Overview

Also Known As: Twist2-

These Twist2-cre (or, Dermo1-cre) mutant mice harbor a Cre recombinase knock-in allele that also abolishes endogenous twist homolog 2 (Drosophila) (Twist2) gene function. They may be useful for Cre-lox applications/Twist2-deficiency research in embryogenesis of mesoderm-derived tissues (including chondrocytes and osteoblasts), as well as NF-kappaB transcription factor/cytokine signaling pathways.

Donating Investigator

David Ornitz, Washington University School of Medicine

Genetic overview

Genetic Background	Generation
	N11+N1F13 (2019-05-06 00:00:00)

Twist2^{tm1.1(cre)Dor}

Allele Type	Gene Symbol	Gene Name
Targeted (Recombinase-expressing)	Twist2	twist basic helix-loop-helix transcription factor 2

View Genetics

Research Applications

Developmental Biology Research
Research Tools
Dermatology Research
Neurobiology Research
Cancer Research
Immunology, Inflammation and Autoimmunity Research

View All Research Applications

Base Price

Starting at:

$278.00 Domestic price for female 4-week

356.51 Domestic price for breeder pair

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Details

Detailed Description

Twist2-cre (or, Dermo1-cre) mutant mice harbor a Cre recombinase "knock-in" allele that also abolishes endogenous Twist2 gene function. Heterozygotes are viable and fertile, while homozygotes (twist-2^-/-) die a few days after birth. Under control of the upstream promoter/enhancer elements, cre expression is observed in a pattern consistent with the wildtype gene; Cre recombinase activity is reported in mesoderm as early as embryonic day 9.5, in mesodermal tissues such as branchial arches and somites, and in condensed mesenchyme-derived chondrocytes and osteoblasts. When heterozygotes are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequences in Dermo1-expressing tissues of the offspring. Homozygous mice exhibit elevated expression of pro-inflammatory cytokines resulting in perinatal death from cachexia (wasting), as well as progressive growth retardation, impaired movement, thin skin with sparse hair, and severe subcutaneous and internal fat deficiency.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

A targeting vector was designed to replace the first exon of the targeted gene with a cre cDNA sequence and an frt-flanked PGK-neo cassette. The construct was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Chimeric mice were bred to C57BL/6J inbred mice to establish the mutant colony. Mutant mice were then bred to FLPe mice (C57 genetic background) to remove the selection cassette. Mice harboring the Dermo1-cre mutation were then backcrossed to C57BL/6J for at least 10 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Expression Data

Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombinase activity is observed in mesoderm as early as embryonic day 9.5, in mesodermal tissues such as branchial arches and somites, and in condensed mesenchyme-derived chondrocytes and osteoblasts.

Control Suggestions

Wild-type from the colony
000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Sosic D; Richardson JA; Yu K; Ornitz DM; Olson EN. 2003. Twist regulates cytokine gene expression through a negative feedback loop that represses NF-kappaB activity. Cell 112(2):169-80PubMed: 12553906 MGI: J:81485

View All References

Genetics

Twist2^{tm1.1(cre)Dor}

Allele Symbol: Twist2^{tm1.1(cre)Dor}

Allele Name	targeted mutation 1.1, David M Ornitz
Allele Type	Targeted (Recombinase-expressing)
Allele Synonym(s)	Twist2^{tm1.1(cre)Dor}; targeted mutation 1.1, David M Ornitz
Gene Symbol and Name	Twist2, twist basic helix-loop-helix transcription factor 2
Gene Synonym(s)	dermis expressed 1; bHLHa39; DERMO1; FFDD3; SETLSS; Dermo1; Dermo1; BBRSAY; AMS
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombinase activity is observed in mesoderm as early as embryonic day 9.5, in mesodermal tissues such as branchial arches and somites, and in condensed mesenchyme-derived chondrocytes and osteoblasts.
Strain of Origin	129X1/SvJ
Chromosome	1
Molecular Note	A targeting vector was designed to replace the first exon of the targeted gene with a cre cDNA sequence and an frt-flanked PGK-neo cassette. The construct was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Chimeric mice were bred to generate mutant mice. Mutant mice were then bred to FLPe mice (C57 genetic background) to remove the selection cassette.
Mutations Made By	David Ornitz, Washington University School of Medicine

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Developmental Biology Research
- Skin and Hair Texture Defects
- Perinatal Lethality
  - Homozygous
- Mesodermal Defects
- Postnatal Lethality
  - Homozygous
- Growth Defects
  - Growth Defects (homozygous)
Research Tools
- Developmental Biology Research
  - transplantation marker for embryonic and adult tissue
  - Cre-lox System
- Genetics Research
  - Tissue/Cell Markers: transplantation marker for embryonic and adult tissue
  - Tissue/Cell Markers
  - Mutagenesis and Transgenesis
  - Mutagenesis and Transgenesis: Cre-lox System
  - Tissue/Cell Markers: Cre-lox System
- Dermatology Research
- Cre-lox System
  - Cre Recombinase Expression
Dermatology Research
- Skin and Hair Texture Defects
Neurobiology Research
- Ataxia (Movement) Defects
Cancer Research
- Growth Factors/Receptors/Cytokines
Immunology, Inflammation and Autoimmunity Research
- Growth Factors/Receptors/Cytokines

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Twist2^{tm1.1(cre)Dor}/Twist2⁺
involves: 129X1/SvJ * C57BL/6

normal phenotype

no abnormal phenotype detected
- Normal - mice are viable and fertile and do not display any gross physical or behavioral abnormalities

References

Sosic D; Richardson JA; Yu K; Ornitz DM; Olson EN. 2003. Twist regulates cytokine gene expression through a negative feedback loop that represses NF-kappaB activity. Cell 112(2):169-80PubMed: 12553906 MGI: J:81485

Additional - Twist2^{tm1.1(cre)Dor} related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR:Twist2^{tm1.1(cre)Dor}
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

When maintaining a live colony, heterozygous mice may be bred together or to wildtype siblings. Homozygotes die a few days after birth.
- Additional Breeding and Husbandry Support
Mating System
- +/+ sibling x Heterozygote
Citation
When using the Twist2- mouse strain in a publication, please cite the originating article(s) and include JAX stock #008712 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX10 (Standard)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Breeder Pair

Sex

Genotype

Price

Female
Male

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or wild-type for Twist2<tm1.1(cre)Dor>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Terms of Use

General Terms and Conditions

Questions about Terms of Use

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Licensing Information

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Email: TechTran@jax.org

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The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

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Also Known As: Twist2-

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Twist2tm1.1(cre)Dor

Allele Symbol: Twist2tm1.1(cre)Dor

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Twist2tm1.1(cre)Dor/Twist2+involves: 129X1/SvJ * C57BL/6

normal phenotype

References

Additional - Twist2tm1.1(cre)Dor related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Breeder Pair

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Twist2^{tm1.1(cre)Dor}

Allele Symbol: Twist2^{tm1.1(cre)Dor}

Genotype: Twist2^{tm1.1(cre)Dor}/Twist2⁺
involves: 129X1/SvJ * C57BL/6

Additional - Twist2^{tm1.1(cre)Dor} related