These TL-deficient mice harbor mutations in both alleles encoding the non-classical major histocompatibility complex (MHC) class I thymus leukemia (TL) antigen; a targeted "knockout" mutation of the H2-T3 gene (histocompatibility 2, T region locus 3) and also the nonfunctional allele of the H2-T18 gene (histocompatibility 2, T region locus 18) from the C57BL/6 genetic background. Because expression of TL antigen from either locus is absent from intestinal epithelial cells (IEC) and small/large intestine tissues, these TL-deficient mice may be useful in studying thymus leukemia (TL) antigen-deficiency on CD8αα intraepithelial lymphocytes, and the regulation of intraepithelial lymphocyte effector functions (for example, acceleration of chronic colitis and inflammatory bowel disease).
Dr. Luc Van Kaer, Vanderbilt University School of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | H2-T3 | histocompatibility 2, T region locus 3 |
The mouse thymus leukemia (TL) antigen is a nonclassical MHC class I molecule encoded by a locus within the MHC complex harboring two genes; H2-T3 and H2-T18. TL binds with high affinity to the CD8αα molecule expressed on the vast majority of intraepithelial lymphocytes (IEL), and the interaction of CD8αα with TL is important for lymphocyte regulation in the intestine. The C57BL/6 genetic background normally has the functional H2-T3d allele and a mutant H2-T18b allele known to result in no expression on the surface of intestinal epithelial cells (IEC). Because these mutant mice harbor a targeted mutation of the H2-T3 gene that abolishes gene function, expression of TL protein (antibody staining) and RNA (RT-PCR) from either locus is absent in IEC and small/large intestine, respectively. These TL-deficient mice may be useful in studying thymus leukemia (TL) antigen-deficiency on CD8αα intraepithelial lymphocytes, and the regulation of intraepithelial lymphocyte effector functions (for example, acceleration of chronic colitis and inflammatory bowel disease).
A targeting vector was designed to replace part of exon 3 of the targeted gene with a neomycin resistance cassette. The targeting construct was electroporated into C57BL/6-derived BL/6-III embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric males were bred with C57BL/6J females. Mutant mice were then bred together for many generations prior to sending to The Jackson Laboratory. Upon arrival, mice were bred with C57BL/6J inbred mice (Stock No. 000664) to establish the mutant colony.
Allele Name | targeted mutation 1, Luc Van Kaer |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | TL- |
Gene Symbol and Name | H2-T3, histocompatibility 2, T region locus 3 |
Gene Synonym(s) | |
Strain of Origin | C57BL/6J |
Chromosome | 17 |
Molecular Note | Depending on the background strain, thymic leukemia antigen (TL) can be encoded by one to three genes. This locus contains the sole gene encoding TL antigen in C57BL/6 mice. Exon 3 of the gene was disrupted by the insertion of a neomycin resistance cassette. Gene inactivation was confirmed in homozygote C57BL/6 mice by a lack of TL antigen detected by flow cytometry on intestinal epithelium. |
Mutations Made By | Dr. Luc Van Kaer, Vanderbilt University School of Medicine |
When maintaining a live colony, these mice may be bred as homozygotes.
When using the C57BL/6-H2-T3tm1Luc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008711 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for H2-T3<tm1Luc> |
Frozen Mouse Embryo | C57BL/6-H2-T3<tm1Luc>/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-H2-T3<tm1Luc>/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-H2-T3<tm1Luc>/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6-H2-T3<tm1Luc>/J | $3373.50 |
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