These Ple88-EGFP;mEMS1671 mice (formerly JAX Stock No. 008706) have the Ple88-EGFP transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase (Hprt) locus on the X chromosome. As the promoter/regulatory regions of the human glial fibrillary acidic protein (GFAP) gene direct expression of enhanced green fluorescent protein (EGFP), these Ple88-EGFP;mEMS1671 mice may be useful in studying GFAP-expressing cells in the brain and diseases affecting the brain.
Elizabeth M Simpson, Centre for Molecular Medicine & Therapeutics, University of British Columbia
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter) | Hprt | hypoxanthine guanine phosphoribosyl transferase |
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | neural white matter glia and astrocytes |
Allele Name | targeted mutation 4, Elizabeth M Simpson |
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Allele Type | Targeted (Reporter) |
Allele Synonym(s) | Hprt1tm4(GFAP-EGFP)Ems; Hprt1tm4(mEMS1671)Ems; Hprt1tm4(Ple88-EGFP;mEMS1671)Ems; Ple88;GFAP-A;mEMS1671 |
Gene Symbol and Name | Hprt, hypoxanthine guanine phosphoribosyl transferase |
Gene Synonym(s) | |
Promoter | GFAP, glial fibrillary acidic protein, human |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | neural white matter glia and astrocytes |
Strain of Origin | (B6.129P2-Hprtb-m3/J x 129S-Gt(ROSA)26Sortm1Sor/J)F1 |
Chromosome | X |
General Note | Germ line transmission of mutant cell line mEMS1671 has been established. |
Molecular Note | The Ple88-EGFP transgene (pEMS1375) was designed with a Ple88 minipromoter (GFAP-A; a 2210 bp promoter region of the human glial fibrillary acidic protein (GFAP) gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. In most cells, EGFP immunoreactivity fills the cell bodies and processes; revealing their full morphology and indicating the glial nature of the cell types. There is EGFP expression in A): astroglial-like cells in the forebrain and cortex, hippocampus and alvius, the cerebellar white matter, as well as the midbrain, brainstem, and eye, B): nascent neuronal/glial progenitor cells in the developing forebrain (including rostral cortical subventricular zone and the rostral migratory stream, and C): numerous cells in the optic nerve and the proximal segment as the nerve enters the retina (much like typical astrocytic staining). While EGFP and GFAP co-expression is observed, not all EGFP+ cells are GFAP+; reflecting the possibility that the Ple88 minipromoter may express in non-glial cell types. |
Mutations Made By | Elizabeth Simpson, Centre for Molecular Medicine & Therapeutics, University of British Columbia |
The mutant allele is located on the X chromosome. When maintaining a live colony, homozygous females may be bred with hemizygous males.
When using the B6.129P2(Cg)-Hprttm4(Ple88-EGFP)Ems/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32918 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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