These MCK-PGC1beta transgenic mice (founder line T37) express mouse peroxisome proliferative activated receptor, gamma, coactivator 1 beta under the direction of the mouse muscle creatine kinase promoter. Transgenic mice have an increased number of mitochondria in muscle fibers, increased fast-twitch oxidative type IIX fibers and are able to sustain physical activity longer than wildtype controls. This mutant mouse strain may be useful in studies of muscle physiology and disease, exercise and oxidative capacity, and metabolic homeostasis.
Bruce M Spiegelman, Dana-Farber Cancer Institute/Harvard Medical School
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
These transgenic mice express mouse peroxisome proliferative activated receptor, gamma, coactivator 1 beta under the direction of the mouse muscle creatine kinase promoter. Transgene expression is specific to skeletal muscle, is highest in "white" glycolytic muscle and low in "red" oxidative muscle. Quantitative real-time PCR analysis reveals transgene transcript is increased approximately 10-fold in quadriceps muscle when compared to wild-type controls. Protein levels are approximately 10-fold greater in gastrocnemius and 3-fold greater in tibialis muscles when compare to wild-type by Western blot analysis. Histological examination shows that muscle fibers in transgenic mice are smaller in size. Transgenic mice have an increased number of mitochondria in muscle fibers and increased fast-twitch oxidative type IIX fibers. Transgenic mice are able to sustain physical activity longer than wild-type controls. Mice that are hemizygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of muscle physiology and disease, exercise and oxidative capacity, and metabolic homeostasis.
A transgenic construct containing the mouse peroxisome proliferative activated receptor, gamma, coactivator 1 beta gene, the 4.8kb mouse muscle creatine kinase promoter and the human growth hormone polyadenylation site was injected into fertilized FVB mouse eggs. Founder line T37 was subsequently established.
Expressed Gene | Ppargc1b, peroxisome proliferative activated receptor, gamma, coactivator 1 beta, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion T37, Bruce M Spiegelman |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | MCK-PGC-1b; PGC-1b |
Gene Symbol and Name | Tg(Ckm-Ppargc1b)T37Brsp, transgene insertion T37, Bruce M Spiegelman |
Gene Synonym(s) | |
Promoter | Ckm, creatine kinase, muscle, mouse, laboratory |
Expressed Gene | Ppargc1b, peroxisome proliferative activated receptor, gamma, coactivator 1 beta, mouse, laboratory |
Strain of Origin | FVB |
Chromosome | UN |
Molecular Note | A transgenic construct containing the complete mouse peroxisome proliferative activated receptor, gamma, coactivator 1 beta gene cDNA, the 4.8kb mouse muscle creatine kinase promoter and the human growth hormone polyadenylation site was injected into fertilized FVB mouse eggs. Three lines were generated. Founder line T37 was subsequently established. Expression of Ppargc1b mRNA is increased 10-fold in the quadriceps, and transgene expression is higher in 'white', glycolytic muscle (EDL, quadriceps, gastrocnemius) than in 'red', oxidative muscle (soleus, heart). |
Mutations Made By | Bruce Spiegelman, Dana-Farber Cancer Institute/Harvard Medical School |
When maintaining a live colony, these mice can be bred as hemizygotes. The Donating Investigator has not attempted to make the strain homozygous.
When using the FVB-Tg(Ckm-Ppargc1b)T37Brsp/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008737 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Ckm-Ppargc1b)T37Brsp |
Frozen Mouse Embryo | FVB-Tg(Ckm-Ppargc1b)T37Brsp/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(Ckm-Ppargc1b)T37Brsp/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(Ckm-Ppargc1b)T37Brsp/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | FVB-Tg(Ckm-Ppargc1b)T37Brsp/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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