B6.129P2(Cg)-Hprt1^{tm4(Ple88-EGFP)Ems}/Mmjax

Stock number: 008706
Strain synonyms: B6.129P2(Cg)-Hprt^{tm4(Ple88-EGFP)Ems}
Research areas: Neurobiology Research, Research Tools

Description

These Ple88-EGFP;mEMS1671 mice (formerly JAX Stock No. 008706) have the Ple88-EGFP transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase 1 (Hprt1) locus on the X chromosome. As the promoter/regulatory regions of the human glial fibrillary acidic protein (GFAP) gene direct expression of enhanced green fluorescent protein (EGFP), these Ple88-EGFP;mEMS1671 mice may be useful in studying GFAP-expressing cells in the brain and diseases affecting the brain.

Allele

Symbol: Hprt1^{tm4(Ple88-EGFP)Ems}
Name: targeted mutation 4, Elizabeth M Simpson
MGI accession ID: MGI:3830628
Generation method: Targeted
Attributes: Reporter
Marker symbol: Hprt1
Marker name: hypoxanthine phosphoribosyltransferase 1
Chromosome: X
Strain of origin: (B6.129P2-Hprt1^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: neural white matter glia and astrocytes
Molecular note: The Ple88-EGFP transgene (pEMS1375) was designed with a Ple88 minipromoter (GFAP-A; a 2210 bp promoter region of the human glial fibrillary acidic protein (GFAP) gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome.

In most cells, EGFP immunoreactivity fills the cell bodies and processes; revealing their full morphology and indicating the glial nature of the cell types. There is EGFP expression in A): astroglial-like cells in the forebrain and cortex, hippocampus and alvius, the cerebellar white matter, as well as the midbrain, brainstem, and eye, B): nascent neuronal/glial progenitor cells in the developing forebrain (including rostral cortical subventricular zone and the rostral migratory stream, and C): numerous cells in the optic nerve and the proximal segment as the nerve enters the retina (much like typical astrocytic staining). While EGFP and GFAP co-expression is observed, not all EGFP+ cells are GFAP+; reflecting the possibility that the Ple88 minipromoter may express in non-glial cell types.
General note: Germ line transmission of mutant cell line mEMS1671 has been established.