Overview

Also Known As:B6.129P2(Cg)-Hprt^{tm4(Ple88-EGFP)Ems}

These Ple88-EGFP;mEMS1671 mice (formerly JAX Stock No. 008706) have the Ple88-EGFP transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase (Hprt) locus on the X chromosome. As the promoter/regulatory regions of the human glial fibrillary acidic protein (GFAP) gene direct expression of enhanced green fluorescent protein (EGFP), these Ple88-EGFP;mEMS1671 mice may be useful in studying GFAP-expressing cells in the brain and diseases affecting the brain.

Donating Investigator

Elizabeth M Simpson, Centre for Molecular Medicine & Therapeutics, University of British Columbia

Genetic overview

Genetic Background	Generation

Hprt^{tm4(Ple88-EGFP)Ems}

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter)	Hprt	hypoxanthine guanine phosphoribosyl transferase

View Genetics

Research Applications

Research Tools
Neurobiology Research

View All Research Applications

Details

Expression Data

Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	neural white matter glia and astrocytes

Selected References

Yang GS; Banks KG; Bonaguro RJ; Wilson G; Dreolini L; de Leeuw CN; Liu L; Swanson DJ; Goldowitz D; Holt RA; Simpson EM. 2009. Next generation tools for high-throughput promoter and expression analysis employing single-copy knock-ins at the Hprt1 locus. Genomics 93(3):196-204PubMed: 18950699 MGI: J:144244

View All References

Genetics

Hprt^{tm4(Ple88-EGFP)Ems}

Allele Symbol: Hprt^{tm4(Ple88-EGFP)Ems}

Allele Name	targeted mutation 4, Elizabeth M Simpson
Allele Type	Targeted (Reporter)
Allele Synonym(s)	Hprt1^{tm4(GFAP-EGFP)Ems}; Hprt1^{tm4(mEMS1671)Ems}; Hprt1^{tm4(Ple88-EGFP;mEMS1671)Ems}; Ple88;GFAP-A;mEMS1671
Gene Symbol and Name	Hprt, hypoxanthine guanine phosphoribosyl transferase
Gene Synonym(s)
Promoter	GFAP, glial fibrillary acidic protein, human
Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	neural white matter glia and astrocytes
Strain of Origin	(B6.129P2-Hprt^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
Chromosome	X
General Note	Germ line transmission of mutant cell line mEMS1671 has been established.
Molecular Note	The Ple88-EGFP transgene (pEMS1375) was designed with a Ple88 minipromoter (GFAP-A; a 2210 bp promoter region of the human glial fibrillary acidic protein (GFAP) gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome. In most cells, EGFP immunoreactivity fills the cell bodies and processes; revealing their full morphology and indicating the glial nature of the cell types. There is EGFP expression in A): astroglial-like cells in the forebrain and cortex, hippocampus and alvius, the cerebellar white matter, as well as the midbrain, brainstem, and eye, B): nascent neuronal/glial progenitor cells in the developing forebrain (including rostral cortical subventricular zone and the rostral migratory stream, and C): numerous cells in the optic nerve and the proximal segment as the nerve enters the retina (much like typical astrocytic staining). While EGFP and GFAP co-expression is observed, not all EGFP+ cells are GFAP+; reflecting the possibility that the Ple88 minipromoter may express in non-glial cell types.
Mutations Made By	Elizabeth Simpson, Centre for Molecular Medicine & Therapeutics, University of British Columbia

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Genetics Research
  - Tissue/Cell Markers: astrocytes
  - Tissue/Cell Markers: astrocytes, neurons
  - Tissue/Cell Markers
  - Tissue/Cell Markers: glial cells
  - Tissue/Cell Markers: neurons
- Fluorescent Proteins
- Neurobiology Research
  - cell marker
Neurobiology Research
- Fluorescent protein expression in neural tissue

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Hprt^{tm4(Ple88-EGFP)Ems}/Y
B6.129P2(Cg)-Hprt/Mmjax

normal phenotype

no abnormal phenotype detected
- Normal - heterozygous females and hemizygous males are viable and fertile

References

Yang GS; Banks KG; Bonaguro RJ; Wilson G; Dreolini L; de Leeuw CN; Liu L; Swanson DJ; Goldowitz D; Holt RA; Simpson EM. 2009. Next generation tools for high-throughput promoter and expression analysis employing single-copy knock-ins at the Hprt1 locus. Genomics 93(3):196-204PubMed: 18950699 MGI: J:144244

Additional - Hprt^{tm4(Ple88-EGFP)Ems} related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Hprt1 STD PCR
- Genotyping resources and troubleshooting
Breeding Considerations

The mutant allele is located on the X chromosome. When maintaining a live colony, homozygous females may be bred with hemizygous males.
- Additional Breeding and Husbandry Support
Citation
When using the B6.129P2(Cg)-Hprt^{tm4(Ple88-EGFP)Ems}/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32918 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

See MMRRC for Additional Conditions of Distribution

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:B6.129P2(Cg)-Hprttm4(Ple88-EGFP)Ems

Donating Investigator

Genetic overview

Research Applications

Expression Data

Selected References

Hprttm4(Ple88-EGFP)Ems

Allele Symbol: Hprttm4(Ple88-EGFP)Ems

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Hprttm4(Ple88-EGFP)Ems/YB6.129P2(Cg)-Hprt/Mmjax

normal phenotype

References

Additional - Hprttm4(Ple88-EGFP)Ems related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Also Known As:B6.129P2(Cg)-Hprt^{tm4(Ple88-EGFP)Ems}

Hprt^{tm4(Ple88-EGFP)Ems}

Allele Symbol: Hprt^{tm4(Ple88-EGFP)Ems}

Genotype: Hprt^{tm4(Ple88-EGFP)Ems}/Y
B6.129P2(Cg)-Hprt/Mmjax

Additional - Hprt^{tm4(Ple88-EGFP)Ems} related