The S5/S6 transmembrane domains are deleted from the transient receptor potential cation channel, subfamily A, member 1 gene. Homozygous mice show reduced sensitivity to pain. This strain may be useful in functional studies of cation channels and the neurological processes of pain.
David P. Corey, Harvard Medical School/HHMI
The S5/S6 transmembrane domains are deleted from the targeted gene. Homozygous mice show reduced sensitivity to pain. They are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This strain may be useful in functional studies of cation channels and the neurological processes of pain.
LoxP sites were placed on either side of the exons that encode for the S5/S6 transmembrane domains of the gene followed by an FRT-flanked self-excising neomycin resistance cassette placed in the opposite transcriptional orientation. The mutation was created with 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. The neomycin cassette was excised through a cross with a Gt(ROSA)26Sor FLP strain on an unknown genetic background and the floxed exons were deleted through a cross with a Cre strain with a widespread pattern of expression on an unknown genetic background. Cre has been selectively bred away from the line.
|Allele Name||targeted mutation 2.1, Kelvin Y Kwan|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Trpa1, transient receptor potential cation channel, subfamily A, member 1|
|Gene Synonym(s)||ANKTM1; Anktm1; FEPS; FEPS1|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||LoxP sites were placed on either side of the exons that encode for the S5/S6 transmembrane domains of the gene followed by an FRT-flanked self-excising neomycin resistance cassette placed in the opposite transcriptional orientation. The neomycin cassette was excised through a cross with a Gt(ROSA)26Sor FLP strain on an unknown genetic background and the floxed exons were deleted through a cross with a Cre strain with a widespread pattern of expression on an unknown genetic background. Cre has been selectively bred away from the line.|
|Mutations Made By|| |
Kelvin Kwan, Harvard Medical School/HHMI
When maintained as a live colony, homozyogotes may be bred.
When using the STOCK Trpa1tm2.1Kykw/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008654 in your Materials and Methods section.
|Heterozygous for Trpa1<tm2.1Kykw>|
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