine kinase promoter. Transgenic mice have an increased number of mitochondria in muscle fibers, increased fast-twitch oxidative type IIX fibers and are able to sustain physical activity longer than wildtype controls. This mutant mouse strain may be useful in studies of muscle physiology and disease, exercise and oxidative capacity, and metabolic homeostasis.

Detailed description

These transgenic mice express mouse peroxisome proliferative activated receptor, gamma, coactivator 1 beta under the direction of the mouse muscle creatine kinase promoter. Transgene expression is specific to skeletal muscle, is highest in "white" glycolytic muscle and low in "red" oxidative muscle. Quantitative real-time PCR analysis reveals transgene transcript is increased approximately 10-fold in quadriceps muscle when compared to wild-type controls. Protein levels are approximately 10-fold greater in gastrocnemius and 3-fold greater in tibialis muscles when compare to wild-type by Western blot analysis. Histological examination shows that muscle fibers in transgenic mice are smaller in size. Transgenic mice have an increased number of mitochondria in muscle fibers and increased fast-twitch oxidative type IIX fibers. Transgenic mice are able to sustain physical activity longer than wild-type controls. Mice that are hemizygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of muscle physiology and disease, exercise and oxidative capacity, and metabolic homeostasis.

Development

A transgenic construct containing the mouse peroxisome proliferative activated receptor, gamma, coactivator 1 beta gene, the 4.8kb mouse muscle creatine kinase promoter and the human growth hormone polyadenylation site was injected into fertilized FVB mouse eggs. Founder line T37 was subsequently established.

Allele

Symbol: Tg(Ckm-Ppargc1b)T37Brsp
Name: transgene insertion T37, Bruce M Spiegelman
MGI accession ID: MGI:4354127
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Ckm-Ppargc1b)T37Brsp
Marker name: transgene insertion T37, Bruce M Spiegelman
Chromosome: UN
Strain of origin: FVB
Expressed genes: Ppargc1b,peroxisome proliferative activated receptor, gamma, coactivator 1 beta,mouse, laboratory
Molecular note: A transgenic construct containing the complete mouse peroxisome proliferative activated receptor, gamma, coactivator 1 beta gene cDNA, the 4.8kb mouse muscle creatine kinase promoter and the human growth hormone polyadenylation site was injected into fertilized FVB mouse eggs. Three lines were generated. Founder line T37 was subsequently established. Expression of Ppargc1b mRNA is increased 10-fold in the quadriceps, and transgene expression is higher in 'white', glycolytic muscle (EDL, quadriceps, gastrocnemius) than in 'red', oxidative muscle (soleus, heart).